Paper‐Based Ratiometric Fluorescence Analytical Devices towards Point‐of‐Care Testing of Human Serum Albumin

Abstract

Monitoring of human serum albumin (HSA) in a point‐of‐care fashion is urgently needed in particular for elderly or chronically ill patients. Herein, a dual‐state emissive chalcone probe having the feature of aggregation‐induced emission was designed and synthesized. The concentration of HSA can be evaluated by the ratios of emission from probes in aggregated and monomeric state, which gives a visually discernible red‐to‐green color change. A simple, portable paper‐based analytical device have been fabricated by integration of the recognition probe in the detection pad and employed for HSA test using the whole blood samples. This paper‐based assay shows the analytical capability comparable to the standard testing methods but is in a point‐of‐care fashion, providing a promising tool for at‐home HSA detection and HSA‐related disease diagnosis.

Grand Challenges in Biosensors and Biomolecular Electronics

“…Current advances in paper-based microfluidic analytical devices, and mobile-phone based diagnosis have greatly contributed to this aspect. A disposable paper-based test strip can significantly low the detection cost and make it invasive by using a body fluid such as saliva or urine (Luo et al, 2020). By integration with personal equipment such as a mobile phone or a glucose test meter, selfquantitative detection can be performed by end-users without visiting the hospital.…”

NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel

“…Therefore, the hERG potassium channel may be the target for particular cancer treatment. To better analyze and determine the hERG potassium channel at the molecular and cellular level, developing a simple, cost-effective, and safe fluorescent method for labeling the hERG potassium channel is urgently required (Luo et al, 2020). Effective development of such fluorescent labeling may explain the hERG channel's role in those cancers and anatomical locations, thus establishing the hERG channel as a brand new target and/or biomarker for antitumor agents.…”

Gradients of a Sandwich-structured Immunosensor Controlled Using Magnetized Masks for Determination of Human Serum Albumin Using Electrochemiluminescence

“…Other reports with uorescence spectrometry to determine HSA level are still unsatis ed in selectivity and stability due to limit of uorescent resolution. Otherwise, these approaches involving complicated synthesis routes of these immunoassays are di cult to commercialize [31][32][33]. Therefore, handy immunosensors that can be synthesized simply to detect HSA conveniently with excellent performance are desirable.…”