Paracrine effect of inflammatory cytokine-activated bone marrow mesenchymal stem cells and its role in osteoblast function

Li, Cheng; Li, Guoqiang; Li, Meng; Zhou, Tiantian; Zhou, Haibin

doi:10.1016/j.jbiosc.2015.05.017

Cited by 84 publications

(61 citation statements)

References 43 publications

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“…IL-6 is a cytokine that is typically considered as pro-inflammatory but in certain conditions it could act as an anti-inflammatory cytokine and can promote angiogenesis as well, and IL-10 is an anti-inflammatory cytokine that contributes to the downregulation of T cell proliferation [37,60,61]. The increase in IL-6 and the lack of increase in IL-10 secretion by MSCs due to inflammation has been observed previously, and the antimicrobials had no effect on MCS IL-6 and IL-10 secretion [62]. ICAM-1 is a critical MSC immunosuppressive molecule that is produced by MSCs in the obligatory presence of IFN-γ, and produced in larger amounts from a synergistic effect with TNF-α [63].…”

Section: Discussionmentioning

confidence: 84%

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels

et al. 2017

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Mesenchymal stromal/stem cells (MSCs) have demonstrated pro-healing properties including an anti-inflammatory cytokine profile and the promotion of angiogenesis via expression of growth factors in pre-clinical models. MSCs encapsulated in poly(ethylene glycol) diacrylate (PEGdA) and thiolated gelatin poly(ethylene glycol) (Gel-PEG-Cys) crosslinked hydrogels have led to controlled cellular presentation at wound sites with favorable wound healing outcomes. However, the therapeutic potential of MSC-loaded hydrogels may be limited by non-specific protein adsorption on the delivery matrix that could facilitate the initial adhesion of microorganisms and subsequent virulent biofilm formation. Antimicrobials loaded concurrently in the hydrogels with MSCs could reduce microbial bioburden and promote healing, but the antimicrobial effect on the MSC wound healing capacity and the antibacterial efficacy of the hydrogels is unknown. We demonstrate that minocycline specifically induces a favorable change in MSC migration capacity, proliferation, gene expression, extracellular matrix (ECM) attachment, and adhesion molecule and growth factor release with subsequent increased angiogenesis. We then demonstrate that hydrogels loaded with MSCs, minocycline, vancomycin, and linezolid can significantly decrease bacterial bioburden. Our study suggests that minocycline can serve as a dual mechanism for the regenerative capacity of MSCs and the reduction of bioburden in triple antimicrobial-loaded hydrogels.

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Section: Discussionmentioning

confidence: 84%

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels

et al. 2017

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“…We hypothesize that this beneficial effect was based on an autocrine regulation by MSCs cells themselves. Indeed, several studies have shown that MSCs can regulate their differentiation, towards the osteoblastic lineage, via an autocrine/paracrine process by the secretion of interferon gamma and tumor necrosis factor alpha 27, 28 . We postulate that the disk cellular arrangement could sustain, due to the proximity and higher homogeneity of cell distribution, a paracrine regulation of MSCs differentiation towards the osteoblastic lineage.…”

Section: Discussionmentioning

confidence: 99%

In situ printing of mesenchymal stromal cells, by laser-assisted bioprinting, for in vivo bone regeneration applications

Kériquel

Oliveira

Rémy

et al. 2017

Sci Rep

323

242

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Bioprinting has emerged as a novel technological approach with the potential to address unsolved questions in the field of tissue engineering. We have recently shown that Laser Assisted Bioprinting (LAB), due to its unprecedented cell printing resolution and precision, is an attractive tool for the in situ printing of a bone substitute. Here, we show that LAB can be used for the in situ printing of mesenchymal stromal cells, associated with collagen and nano-hydroxyapatite, in order to favor bone regeneration, in a calvaria defect model in mice. Also, by testing different cell printing geometries, we show that different cellular arrangements impact on bone tissue regeneration. This work opens new avenues on the development of novel strategies, using in situ bioprinting, for the building of tissues, from the ground up.

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Section: Pro-inflammatory Statementioning

confidence: 99%

“…Interestingly, pro-inflammatory conditions may increase expression of osteogenic genes such as ALP and result in increased mineralization (139, 140). Li et al further demonstrated that conditioned medium from TNF-α-activated MSCs can enhance osteogenesis through paracrine mechanisms (139). However, Sidney and colleagues demonstrated decreases in cell viability and reduction in formation of bone nodules by primary osteoblasts in response to cytokine stimulation (141).…”

Section: Pro-inflammatory Statementioning

confidence: 99%

The Effects of Endocrine Disruptors on Adipogenesis and Osteogenesis in Mesenchymal Stem Cells: A Review

et al. 2017

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Endocrine-disrupting chemicals (EDCs) are prevalent in the environment, and epidemiologic studies have suggested that human exposure is linked to chronic diseases, such as obesity and diabetes. In vitro experiments have further demonstrated that EDCs promote changes in mesenchymal stem cells (MSCs), leading to increases in adipogenic differentiation, decreases in osteogenic differentiation, activation of pro-inflammatory cytokines, increases in oxidative stress, and epigenetic changes. Studies have also shown alteration in trophic factor production, differentiation ability, and immunomodulatory capacity of MSCs, which have significant implications to the current studies exploring MSCs for tissue engineering and regenerative medicine applications and the treatment of inflammatory conditions. Thus, the consideration of the effects of EDCs on MSCs is vital when determining potential therapeutic uses of MSCs, as increased exposure to EDCs may cause MSCs to be less effective therapeutically. This review focuses on the adipogenic and osteogenic differentiation effects of EDCs as these are most relevant to the therapeutic uses of MSCs in tissue engineering, regenerative medicine, and inflammatory conditions. This review will highlight the effects of EDCs, including organophosphates, plasticizers, industrial surfactants, coolants, and lubricants, on MSC biology.

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Paracrine effect of inflammatory cytokine-activated bone marrow mesenchymal stem cells and its role in osteoblast function

Cited by 84 publications

References 43 publications

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels

In situ printing of mesenchymal stromal cells, by laser-assisted bioprinting, for in vivo bone regeneration applications

The Effects of Endocrine Disruptors on Adipogenesis and Osteogenesis in Mesenchymal Stem Cells: A Review

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