Paradoxical surrogate markers of dental injury-induced pain in the mouse

Gibbs, Jennifer L.; Urban, Rochelle; Basbaum, Allan I.

doi:10.1016/j.pain.2013.04.018

Cited by 23 publications

(52 citation statements)

References 67 publications

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“…However, mice that did not receive acidic saline injections (and the non-responders) steadily increased their chow intake over the same period of time. This finding is similar to a study by Gibbs et al that evaluated the effect of a dental pulp injury on feeding [22]. Mouse body weight, however, did not increase during the time of study after the dental pulp injury.…”

Section: 0 Discussionsupporting

confidence: 90%

“…However, in a study of male mice, no stress effects could be determined using three different pre-clinical pain models involving the hindlimb [21]. In another study of tooth pain in both male and female mice, no differences were found for feeding behavior between sex or after the introduction of a dental pulp injury [22]. In our study of jaw muscle hyperalgesia, no differences were observed for the total number of incisions or the number of episodes of incising.…”

Section: 0 Discussionmentioning

confidence: 99%

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Assessment of incising ethology in the absence and presence of jaw muscle hyperalgesia in a mouse home cage environment

Widmer

Morris-Wiman

2015

Physiology & Behavior

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Introduction Assessment of oral motor behavior in a mouse is challenging due to the lack of currently available techniques that are non-invasive and allow long-term assessment in a home cage environment. The purpose of this study was to evaluate incising behavior using mouse chow attached to a three-dimensional force transducer that was mounted on the existing home cage. In addition, a persistent hyperalgesia condition was introduced to evaluate the sensitivity of the technique to identify incising behavioral changes. Methods Incising activity of CD-1 male and female mice (n=48) was evaluated over a 24 hour recording session during four baseline and six longitudinal hyperalgesia assessment sessions using custom written software. A pre-clinical persistent pain model was used to induce hyperalgesia in the masseter muscle by repetitive acidic saline injections. Sex and age differences were evaluated for multiple incising variables during both light and dark cycles during baseline and hyperalgesia conditions. Results Significant sex differences were found for multiple incising variables but not for age. Discrete incising frequencies were identified in the range of 4.6-10.4 Hz and were reproducibly found in both female and male mice. A significant shift to lower incising frequencies was observed after repetitive acidic saline injections compared to neutral saline injections. This shift to lower frequencies of incising returned to baseline levels after approximately four weeks but was statistically longer in female compared to male mice. Significant differences were also found for chow intake (reduced) and weight change during the hyperalgesia condition. No significant differences were found for total number of incisions or number of incising episodes per day or incising force. Conclusions The findings from this study support the use of recording three dimensional incising forces as a sensitive measure of incising behavior. This novel technique allowed the identification of specific incising variables that were differentially affected in female and male mice during a persistent hyperalgesia. The data were collected in the home cage environment with minimal bias such as experimenter interaction. Similar to other dental pain studies, mice were able to maintain normal incising activity levels per day (total incisions, total number of incising episodes) even in the presence of hyperalgesia.

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confidence: 90%

Section: 0 Discussionmentioning

confidence: 99%

Assessment of incising ethology in the absence and presence of jaw muscle hyperalgesia in a mouse home cage environment

Widmer

Morris-Wiman

2015

Physiology & Behavior

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“…As a common limitation in both the methods, it is arisen the subjectivity of experimenters in measuring facial grooming and head withdrawal behaviors. Measurements of gnawing time using a "Dolognawmeter" (Dolan et al, 2010), sucrose consumption (Gibbs et al, 2013) and thermal operant behavior based on a reward-conflict paradigm using Orofacial Pain Assessment Device (Anderson et al, 2013;Neubert et al, 2005), which represent three recently developed methods for orofacial pain assessments in conscious rodents, enable the assessment of nociception that does not depend on the subjectivity of the experimenters.…”

Section: Discussionmentioning

confidence: 99%

“…Because general anesthetic agents have been reported to affect peripheral nociception and central pain perception (Cornett et al, 2008;Devor and Zalkind, 2001), the results obtained from anesthetized animals are complicated by the potential effects of anesthesia. Recently, several new pain assays have been developed to evaluate intraoral nociception in conscious animals, including measurements of gnawing time, sucrose intake and grimacing (Dolan et al, 2010;Gibbs et al, 2013;Liao et al, 2014). These novel assays have great potential in the evaluation of oral mucosal pain in conscious animals, but special equipment is required to perform these assays.…”

Section: Introductionmentioning

confidence: 99%

Novel methods of applying direct chemical and mechanical stimulation to the oral mucosa for traditional behavioral pain assays in conscious rats

Hitomi

Ono

Miyano³

et al. 2015

Journal of Neuroscience Methods

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“…22 Briefly, mice (18–25 g) were anesthetized by intraperitoneal (i.p.) injection of ketamine-xylazine anesthesia, 100 mg/kg and 10 mg/kg, respectively.…”

Section: Methodsmentioning

confidence: 99%

Molecular, cellular, and behavioral changes associated with pathological pain signaling occur after dental pulp injury

et al. 2017

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Persistent pain can occur after routine dental treatments in which the dental pulp is injured. To better understand pain chronicity after pulp injury, we assessed whether dental pulp injury in mice causes changes to the sensory nervous system associated with pathological pain. In some experiments, we compared findings after dental pulp injury to a model of orofacial neuropathic pain, in which the mental nerve is injured. After unilateral dental pulp injury, we observed increased expression of activating transcription factor 3 (ATF3) and neuropeptide Y (NPY) mRNA and decreased tachykinin precursor 1 gene expression, in the ipsilateral trigeminal ganglion. We also observed an ipsilateral increase in the number of trigeminal neurons expressing immunoreactivity for ATF3, a decrease in substance P (SP) immunoreactive cells, and no change in the number of cells labeled with IB4. Mice with dental pulp injury transiently exhibit hindpaw mechanical allodynia, out to 12 days, while mice with mental nerve injury have persistent hindpaw allodynia. Mice with dental pulp injury increased spontaneous consumption of a sucrose solution for 17 days while mental nerve injury mice did not. Finally, after dental pulp injury, an increase in expression of the glial markers Iba1 and glial fibrillary acidic protein occurs in the transition zone between nucleus caudalis and interpolaris, ipsilateral to the injury. Collectively these studies suggest that dental pulp injury is associated with significant neuroplasticity that could contribute to persistent pain after of dental pulp injury.

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Paradoxical surrogate markers of dental injury-induced pain in the mouse

Cited by 23 publications

References 67 publications

Assessment of incising ethology in the absence and presence of jaw muscle hyperalgesia in a mouse home cage environment

Assessment of incising ethology in the absence and presence of jaw muscle hyperalgesia in a mouse home cage environment

Novel methods of applying direct chemical and mechanical stimulation to the oral mucosa for traditional behavioral pain assays in conscious rats

Molecular, cellular, and behavioral changes associated with pathological pain signaling occur after dental pulp injury

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