Paradoxical effects of the epigenetic modifiers 5-aza-deoxycytidine and suberoylanilide hydroxamic acid on adipogenesis

El‐Serafi, Ahmed T.; Sandeep, Divyasree; Abdallah, Sallam Hasan; Lozansson, Yasmin; Hamad, Moawiah; Khan, Amir Ali

doi:10.1016/j.diff.2019.02.003

Cited by 18 publications

(20 citation statements)

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“…They were then differentiated into adipocytes, chondrocytes, and osteocytes using the StemPro® Adipogenesis differentiation kit (C/N: A1007001, Gibco, Life Technologies, Carlsbad, CA, USA), StemPro® Chondrogenesis differentiation kit (C/N: A1007101, Gibco, Life Technologies, Carlsbad, CA, USA) and the StemPro® Osteogenesis differentiation kit (C/N: A1007201, Gibco, Life Technologies, Carlsbad, CA, USA), respectively, according to manufacturer protocol. Differentiated adipocytes, chondrocytes, and osteocytes were stained with oil red O, Alcian Blue, and Alizarin Red S solution, respectively, as described previously [ 28 ]. Images were visualised under a light microscope and captured using an attached DSLR camera.…”

Section: Methodsmentioning

confidence: 99%

Significant transcriptomic changes are associated with differentiation of bone marrow-derived mesenchymal stem cells into neural progenitor-like cells in the presence of bFGF and EGF

et al. 2020

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Introduction Mesenchymal stem cells (MSCs) isolated from bone marrow have different developmental origins, including neural crest. MSCs can differentiate into neural progenitor-like cells (NPCs) under the influence of bFGF and EGF. NPCs can terminally differentiate into neurons that express beta-III-tubulin and elicit action potential. The main aim of the study was to identify key genetic markers involved in differentiation of MSCs into NPCs through transcriptomic analysis. Method Total RNA was isolated from MSCs and MSCs-derived NPCs followed by cDNA library construction for transcriptomic analysis. Sample libraries that passed the quality and quantity assessments were subjected to high throughput mRNA sequencing using NextSeq®500. Differential gene expression analysis was performed using the DESeq2 R package with MSC samples being a reference group. The expression of eight differentially regulated genes was counter validated using real-time PCR. Results In total, of the 3,252 differentially regulated genes between MSCs and NPCs with two or more folds, 1,771 were upregulated genes, whereas 1,481 were downregulated in NPCs. Amongst these differential genes, 104 transcription factors were upregulated, and 45 were downregulated in NPCs. Neurogenesis related genes were upregulated in NPCs and the main non-redundant gene ontology (GO) terms enriched in NPCs were the autonomic nervous system, cell surface receptor signalling pathways), extracellular structure organisation, and programmed cell death. The main non-redundant GO terms enriched in MSCs included cytoskeleton organisation cytoskeleton structural constituent, mitotic cell cycle), and the mitotic cell cycle process Gene set enrichment analysis also confirmed cell cycle regulated pathways as well as Biocarta integrin pathway were upregulated in MSCs. Transcription factors enrichment analysis by ChEA3 revealed Foxs1 and HEYL, amongst the top five transcription factors, inhibits and enhances, respectively, the NPCs differentiation of MSCs. Conclusions The vast differences in the transcriptomic profiles between NPCs and MSCs revealed a set of markers that can identify the differentiation stage of NPCs as well as provide new targets to enhance MSCs differentiation into NPCs.

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Section: Methodsmentioning

confidence: 99%

Significant transcriptomic changes are associated with differentiation of bone marrow-derived mesenchymal stem cells into neural progenitor-like cells in the presence of bFGF and EGF

et al. 2020

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“…(1-18) days following admission, and required 29 (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36) days to obtain enough keratinocytes to cover 5% of the body surface area with about 0.63 x10 6 cells per TBSA % (approximately 4000 cells/ cm 2 ). Eight patients were treated with cells once, two patients twice, one three times, and one four times.…”

