Paracoccus alkanivorans sp. nov., isolated from a deep well with oil reservoir water

A novel facultatively anaerobic, non-motile, Gram-stain-negative, non-endospore-forming alphaproteobacterium, strain 1011MAR3C25T, was isolated from a white biofilm colonizing the walls of the Andalusian show cave Gruta de las Maravillas (Huelva, Spain). Strain 1011MAR3C25T grew at 8–42 °C (optimum, 20–30 °C), at pH 5.0–9.0 (optimum, pH 5.0–6.0) and in the presence of 0–12 % (w/v) NaCl (optimum 3–5 %). Cells were catalase- and oxidase-positive. The strain grew heterotrophically with various carbon sources and chemoautotrophically with thiosulfate under aerobic conditions. Results of phylogenetic analysis showed that strain 1011MAR3C25T was related to Paracoccus saliphilus DSM 18447T and Paracoccus alkanivorans LMG 30882T (97.90 % and 97.32 % 16S rRNA sequence identity values, respectively). The major respiratory quinone was ubiquinone Q-10 and the predominant fatty acid was C18 : 1 ω7c. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid and an unidentified polar lipid. The DNA G+C content was 60.3 mol%. Based on a polyphasic taxonomic study it is proposed that strain 1011MAR3C25T (=CECT 9092T=LMG 29414T) represents a novel species of the genus Paracoccus , for which the name Paracoccus onubensis sp. nov. is proposed.

show abstract

“…The midi system groups these fatty acids together as one feature with a single percentage of the total. †Data from Zhang et al . [41]. …”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…*a, Wang et al[6]; b, Liu et al[39]; c, Kim et al[40]; d, Roh et al[3]; e, Zhang et al[41].DESCRIPTION OF PARACOCCUS ONUBENSIS SP. NOV.Paracoccus onubensis ( o. nu′ ben.…”

mentioning

confidence: 99%

Paracoccus onubensis sp. nov., a novel alphaproteobacterium isolated from the wall of a show cave

Gutierrez-Patricio

Gonzalez-Pimentel

Miller

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…In addition, bacteria of the genus Paracoccus were isolated from other various habitats, such as air (Xue et al 2017), soil (Sun et al 2015), sediment (Zhang et al 2019), seawater (Lyu et al 2021) and sediment of freshwater (Wang et al 2009). And they also exist in some plants (Rai et al 2020), animal species (Kim et al 2018; Lee et al 2019) and some extreme environments, such as hot spring (Ye et al 2020), plateau permafrost (Zhu et al 2013) and even oilcontaminated water (Zhang et al 2020b). Many Paracoccus spp.…”

Section: Introductionmentioning

confidence: 99%

Paracoccus marinaquae sp. nov., isolated from coastal water of the Yellow Sea

Xue

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

A Gram-stain-negative, non-motile and coccoid bacterial strain, designated XHP0099T, was isolated from coastal water of the Yellow Sea, China. Growth occurred at 20–37°C (optimum, 28°C), pH 5.0–9.0 (optimum, pH 7.0–8.0), and with 0–7.0% NaCl (optimum, 2.0–3.0%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XHP0099T was related to members of the genus Paracoccus and shared highest sequence similarity with P. siganidrum M26T (98.2%), followed by P. alkanivorans 4-2T (97.6%) and P. alkenifer DSM 11593T (97.4%). The major cellular fatty acids (＞10%) were summed feature 8 (C18:1 ω7c/C18:1 ω6c), and C18:0. The major isoprenoid quinone was Q-10 and the polar lipids contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), aminolipid (AL) and unidentified polar lipid (L). The average nucleotide identity values between strain XHP0099T and P. siganidrum M26T, P. alkanivorans 4-2T, P. alkenifer DSM 11593T and P. saliphilus DSM 18447T were 81.41%, 80.13%, 75.77% and 78.27%, respectively. The digital DNA–DNA hybridization values between strain XHP0099T with P. siganidrum M26T, P. alkanivorans 4-2T, P. alkenifer DSM 11593T and P. saliphilus DSM 18447T were 24.10%, 23.00%, 20.80 % and 21.20%, respectively. The G+C content of the genomic DNA of strain XHP0099T was 66.0%. Based on the phenotypic, phylogenetic, biochemical and chemotaxonomic analysis, strain XHP0099T represents a novel species of the genus Paracoccus, for which the name Paracoccus marinaquae sp. nov. is proposed. The type strain is XHP0099T (=JCM 34661T=GDMCC 1.2414T=MCCC 1K05846T).

show abstract

“…To date, the genus Paracoccus has 77 validly published names (https://lpsn.dsmz.de/genus/paracoccus) and Paracoccus denitrificans is the type species [1]. Previous studies have shown that the members of the genus Paracoccus are isolated from various environments like the wall of a show cave [5], a deep well with oil reservoir water [6], the rhizosphere of an orchid [7], horse blood [8], sea sand [9], marine sediment [2], forest soil [10], root nodules [11], sludge [12], tidal flat [13], mangrove [14] and a pig farm dust collector [15]. Members are Gram-negative, oxidase-positive and catalase-positive [16].…”

Section: Introductionmentioning

confidence: 99%

Paracoccus everestensis sp. nov., a novel bacterium with great antioxidant capacity isolated from the north slope of Mount Everest

Cui

Liu

Xu³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A bacterial strain, designated S8-55T, was isolated from moraine samples collected from the north slope of Mount Everest at an altitude of 5 500 m above sea level. The purpose of this study was to describe a novel species and its characteristics, through genome sequencing and analysis of the relationship between the members of the genus Paracoccus , and explore the antioxidant capacity of strain S8-55T. The polyphasic study confirmed the affiliation of strain S8-55T with the genus Paracoccus . Strain S8-55T was aerobic, Gram-negative and oxidase- and catalase positive. Cells were orange-pigmented, ellipsoid and had no spore formation, no flagella and no motility. Strain S8-55T grow at 10–37 °C, pH 7–11 and without NaCl. Ubiquinone 10 was its predominant respiratory menaquinone. The polar lipids of strain S8-55T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipid, an unidentified aminolipid and three unidentified lipids. Its major fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The G+C content was 64.3 mol%. The phylogenetic analysis based on the 16S rRNA sequence showed that strain S8-55T was closely related to Paracoccus angustae E6T (97.9 %), Paracoccus aerius 011410T (97.9 %) and Paracoccus hibisci THG-T2.8T (97.8 %). The average nucleotide identity values among strain S8-55T and P. angustae CCTCC AB 2015056T, P. aerius KCTC 42845T and P. hibisci CCTCC AB 2016181T were 84.1, 84.5 and 76.3 %, respectively. The genome of strain S8-55T contained antioxidant genes such as oxyR, recD, katE, recD and rpoH. Based on its morphological, physiological and chemical taxonomic characteristics, strain S8-55T (=JCM 35 227T=GDMCC 1.3026T) should be classified as a novel species of the genus Paracoccus with the proposed name Paracoccus everestensis sp. nov.

show abstract

Paracoccus alkanivorans sp. nov., isolated from a deep well with oil reservoir water

Cited by 14 publications

References 31 publications

Paracoccus onubensis sp. nov., a novel alphaproteobacterium isolated from the wall of a show cave

Paracoccus onubensis sp. nov., a novel alphaproteobacterium isolated from the wall of a show cave

Paracoccus marinaquae sp. nov., isolated from coastal water of the Yellow Sea

Paracoccus everestensis sp. nov., a novel bacterium with great antioxidant capacity isolated from the north slope of Mount Everest

Contact Info

Product

Resources

About