New contributions to Diatrypaceae from karst areas in China

Abstract

In this study, fungal specimens of the family Diatrypaceae were collected from karst areas in Guizhou, Hainan and Yunnan Provinces, China. Morpho-molecular analyses confirmed that these new collections comprise a new genus Pseudodiatrype, three new species (Diatrype lancangensis, Diatrypella pseudooregonensis and Eutypa cerasi), a new combination (Diatrypella oregonensis), two new records (Allodiatrype thailandica and Diatrypella vulgaris) from China and two other known species (Neoeutypella baoshanensis and Paraeutypella citricola). The new taxa are introduced, based on multi-gene phylogenetic analyses (ITS, β-tubulin), as well as morphological analyses. The new genus Pseudodiatrype is characterised by its wart-like stromata with 5–20 ascomata immersed in one stroma and the endostroma composed of thin black outer and inner layers of large white cells with thin, powdery, yellowish cells. These characteristics separate this genus from two similar genera Allodiatrype and Diatrype. Based on morphological as well as phylogenetic analyses, Diatrype lancangensis is introduced as a new species of Diatrype. The stromata of Diatrype lancangensis are similar to those of D. subundulata and D. undulate, but the ascospores are larger. Based on phylogenetic analyses, Diatrype oregonensis is transferred to the genus Diatrypella as Diatrypella oregonensis while Diatrypella pseudooregonensis is introduced as a new species of Diatrypella with 8 spores in an ascus. In addition, multi-gene phylogenetic analyses show that Eutypa cerasi is closely related to E. lata, but the ascomata and asci of Eutypa cerasi are smaller. The polyphyletic nature of some genera of Diatrypaceae has led to confusion in the classification of the family, thus we discuss whether the number of ascospores per asci can still be used as a basis for classification.

Taxonomic and phylogenetic contributions to Diatrypaceae from southeastern Tibet in China

“…Long & Q.R. Li were introduced by Dissanayake et al (2021) and Long et al (2021) , respectively, based on morphological distinctions and polygenic phylogenetic analyses.…”

“…Diatrype , Eutypa, and Eutypella are all unresolved lineages, and phylogenetic studies indicated that the three genera do not form monophyletic groups, even though they clustered within Diatrypaceae ( Hyde et al, 2020 ; Wijayawardene et al, 2020 ; Long et al, 2021 ; Yang et al, 2022 ). In an investigation of the diversity of wood-decaying fungi in southeastern Tibet of China, three undescribed species of diatrypaceous fungi were collected.…”

Morphology and phylogeny of ascomycetes associated with walnut trees (Juglans regia) in Sichuan province, China

“…The comparisons of ITS and tub 2 sequences in NCBI both showed 100% (ITS = 530/530; 0 gaps; tub 2 = 358/358; 0 gaps) similarity to the strain of D. vulgaris (HVGRF03) from holotype specimens. D. vulgaris has been reported in Australia, Thailand, and China ( Trouillas et al, 2011 ; Hyde et al, 2017 ; Long et al, 2021 ). In this study, we introduce D. vulgaris as a new record from Sichuan province in China, and this is the first report on the genus Juglans .…”

“…In the present study, we described 10 species in Dothideomycetes and Sordariomycetes associated walnut trees. Within the broader region of China, there are reports of partial fungi on other known or undescribed plants ( Zhang, 2003 ; Zhu et al, 2016 ; Jayawardena et al, 2018 ; Long et al, 2021 ; Farr and Rossman, 2022 ; Wanasinghe et al, 2022 ; Xu et al, 2022 ; Yang et al, 2022 ). To our knowledge, these are the first accounts of these species in Sichuan province.…”

“…In this study, we described five fungi, D. vulgaris , R. subrufulum , H. juglandinum , H. velutinum , and L. hongheensis , as saprophytes from J. regia . D. vulgaris has been reported in Austria and Thailand ( Trouillas et al, 2011 ; Hyde et al, 2017 ; Long et al, 2021 ), and saprobic in decaying twigs of Rutaceae, Oleaceae, Anacardiaceae, and Vitaceae. Diverse species in Rhytidhysteron are likely to have jumped hosts from surrounding plants and are unlikely to be a specialist, as R. subrufulum has been reported on plants in Rutaceae, Moraceae, Fabaceae, Juglandaceae, and Calycanthaceae.…”