Parallel optical monitoring of visual signal propagation from the photoreceptors to the inner retina layers

Li, Yi Chao; Strang, Christianne E.; Amthor, Franklin R.; Liu, Lei; Li, Yang Guo; Zhang, Qiu Xiang; Keyser, Kent T.; Yao, Xin Cheng

doi:10.1364/ol.35.001810

Cited by 31 publications

(25 citation statements)

References 13 publications

(15 reference statements)

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“…Moreover, the stimulus-evoked rod OS changes may contribute to stimulus-evoked IOS signals 45 observed in both isolated retinas, 11,17,[46][47][48] intact animals, [49][50][51] and human subjects. Comparative study of the stimulus-evoked TRP and IOS has been discussed in our previous publication.…”

Section: Discussionmentioning

confidence: 99%

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Stimulus-evoked outer segment changes in rod photoreceptors

et al. 2016

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Abstract. Rod-dominated transient retinal phototropism (TRP) has been recently observed in freshly isolated mouse and frog retinas. Comparative confocal microscopy and optical coherence tomography revealed that the TRP was predominantly elicited from the rod outer segment (OS). However, the biophysical mechanism of rod OS dynamics is still unknown. Mouse and frog retinal slices, which displayed a cross-section of retinal photoreceptors and other functional layers, were used to test the effect of light stimulation on rod OSs. Time-lapse microscopy revealed stimulus-evoked conformational changes of rod OSs. In the center of the stimulated region, the length of the rod OS shrunk, while in the peripheral region, the rod OS swung toward the center region. Our experimental observation and theoretical analysis suggest that the TRP may reflect unbalanced rod disc-shape changes due to localized visible light stimulation.

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Section: Discussionmentioning

confidence: 99%

“…Figure 2 shows a schematic diagram of the experimental setup that has been used for intrinsic optical signal (IOS) 17,18 and TRP 10 studies in freshly isolated retinas. A high-speed camera (Neo 5.5, Andor Technology Ltd., Belfast, Ireland) was used for dynamic NIR microscopy imaging.…”

Section: Methodsmentioning

confidence: 99%

Stimulus-evoked outer segment changes in rod photoreceptors

et al. 2016

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“…11 Micrometer level (<3 μm) resolution has been achieved for in vivo IOS imaging of frog retinas using custom-designed confocal 12 and OCT 13 systems. Stimulus-evoked IOSs have been observed in multiple animal models [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] and human subjects. 29 Recent OCT studies revealed rapid IOS changes at photoreceptor outer segments.…”

Section: Introductionmentioning

confidence: 99%

In vivooptical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

Wang

Yao

2016

J. Biomed. Opt

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Abstract. Intrinsic optical signal (IOS) imaging promises a noninvasive method for advanced study and diagnosis of eye diseases. Before pursuing clinical applications, it is essential to understand anatomic and physiological sources of retinal IOSs and to establish the relationship between IOS distortions and eye diseases. The purpose of this study was designed to demonstrate the feasibility of in vivo IOS imaging of mouse models. A high spatiotemporal resolution spectral domain optical coherence tomography (SD-OCT) was employed for depth-resolved retinal imaging. A custom-designed animal holder equipped with ear bar and bite bar was used to minimize eye movements. Dynamic OCT imaging revealed rapid IOS from the photoreceptor's outer segment immediately after the stimulation delivery, and slow IOS changes were observed from inner retinal layers.Comparative photoreceptor IOS and electroretinography recordings suggested that the fast photoreceptor IOS may be attributed to the early stage of phototransduction before the hyperpolarization of retinal photoreceptor.

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“…11,12 SD-OCT has been proven to allow for depth-resolved recording of IOS and to discriminate between different retinal cell populations in isolated frog retinas. 13 In living human eyes, the recording of IOS by highspeed ultrahigh resolution OCT has been possible, however, the recordings of those weak signals suffered from noise and motion artifacts. 14 In the present paper we present the preliminary results of IOS measurements in a healthy subject and two patients with retinal dystrophies, performed by a commercial SD-OCT device.…”

Section: Introductionmentioning

confidence: 99%

Functional imaging of inherited retinal disease with a commercial optical coherence tomography device

et al. 2011

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Retinal dystrophies (RD) are blinding diseases affecting visual acuity mostly at young age. Intrinsic optical signals (IOS) on optical coherence tomography (OCT) may give topographical information on injure of retinal function in these patients. We demonstrate light response of the healthy and diseased human retina by IOS on a commercially available spectral-domain OCT. Significant IOS could be measured in the healthy retina and in unchanged retinal sectors of the RD patients. Main responses were located in the outer retina (photoreceptors) and the nerve fiber layer. In affected areas of RD eyes IOS were significantly reduced or even absent. Functional OCT imaging was able to give information about retinal function in RD patients on a micrometer scale. These results could be of value for refined disease analysis and control of upcoming gene therapy studies.

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Parallel optical monitoring of visual signal propagation from the photoreceptors to the inner retina layers

Cited by 31 publications

References 13 publications

Stimulus-evoked outer segment changes in rod photoreceptors

Stimulus-evoked outer segment changes in rod photoreceptors

In vivooptical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

Functional imaging of inherited retinal disease with a commercial optical coherence tomography device

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