Paracrine signaling of human mesenchymal stem cell modulates retinal microglia population number and phenotype in vitro

Teixeira-Pinheiro, Leandro Coelho; Toledo, María Fernanda; Nascimento-dos-Santos, Gabriel; Mendez-Otero, Rosália; Mesentier-Louro, Louise A.; Santiago, Marcelo F.

doi:10.1016/j.exer.2020.108212

Cited by 8 publications

(4 citation statements)

References 43 publications

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“…After co-culturing with MSC-derived microvesicles, increased levels of M2 markers and fewer LPS-induced pro-inflammatory cytokines were detected in BV-2 cells, indicating their role in the phenotypic transition from M1 to M2 ( 163 ). To observe the role of MSCs in the retina, MSCs obtained from human umbilical cords were co-cultured with complete adult rat retinal explants and kept apart using a transwell membrane ( 164 ). Due to a decline in M1 markers and a raise in M2 markers, paracrine signaling of hMSC altered the microglial phenotype and the production of anti-inflammatory proteins in the retina ( 164 ).…”

Section: New Strategies For Dr Treatment Targeting Polarizationmentioning

confidence: 99%

“…To observe the role of MSCs in the retina, MSCs obtained from human umbilical cords were co-cultured with complete adult rat retinal explants and kept apart using a transwell membrane ( 164 ). Due to a decline in M1 markers and a raise in M2 markers, paracrine signaling of hMSC altered the microglial phenotype and the production of anti-inflammatory proteins in the retina ( 164 ). In neurological studies, MSCs have been found to induce M2 polarization in macrophages and facilitate their migration into MSCs in vitro ( 165 ).…”

Section: New Strategies For Dr Treatment Targeting Polarizationmentioning

confidence: 99%

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Macrophage/microglia polarization for the treatment of diabetic retinopathy

Yao,

Li,

Zhou

et al. 2023

Front. Endocrinol.

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Macrophages/microglia are immune system defense and homeostatic cells that develop from bone marrow progenitor cells. According to the different phenotypes and immune responses of macrophages (Th1 and Th2), the two primary categories of polarized macrophages/microglia are those conventionally activated (M1) and alternatively activated (M2). Macrophage/microglial polarization is a key regulating factor in the development of inflammatory disorders, cancers, metabolic disturbances, and neural degeneration. Macrophage/microglial polarization is involved in inflammation, oxidative stress, pathological angiogenesis, and tissue healing processes in ocular diseases, particularly in diabetic retinopathy (DR). The functional phenotypes of macrophages/microglia affect disease progression and prognosis, and thus regulate the polarization or functional phenotype of microglia at different DR stages, which may offer new concepts for individualized therapy of DR. This review summarizes the involvement of macrophage/microglia polarization in physiological situations and in the pathological process of DR, and discusses the promising role of polarization in personalized treatment of DR.

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Section: New Strategies For Dr Treatment Targeting Polarizationmentioning

confidence: 99%

Section: New Strategies For Dr Treatment Targeting Polarizationmentioning

confidence: 99%

Macrophage/microglia polarization for the treatment of diabetic retinopathy

Yao,

Li,

Zhou

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

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“…With regard to retinal disease, a paracrine effect has been shown to promote survival of injured RGCs and to reduce nerve gliosis, whereas a paracrine factor could provide neuroprotection and neurotrophy. More fascinating features are discussed below [ 98 , 99 , 100 ].…”

Section: Mesenchymal Stem Cell (Msc)mentioning

confidence: 99%

Current Development, Obstacle and Futural Direction of Induced Pluripotent Stem Cell and Mesenchymal Stem Cell Treatment in Degenerative Retinal Disease

Chiang

Chern

2022

IJMS

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Degenerative retinal disease is one of the major causes of vision loss around the world. The past several decades have witnessed emerging development of stem cell treatment for retinal disease. Nevertheless, sourcing stem cells remains controversial due to ethical concerns and their rarity. Furthermore, induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs) are both isolated from patients’ mature tissues; thus, issues such as avoiding moral controversy and adverse events related to immunosuppression and obtaining a large number of cells have opened a new era in regenerative medicine. This review focuses on the current application and development, clinical trials, and latest research of stem cell therapy, as well as its limitations and future directions.

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“…To date, many stem cell treatments have been shown to exert regulatory effects on M1 microglia both in vitro and in vivo . By coculturing the retinas of adult rats with human BMSCs (hBMSCs) in vitro , researchers found that the number of CD68 + ameboid microglia decreased and the loss of RGCs was prevented ( Teixeira-Pinheiro et al, 2020 ). Consistently, the activation of CD11b + M1 microglia was inhibited by coculturing with MSC-derived microvesicles in an in-vitro model.…”

Section: Immunomodulatory Effects Of Stem Cell Therapy Targeting Microgliamentioning

confidence: 99%

Shaping the Microglia in Retinal Degenerative Diseases Using Stem Cell Therapy: Practice and Prospects

Jin

Sha

Gao

2021

Front. Cell Dev. Biol.

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Retinal degenerative disease (RDD) refers to a group of diseases with retinal degeneration that cause vision loss and affect people’s daily lives. Various therapies have been proposed, among which stem cell therapy (SCT) holds great promise for the treatment of RDDs. Microglia are immune cells in the retina that have two activation phenotypes, namely, pro-inflammatory M1 and anti-inflammatory M2 phenotypes. These cells play an important role in the pathological progression of RDDs, especially in terms of retinal inflammation. Recent studies have extensively investigated the therapeutic potential of stem cell therapy in treating RDDs, including the immunomodulatory effects targeting microglia. In this review, we substantially summarized the characteristics of RDDs and microglia, discussed the microglial changes and phenotypic transformation of M1 microglia to M2 microglia after SCT, and proposed future directions for SCT in treating RDDs.

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Paracrine signaling of human mesenchymal stem cell modulates retinal microglia population number and phenotype in vitro

Cited by 8 publications

References 43 publications

Macrophage/microglia polarization for the treatment of diabetic retinopathy

Macrophage/microglia polarization for the treatment of diabetic retinopathy

Current Development, Obstacle and Futural Direction of Induced Pluripotent Stem Cell and Mesenchymal Stem Cell Treatment in Degenerative Retinal Disease

Shaping the Microglia in Retinal Degenerative Diseases Using Stem Cell Therapy: Practice and Prospects

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