Section: Resultsmentioning

confidence: 99%

“…The media was supplemented with either 10 -6 M retinoic acid and 100 ng/ml epidermal growth factor (EGF). After one week, the media was removed and cells were prepared as described before [18]. The cells were stained with anti-cytokeratin 14 antibody (Abcam, United Kingdom), according to the manufacturer protocol.…”

Section: Page 4/15mentioning

confidence: 99%

Sprayed cultured autologous keratinocytes in the treatment of severe burns: a retrospective matched cohort study

Karlsson

Steinvall

Olofsson

et al. 2019

Preprint

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Objective: Cultured autologous keratinocytes were used to treat burns since 1981 with many challenges and variable clinical outcome. Our aim was to compare the duration of hospital stay between burned patients received skin grafts with and without cultured autologous keratinocytes, in a retrospective cohort study. Multivariable regression was used to analyse associations between duration of hospital stay and treatment adjusted for age, mortality, size, and depth of the burn. Then, we investigated the differentiation of human stem cell line to keratinocyte-like cells as a future perspective. Results : The median hospital stay in the group given cultured autologous keratinocytes (n=12) was 70 days (10 th – 90 th centile 50-141) and in the matched group 62 days (2 -196) (n=43), p=0.25. The multivariable regression analysis showed a coefficient of 17.36 (95 % CI -17.69 to 52.40), p= 0.32, for hospital stay in the treatment group, compared with the matched group. Our results showed no difference in the duration of hospital stay between the two groups. Therefore, further evaluation for this treatment is needed. Stem cells showed enhanced proliferation and differentiation when cultured on glass surface rather than the classical tissue culture plastic and should be considered for burn management research.

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“…49 The effect of the shapes also influences the gold nanoparticles uptake. 29 Other studies revealed that net positive charge enhances the internalization of the nanoparticles by this route. 50 The differences were also found in the internalization of nanoparticles with different coats.…”

Section: Cell Studiesmentioning

confidence: 98%

Protein-Coated Aryl Modified Gold Nanoparticles for Cellular Uptake Study by Osteosarcoma Cancer Cells

et al. 2020

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Gold nanoparticles coated with proteins have shown extraordinary biocompatibility which advanced to several nanomedicine engineering applications. We synthesized protein-coated gold nanoparticles using green and chemical reduction routes for cellular uptake study. In the current work, we coated gold-aryl nanoparticles of the type AuNPs-C 6 H 4-4-COOH with BSA, collagen, zein and lysozyme proteins. Both routes were carried out without phase-transfer catalysts or extraneous stabilizing agents. High crystallinity of the AuNPs synthesized by the green route can be seen in the transmission electron microscopy images. Osteosarcoma cancer cells are malignant bone tumors with abnormal cellular functions. Studies using MG-63 cells will provide mechanistic suggestions on the details of the amplification in tumors. We studied the cellular uptake of the bioconjugates by MG-63 osteosarcoma cells using laser confocal fluorescence microscopy (LCFM) and flow cytometry. In the LCFM study, BSA-AuNPs was uptaken most efficiently of all protein-coated gold nanoparticles synthesized by the green route. Zein and lysozyme coated nanoparticles, though small sizes, prepared by the green method were not efficiently uptaken by MG-63. The two nanoparticles are negatively charged and zein is also a hydrophobic coat. The difference in hydrophobicity and charge might have affected the internalization. All of those coated nanoparticles that were efficiently uptaken can potentially be used as diagnostic and therapeutic agents for osteosarcoma.

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Paradoxical effects of the epigenetic modifiers 5-aza-deoxycytidine and suberoylanilide hydroxamic acid on adipogenesis

Cited by 18 publications

References 50 publications

Significant transcriptomic changes are associated with differentiation of bone marrow-derived mesenchymal stem cells into neural progenitor-like cells in the presence of bFGF and EGF

Significant transcriptomic changes are associated with differentiation of bone marrow-derived mesenchymal stem cells into neural progenitor-like cells in the presence of bFGF and EGF

Sprayed cultured autologous keratinocytes in the treatment of severe burns: a retrospective matched cohort study

Protein-Coated Aryl Modified Gold Nanoparticles for Cellular Uptake Study by Osteosarcoma Cancer Cells

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