<i>In vivo</i>optical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

Wang, Benquan; Lu, Yiming; Yao, Xincheng

doi:10.1117/1.jbo.21.9.096010

Cited by 29 publications

(28 citation statements)

References 47 publications

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“…3 and 5A]. These IOSs change exhibited an excellent correlation with our previous results [2, 12]. A custom-designed OCT with a high spatiotemporal resolution revealed robust IOSs as early as 1.1 ms after retinal stimulation in in vivo structural and functional imaging of the mouse retina.…”

Section: Discussionsupporting

confidence: 85%

“…The retina is a complex of layered structures, such as the nerve fiber layer, ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, inner segment, outer segment, retinal pigment epithelium and choroid. The major retinal diseases, or endothelial dysfunctions secondary to systemic diseases, can cause retinal dysfunctions that lead to severe vision loss if appropriate treatment is not promptly provided [2]. It is well known that early stages of eye diseases often affect specific retinal layers.…”

Section: Introductionmentioning

confidence: 99%

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Concurrent OCT imaging of stimulus evoked retinal neural activation and hemodynamic responses

Son

Wang

et al. 2017

SPIE Proceedings

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It is well established that major retinal diseases involve distortions of the retinal neural physiology and blood vascular structures. However, the details of distortions in retinal neurovascular coupling associated with major eye diseases are not well understood. In this study, a multi-modal optical coherence tomography (OCT) imaging system was developed to enable concurrent imaging of retinal neural activity and vascular hemodynamics. Flicker light stimulation was applied to mouse retinas to evoke retinal neural responses and hemodynamic changes. The OCT images were acquired continuously during the pre-stimulation, light-stimulation, and post-stimulation phases. Stimulus-evoked intrinsic optical signals (IOSs) and hemodynamic changes were observed over time in blood-free and blood regions, respectively. Rapid IOSs change occurred almost immediately after stimulation. Both positive and negative signals were observed in adjacent retinal areas. The hemodynamic changes showed time delays after stimulation. The signal magnitudes induced by light stimulation were observed in blood regions and did not show significant changes in blood-free regions. These differences may arise from different mechanisms in blood vessels and neural tissues in response to light stimulation. These characteristics agreed well with our previous observations in mouse retinas. Further development of the multi-modal OCT may provide a new imaging method for studying how retinal structures and metabolic and neural functions are affected by age-related macular degeneration (AMD), glaucoma, diabetic retinopathy (DR), and other diseases, which promises novel noninvasive biomarkers for early disease detection and reliable treatment evaluations of eye diseases.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Concurrent OCT imaging of stimulus evoked retinal neural activation and hemodynamic responses

Son

Wang

et al. 2017

SPIE Proceedings

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“…3a, strong stimulation produced accelerated TRP time course, which might reflect more rhodopsin and additional amounts of cascaded reactions activated [23]. Similar effects were also observed in our previous intrinsic optical signal (IOS) and rod OS shrinkage studies, supporting that photoreceptor movement is a major component of IOS and the unbalanced rod OS shrinkage is the mechanical source of TRP [17,24,25]. It is also interesting to note that increased stimulation intensity corresponds to a shortened saturation stage and an accelerated recovery process in the time-magnitude courses of in vivo TRP.…”

Section: Discussionsupporting

confidence: 57%

In vivo observation of transient photoreceptor movement correlated with oblique light stimulation

Liu

Yao

2018

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI

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Rod-dominated transient retinal phototropism (TRP) has been observed in freshly isolated retinas, promising a noninvasive biomarker for high resolution assessment of retinal physiology. However, in vivo mapping of TRP is challenging due to its fast time course and sub-cellular signal magnitude. By developing a line-scanning and virtually structured detection based super-resolution ophthalmoscope, we report here in vivo observation of TRP in frog retina. In vivo characterization of TRP time course and magnitude were implemented by using variable light stimulus intensities.

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“…Functional OCT has been proven able to image intrinsic optical changes (IOS) in the retina in response to visual stimuli. [28][29][30][31][32] Since the fast IOS changes occur on a millisecond scale, while changes in the RBF occur on the scale of seconds, the DOCT protocol we utilized for our current study was not suitable for simultaneous recording of both IOS and RBF changes with the same OCT system. Future development of the OCT technology and image acquisition protocols could allow for simultaneous recording of stimulusinduced IOS and RBF changes in the living retina.…”

Section: Discussionmentioning

confidence: 90%

Correlation of Visually Evoked Functional and Blood Flow Changes in the Rat Retina Measured With a Combined OCT+ERG System

Tan

Mason

MacLellan

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To correlate visually evoked functional and blood flow changes in the rat retina measured simultaneously with a combined optical coherence tomography and electroretinography system (OCTþERG).METHODS. Male Brown Norway (n ¼ 6) rats were dark adapted and anesthetized with ketamine/xylazine. Visually evoked changes in the retinal blood flow (RBF) and functional response were measured simultaneously with an OCTþERG system with 3-lm axial resolution in retinal tissue and 47-kHz image acquisition rate. Both single flash (10 and 200 ms) and flicker (10 Hz, 20% duty cycle, 1-and 2-second duration) stimuli were projected onto the retina with a custom visual stimulator, integrated into the OCT imaging probe. Total axial RBF was calculated from circular Doppler OCT scans by integrating over the arterial and venal flow.RESULTS. Temporary increase in the RBF was observed with the 10-and 200-ms continuous stimuli (~1% and~4% maximum RBF change, respectively) and the 10-Hz flicker stimuli (~8% for 1-second duration and~10% for 2-second duration). Doubling the flicker stimulus duration resulted in~25% increase in the RBF peak magnitude with no significant change in the peak latency. Single flash (200 ms) and flicker (10 Hz, 1 second) stimuli of the same illumination intensity and photon flux resulted in~23 larger peak RBF magnitude and~25% larger RBF peak latency for the flicker stimulus.CONCLUSIONS. Short, single flash and flicker stimuli evoked measureable RBF changes with larger RBF magnitude and peak latency observed for the flicker stimuli.

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In vivooptical coherence tomography of stimulus-evoked intrinsic optical signals in mouse retinas

Cited by 29 publications

References 47 publications

Concurrent OCT imaging of stimulus evoked retinal neural activation and hemodynamic responses

Concurrent OCT imaging of stimulus evoked retinal neural activation and hemodynamic responses

In vivo observation of transient photoreceptor movement correlated with oblique light stimulation

Correlation of Visually Evoked Functional and Blood Flow Changes in the Rat Retina Measured With a Combined OCT+ERG System

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