Paracoccus onubensis sp. nov., a novel alphaproteobacterium isolated from the wall of a show cave

Abstract

A novel facultatively anaerobic, non-motile, Gram-stain-negative, non-endospore-forming alphaproteobacterium, strain 1011MAR3C25T, was isolated from a white biofilm colonizing the walls of the Andalusian show cave Gruta de las Maravillas (Huelva, Spain). Strain 1011MAR3C25T grew at 8–42 °C (optimum, 20–30 °C), at pH 5.0–9.0 (optimum, pH 5.0–6.0) and in the presence of 0–12 % (w/v) NaCl (optimum 3–5 %). Cells were catalase- and oxidase-positive. The strain grew heterotrophically with various carbon sources and chemoautotrophically with thiosulfate under aerobic conditions. Results of phylogenetic analysis showed that strain 1011MAR3C25T was related to Paracoccus saliphilus DSM 18447T and Paracoccus alkanivorans LMG 30882T (97.90 % and 97.32 % 16S rRNA sequence identity values, respectively). The major respiratory quinone was ubiquinone Q-10 and the predominant fatty acid was C18 : 1 ω7c. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid and an unidentified polar lipid. The DNA G+C content was 60.3 mol%. Based on a polyphasic taxonomic study it is proposed that strain 1011MAR3C25T (=CECT 9092T=LMG 29414T) represents a novel species of the genus Paracoccus , for which the name Paracoccus onubensis sp. nov. is proposed.

Airborne bacteria in show caves from Southern Spain

“…This bacterium was isolated and described from a Chinese cave [55]. The data indicate that caves are reservoirs for novel species of bacteria [34,35,[54][55][56] as shown by the isolation of a novel Paracoccus onubensis from the walls of Gruta de las Maravillas [57].…”

Abyssibius alkaniclasticus gen. nov., sp. nov., a novel member of the family Rhodobacteraceae, isolated from the Mariana Trench

“…Table 1. Differentiating characteristics of strain 960558 T and the most closely related genera of the family Rhodobacteraceae Taxa: 1, strain 960558 T (in this study); 2, Planktotalea [40][41][42]; 3, Nioella [43][44][45][46]; 4, Paracoccus [47][48][49][50][51][52]; 5, Pseudohalocynthiibacter [12]; 6, Pacificibacter [53][54][55][56]. For genera 2-6, all data are obtained from previous studies.…”

Whole-genome sequencing of Paracoccus species isolated from the healthy human eye and description of Paracoccus shanxieyensis sp. nov

“…Transmission electron microscope and scanning electron microscope images are shown in Figs S4 and S5, respectively. Strains DK398 T and DK608 were found to be catalase- and oxidase-positive based on the observation of bubble production on 3 % (v/v) H 2 O 2 and oxidase testing in an identification tube, respectively [3]. API ZYM (BioMérieux, France), API 20E (BioMérieux, France), API 20NE (BioMérieux, France), and API 50CH (BioMérieux, France) strips were used to examine the biochemical features of the two strains.…”

“… Paracoccus forms a group of Gram-negative, coccoid, ovoid or short-rod-shaped, non-spore-forming, and non-motile cells [2]. Currently, the genus Paracoccus comprises 76 recognized species with validly published names (LPSN, https://lpsn.dsmz.de/genus/paracoccus), including two relatively new additions: Paracoccus onubensis 1011MAR3C25 T [3] and Paracoccus salsus EGI L200073 T [4]. Members of Paracoccus are mostly isolated from environmental sources such as sludge [5], soil [6], and sediment [7], and some from animals [8] and plants [9].…”