Paper chromatography of alkaloids and their transformation products in Maryland tobacco

Studies on Tobacco Alkaloids. I. Changes in Nicotine and Nornicotine Content in Nicotiana.

“…Its absorption spectrum showed a minimum at 246 m,u and a maximum at 260 my. A yellowish-brown color was produced with PABA-CNBr (16). On the basis of these results it appeared to be a pyridine derivative.…”

“…Sam-ples were taken at different time intervals until January 18,1954. These plants were also extracted with 50 % acetone, taking into consideration the water content in the plant tissue; and the alkaloids were separated and estimated as before (16 Table I shows the results obtained from seeds of N. glutinosa and Robinson tobacco. No alkaloids or related compounds were detected in the seed of Robinson, but nicotinic acid, an unknown compound, and a very small amount of nicotine were found in the seed of N. glutinosa.…”

Influences of Stock and Scion on Alkaloid Synthesis and Transformation

“…The Knowledge has accumulated as to the role of pectinase in plant disease (1,12,13,14). In this paper evidence will be presented that pectinase preparations can cause a respiratory acceleration and increase of the activities of polyphenol oxidase and peroxidase in host tissues.…”

“…This acetone macerate was theln filtered with suction, and one portion was used for paper clhromiiatography to determine the amounit of nicotine, nornicotine, and anabasine (5, 12), and( another to determine total quantity of alkaloicds by steam distillation and ultraviolet spectrophoitometry (15).…”

Biosynthesis of Anabasine and of Nicotine by Excised Root Cultures of Nicotiana Glauca

“…ISOTOPE ANALYSIS: Samples of the alkaloid picrates were assayed for C14 and for tritium by combustion and proportional gas counting (3,4,5,20 (17). p-Aminobenzoic acid and cyanogen bromide were the spot reagents.…”