Biosynthesis of Anabasine and of Nicotine by Excised Root Cultures of Nicotiana Glauca

Solt, Marie L.; Dawson, Ray F.; Christman, David R.

doi:10.1104/pp.35.6.887

Cited by 70 publications

(18 citation statements)

References 15 publications

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“…The demonstration by Dawson 23 that nicotine 13 is synthesised in roots and transported to the shoots of tobacco plants established this alkaloid as an early target for biosynthetic studies in root cultures. Exploiting sterile root cultures of Nicotiana tabacum and N. glauca, this group performed a series of feeding experiments 24,25 that were definitive in defining nicotinic acid 14 and ornithine 15 as the precursors of 13. The importance of 6 as a precursor of anabasine 16 was also confirmed.…”

Section: Alkaloids 431 Pyrrolidine Alkaloidsmentioning

confidence: 99%

“…The importance of 6 as a precursor of anabasine 16 was also confirmed. 25 Furthermore, it was concluded that synthesis of 13 and 16 does not occur strictly in parallel, even though 14 was proved to be a common precursor. It was also shown that 13 turned over in the cultures, up to 40% of added [ 14 C]-13 being degraded within 38 days.…”

Section: Alkaloids 431 Pyrrolidine Alkaloidsmentioning

confidence: 99%

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The application of root cultures to problems of biological chemistry

Robins¹

1998

Nat. Prod. Rep.

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Introduction 2 The role of root cultures 3 Production, cultivation and properties of root cultures 4 The range of natural products in root cultures 4.1 General aromatic compounds 4.2 Anthraquinones 4.3 Alkaloids 4.3.1 Pyrrolidine alkaloids 4.3.2 Indole alkaloids 4.3.3 Pyrrolizidine alkaloids 4.3.4 Tropane and related alkaloids 4.3.5 Indolizidine alkaloids 4.3.6 Benzylisoquinoline alkaloids 4.4 Terpenoids and steroids 4.4.1 Sesquiterpenes 4.4.2 Diterpenes 4.4.3 Triterpenes and steroids 4.5 Miscellaneous nitrogen compounds 4.6 Miscellaneous sulfur compounds 5 Future prospects 6 References

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Section: Alkaloids 431 Pyrrolidine Alkaloidsmentioning

confidence: 99%

Section: Alkaloids 431 Pyrrolidine Alkaloidsmentioning

confidence: 99%

The application of root cultures to problems of biological chemistry

Robins¹

1998

Nat. Prod. Rep.

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“…The roots are of particular interest as they have been shown to be the site of alkaloid synthesis in many plants of the Solanaceae (5,6). As shown in Atropa, Datura, and Scopolia cell cultures, root differentiation from callus tissue has significantly promoted the synthesis of tropane alkaloids (7,8,9).…”

Section: Introductionmentioning

confidence: 99%

Variation in the Tropane Alkaloid Content ofHyoscyamus muticusPlants and Cell Culture Clones

Oksman-Caldentey¹,

Vuorela²,

Strauss³

et al. 1987

Planta Med

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Systematic studies were carried out on two different strains (Gatersleben and Cairo) of HYOSCYAMUS MUTICUS L. (Solananaceae) in order to analyse the variation in the contents of the two main tropa-alkaloids in individual plants and protoplast-derived cell culture clones. The hyoscyamine content was determined by radioimmunoassay, the scopolamine content by radio- and enzymeimmunoassay and the total tropane alkaloid content by quinuclidinyl benzilate assay. The development stage of the plant is important for alkaloid production. Clear maximal foliar scopolamine and hyoscyamine contents were reached at the onset of flowering and during the full blooming stage, respectively. In the roots the changes in the production of these alkaloids were not considerable. Hyoscyamine is a major alkaloid in the plants as well as in the cell culture clones. In a few exceptions the scopolamine content was greater than that of hyoscyamine, but this phenomenon was not inherited by the cultured clones derived from these plants. High and low tropane alkaloid production was inherited by the selfed F (1), generation plants. Great variability in these alkaloids was observed among individual plants or clones in the same plant or clone population. There was a significant difference between the Cairo strain and the Gatersleben strain as regards their ability to produce tropane alkaloids. Haploid plants of both strains contained more hyoscyamine and scopolamine than the diploid ones. Hyoscyamine and scopolamine were the main alkaloids in the clones and in the plants. Via QNB-assay an interesting clone was found which contains remarkable amounts of unknown tropane-group alkaloids.

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“…6 position was incorporated into nicotine ( Fig. 1, IX) by tobacco roots Dawson et al, 1960;Solt, Dawson, and Christman, 1960). Radioactivity from niacin 2,3, 7_ 14C and quinolinic acid-2, 3, 7, 8-14C was also similarly incorporated (Gholson et al, 1964).…”

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confidence: 97%

Niacin Biosynthesis in Germinating×laeliocattleya Orchid Embryos and Young Seedlings

Arditti

1967

American J of Botany

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The roles of tryptophan, kynurenne, 3‐hydroxyanthranilic acid, and quinolinic acid as niacin precursors were investigated by assessing their germination and growth‐promoting effects in orchid embryo and seedling cultures. Only niacin, 3‐hydroxyanthranilic acid, quinolinic acid and kynurenine exhibited a growth‐promoting effect on seedlings of all ages. Tryptophan inhibited the growth of 70‐day‐old seedlings but enhanced growth of older seedlings. The data presented are interpreted to be consistent with the hypothesis that orchids may synthesize their niacin by a pathway similar to that of certain bacteria, fungi, birds, and mammals.

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Biosynthesis of Anabasine and of Nicotine by Excised Root Cultures of Nicotiana Glauca

Cited by 70 publications

References 15 publications

The application of root cultures to problems of biological chemistry

The application of root cultures to problems of biological chemistry

Variation in the Tropane Alkaloid Content ofHyoscyamus muticusPlants and Cell Culture Clones

Niacin Biosynthesis in Germinating×laeliocattleya Orchid Embryos and Young Seedlings

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