Paraeutypella guizhouensis gen. et sp. nov. and Diatrypella longiasca sp. nov. (Diatrypaceae) from China

Abstract

In this study, we introduce a novel genus, Paraeutypella, of the family Diatrypaceae comprising three species viz. Paraeutypella guizhouensis sp. nov. and P. citricola (basionym: Eutypella citricola) and P. vitis (basionym: Sphaeria vitis). Diatrypella longiasca sp. nov. is also introduced, which forms a distinct clade in Diatrypella sensu stricto. The discovery of this new genus will contribute to expanding the knowledge and taxonomic framework of Diatrypaceae (Xylariales). Generic delimitations in Diatrypaceae are unsettled because the phylogeny has yet to be resolved using extensive taxon sampling and sequencing of ex-type cultures. During an investigation of xylarialean fungi, we collected eutypella-like fungi which is distinct from Eutypella sensu stricto in our phylogenetic analyses (ITS and β-tubulin), thus, introduced as Paraeutypella guizhouensis gen. et sp. nov.. Paraeutypella is characterised by having 4–25 perithecia in a stroma each with 3–6 sulcate, long ostiolar necks. Paraeutypella citricola comb. nov. (basionym: Eutypella citricola) is introduced on Acer sp. from China. Diatrypella longiasca sp. nov. is introduced as a new species in Diatrypella sensu stricto. which has 2–5 ascomata per stroma and long ascospores, unusual when compared to other Diatrypella species and distinct phylogenetically.

New contributions to Diatrypaceae from karst areas in China

“…Our phylogenetic analyses, based on ITS and β-tubulin, agree with the previous studies ( Acero et al 2004 ; Trouillas et al 2011 ; Mehrabi et al 2015 , 2016 ; de Almeida et al 2016 ; Shang et al 2017 ; Dissanayake et al 2021 ; Zhu et al 2021 ). However, several genera are not monophyletic;for example, Cryptosphaeria , Diatrype , Diatrypella , and Eutypa .…”

“…The ITS sequence data were compared by using NCBI and the result showed that it is 100% similar to the ex-type strain (HVVIT07) of P. citricola . The morphological features of the new collection are consistent with those described by Dissanayake et al (2021) . This collection is identified as a P. citricolca , based on morphological and molecular data.…”

“… Paraeutypella was introduced by Dissanayake et al (2021) to accommodate Paraeutypella guizhouensis and the genus currently comprises three species. The genus is characterised by poorly developed stromata erumpent through the bark, grouped and irregularly shaped, sometimes confluent, dark brown to black, spindle-shaped, 8-spored asci and allantoid, overlapping, subhyaline ascospores ( Trouillas et al 2011 ; de Almeida et al 2016 ; Dissanayake et al 2021 ). In this study, we illustrate Paraeutypella citricola collected from Guizhou Province in China.…”

“…Phylogenetic analyses were performed by searching homologous sequence data of the family Diatrypaceae in the GenBank database, selected from NCBI and recently published papers ( Mehrabi et al 2019 ; Dayarathne et al 2020 ; Konta et al 2020 ; Dissanayake et al 2021 ; Zhu et al 2021 ). After the preliminary identification results of the sequences, multiple sequence alignments ( ITS and β-tubulin) were aligned using BioEdit v. 7.0 ( Hall 1999 ).…”

Figure 3 from: Long S, Liu L, Pi Y, Wu Y, Lin Y, Zhang X, Long Q, Kang Y, Kang J, Wijayawardene NN, Wang F, Shen X, Li Q (2021) New contributions to Diatrypaceae from karst areas in China. MycoKeys 83: 1-37. https://doi.org/10.3897/mycokeys.83.68926

“…Phylogenetic analyses were performed by searching homologous sequence data of the family Diatrypaceae in the GenBank database, selected from NCBI and recently published papers (Mehrabi et al 2019;Dayarathne et al 2020;Konta et al 2020;Dissanayake et al 2021;Zhu et al 2021). After the preliminary identification results of the sequences, multiple sequence alignments (ITS and β-tubulin) were aligned using Bi-oEdit v. 7.0 (Hall 1999).…”