Paper chip-based colorimetric sensing assay for ultra-sensitive detection of residual kanamycin

Ha, Na-Reum; Jung, In-Pil; Kim, Sang‐Heon; Kim, A-Ru; Yoon, Moon‐Young

doi:10.1016/j.procbio.2017.07.008

Cited by 47 publications

(17 citation statements)

References 42 publications

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“…Interestingly, 2 +DOPA showed more intense blue fluorescence compared to native sample‐ 2 (Figure S9a and S9b). This experimental set up may also useful to construct portable chemo‐sensor chip for L–DOPA sensing . The change in the fluorescence of the vesicular solution of 2 in absence and presence of L–DOPA can also be distinguished in the solution state (Figure S9c and S9d).…”

Section: Resultsmentioning

confidence: 99%

Dissipation of Pyrene‐Based Phenylboronic Acid‐Anchored Vesicular Self‐Assemblies: A Motif for Neurotransmitter Recognition

Mandal

Das

2019

ChemistrySelect

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The present article delineates an elegant strategy to develop vesicular self-assembly based bio-molecule recognition motif to sense particular class of neuro-chemicals. Pyrene based phenylboronic acid (PBA) tailored amphiphiles were synthesized that formed vesicular self-assemblies in aqueous solution. Molecular aggregation patterns and various non-covalent forces involved in the vesiculation process were analysed through microscopybased and spectroscopic techniques. The incorporation of PBA functionality in the amphiphilic backbone rendered di-ol sensitivity within the vesicular structures owing to its high affinity to bind reversibly with di-ol (catechol) moieties. Interestingly, the vesicular self-assemblies exhibited enhanced fluorescence signals in response to miniscule amounts of catecholamine-class neurotransmitters (dopamine, adrenaline, and noradrenaline) including L-3,4-dihydroxyphenylalanine (L-DOPA). Moreover, the vesicular aggregates underwent dissipation in presence of L-DOPA. This dissolution of well-ordered molecular arrangement along with fluorescence enhancement took place due to the probable formation of boronate-diol adduct. The soft materials failed to exhibit any physicochemical change in the presence of other neuro-chemicals devoid of a catechol moiety. Thus, the di-ol induced dissipation and fluorescent augmentation feature of the PBA tethered vesicular aggregates hoisted the soft-material as proficient biomarker for catecholamine class neurotransmitters.[a] Dr.

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Section: Resultsmentioning

confidence: 99%

Dissipation of Pyrene‐Based Phenylboronic Acid‐Anchored Vesicular Self‐Assemblies: A Motif for Neurotransmitter Recognition

Mandal

Das

2019

ChemistrySelect

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“…There are several reports for kanamycin detection using different colorimetric-based biosensing assays, due to its low cost, simplicity, and observation of the color change by the naked eye [ 32 , 74 , 75 , 76 , 77 , 78 , 79 ]. However, among them, silver nanoparticles (AgNPs) and AuNP-based colorimetric aptasensors have attracted more and more attention due to size/distance-dependent optical properties [ 76 , 77 ].…”

Section: Aptasensors For Different Antibiotic Classesmentioning

confidence: 99%

“…However, among them, silver nanoparticles (AgNPs) and AuNP-based colorimetric aptasensors have attracted more and more attention due to size/distance-dependent optical properties [ 76 , 77 ]. Thus, different research groups have tried to design a sensitive and specific kanamycin aptasensor based on AgNPs and AuNPs for the possible application in food or clinical samples [ 76 , 77 , 78 , 79 ].…”

Section: Aptasensors For Different Antibiotic Classesmentioning

confidence: 99%

Aptamer-Based Biosensors for Antibiotic Detection: A Review

2018

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Antibiotic resistance and, accordingly, their pollution because of uncontrolled usage has emerged as a serious problem in recent years. Hence, there is an increased demand to develop robust, easy, and sensitive methods for rapid evaluation of antibiotics and their residues. Among different analytical methods, the aptamer-based biosensors (aptasensors) have attracted considerable attention because of good selectivity, specificity, and sensitivity. This review gives an overview about recently-developed aptasensors for antibiotic detection. The use of various aptamer assays to determine different groups of antibiotics, like β-lactams, aminoglycosides, anthracyclines, chloramphenicol, (fluoro)quinolones, lincosamide, tetracyclines, and sulfonamides are presented in this paper.

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“…The gold nanoparticles used in this paper is same as it used in prior research [30,31]. Gold nanoparticle was prepared from citrate reduction of HAuCl 4 .…”

Section: Gold Nanoparticle Synthesismentioning

confidence: 99%

Detection of Nonylphenol with a Gold-Nanoparticle-Based Small-Molecule Sensing System Using an ssDNA Aptamer

Kim

et al. 2019

IJMS

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Endocrine-disrupting chemicals (EDCs) threaten many kinds of life throughout the world. These compounds function the same as sexual hormones, inducing precocious puberty, gynecomastia, etc., in the human body. To prevent excess exposure to nonylphenol (NP), a simple and rapid detection system is needed. In this study, we develop a nonylphenol-specific aptamer from a random single-stranded DNA library and test a rapid sensor system based on the aptamer and gold nanoparticles (AuNPs). The aptamer was screened by a methodology involving reduced graphene oxide (rGO). As a result of screening and sequencing, a DNA aptamer was developed that recognizes the target with high binding affinity (Kd = 194.2 ± 65.9 nM) and specificity. The sensor system developed using the aptamer and gold nanoparticles is sensitive (LOD = 2.239 nM). Circular dichroism (CD) spectrometry results show that the free aptamer binds to the target molecule. The aptamer was characterized using gold nanoparticles to measure UV absorbance. Our results suggest that the sensor system developed using this aptamer is useful for field diagnosis of small molecules.

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Paper chip-based colorimetric sensing assay for ultra-sensitive detection of residual kanamycin

Cited by 47 publications

References 42 publications

Dissipation of Pyrene‐Based Phenylboronic Acid‐Anchored Vesicular Self‐Assemblies: A Motif for Neurotransmitter Recognition

Dissipation of Pyrene‐Based Phenylboronic Acid‐Anchored Vesicular Self‐Assemblies: A Motif for Neurotransmitter Recognition

Aptamer-Based Biosensors for Antibiotic Detection: A Review

Detection of Nonylphenol with a Gold-Nanoparticle-Based Small-Molecule Sensing System Using an ssDNA Aptamer

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