Parallel Synthesis of Poly(amino ether)-Templated Plasmonic Nanoparticles for Transgene Delivery

Abstract

Plasmonic nanoparticles have been increasingly investigated for numerous applications in medicine, sensing, and catalysis. In particular, gold nanoparticles have been investigated for separations, sensing, drug/nucleic acid delivery, and bioimaging. In addition, silver nanoparticles demonstrate antibacterial activity, resulting in potential application in treatments against microbial infections, burns, diabetic skin ulcers, and medical devices. Here, we describe the facile, parallel synthesis of both gold and silver nanoparticles using a small set of poly(amino ethers), or PAEs, derived from linear polyamines, under ambient conditions and in absence of additional reagents. The kinetics of nanoparticle formation were dependent on PAE concentration and chemical composition. In addition, yields were significantly greater in case of PAEs when compared to 25 kDa poly(ethylene imine), which was used as a standard catonic polymer. Ultraviolet radiation enhanced the kinetics and the yield of both gold and silver nanoparticles, likely by means of a coreduction effect. PAE-templated gold nanoparticles demonstrated the ability to deliver plasmid DNA, resulting in transgene expression, in 22Rv1 human prostate cancer and MB49 murine bladder cancer cell lines. Taken together, our results indicate that chemically diverse poly(amino ethers) can be employed for rapidly templating the formation of metal nanoparticles under ambient conditions. The simplicity of synthesis and chemical diversity make PAE-templated nanoparticles useful tools for several applications in biotechnology, including nucleic acid delivery.

Preparation of Polymer@AuNPs with Droplets Approach for Sensing Serum Copper Ions

“…As a comparison, the PNIPAm@AuNPs synthesized in traditional flasks have been carried out according to the previous work. 19 The synthesis conditions have been optimized, including the reaction time (Figure S5A), the concentrations of HAuCl 4 solution (Figure S5B), and the concentrations of NaOH solution (Figure S5C). The comparisons of UV−vis absorbance (Figure 2A) and size distribution (Figure 2B) of PNIPAm@AuNPs obtained in a conventional flask (Figure 2A-b and Figure 2B-b) and in a droplet microfludic synthesis system (Figure 2A-a and Figure 2B-a) have been displayed.…”

A combined miR-34a and arsenic trioxide nanodrug delivery system for synergistic inhibition of HCC progression after microwave ablation

“…The tumour site is in a weak acid environment; thus, materials with charge reversal used in a weakly acidic environment could be prone to accumulate in the tumour through EPR and further improve the e cacy. PH-sensitive charge reversal polymers based on PAE poly(-amino ester) provide effective drug delivery and control of drug release depending on the pH gradient [16][17][18][19] . This research intends to construct a nanodelivery system with a synergistic effect from carrying As 2 O 3 and miR-34a to inhibit tumour progression after microwave ablation.…”

A combined miR-34a and arsenic trioxide nanodrug delivery system for synergistic inhibition of HCC progression after microwave ablation

“…The tumour site is a weakly acid environment; thus, materials that display charge reversal in a weakly acidic environment could be prone to accumulate in the tumour through the EPR effect and further improve efficacy. pHsensitive charge reversal polymers based on PAEs (poly(-amino esters)) provide effective drug delivery and also control drug release depending on the pH gradient (Chen et al 2015(Chen et al , 2018Yu et al 2014;Ramos et al 2014). This research aimed to construct a nanodelivery system with synergistic effects caused by carrying both As 2 O 3 and miR-34a to inhibit tumour progression after MWA.…”