Paper-based loop-mediated isothermal amplification and lateral flow (LAMP-LF) assay for identification of tissues of cattle origin

Jawla, Jyoti; Kumar, Rajiv; Mendiratta, S. K.; Agarwal, Rajesh; Kumari, Sarita; Saxena, Vikas; Kumar, Dinesh; Singh, Praveen; Boby, Nongthombam; Rana, Preeti

doi:10.1016/j.aca.2021.338220

Cited by 31 publications

(13 citation statements)

References 32 publications

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“…For instance, to enhance the detection limitation and achieve absolute DNA quantitation, droplet digital PCR could be employed, which could be appropriate for the identification of highly processed Chinese medicine or Chinese Patent Medicine. Furthermore, to achieve efficient on-site identification of commercial Chinese medicine products, visual and more rapid detection methods could be developed, such as loop-mediated isothermal amplification (Jawla et al, 2021; Zhao et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Development and Application of qPCR Assay for Species-specific Identification of Two Animal-derived Tonics: Vinegar-quenched Testudinis Carapax et Plastrum and Vinegar-quenched Trionycis Carapax

Chen

Han

et al. 2023

Pharmacognosy Magazine

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Background Vinegar-quenched Testudinis Carapax et Plastrum (TCP) and vinegar-quenched Trionycis Carapax (TC) are made from the shells of Chinemys reevesii and Trionyx sinensis. However, some cheap substitutes from non-medicinal species such as Trachemys scripta, Mauremys sinensis, Chelydra serpentina, and Apalone ferox are often seen on the market, which exposes public health to high risk. Conventional methods are not applicable for the identification of these two products since their intrinsic constituents are largely destroyed after being highly processed. Objectives A new quantitative polymerase chain reaction (qPCR) method was developed to detect DNA components of Vinegar-quenched TCP and vinegar-quenched TC based on simple sequence repeat differential genes of animal-derived mitochondria. Materials and Methods Six species-specific primer reactions were designed and further validated for specificity, sensitivity, and repeatability. Finally, the developed method was used to assess the authenticity of their commercial products. Results The assay exhibited good specificity, and the limit of detection was above 1 × 102 copies/µL. There was good linearity in the concentration, ranging from 1 × 102 to 1 × 107 copies/µL. In addition, the method was validated through repeatability assessment (coefficient variation <1%). While qPCR was applied for the analysis of 36 batches of commercial products of these two tonics, it could effectively identify the authenticity of the products, and consequently, seven batches were fake or adulterated. Conclusion This newly proposed method is promising for the quality evaluation of highly processed animal-derived Chinese medicines, which will assist in ensuring safety and efficacy in clinical practice and protect fair trade in the market.

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Section: Discussionmentioning

confidence: 99%

Development and Application of qPCR Assay for Species-specific Identification of Two Animal-derived Tonics: Vinegar-quenched Testudinis Carapax et Plastrum and Vinegar-quenched Trionycis Carapax

Chen

Han

et al. 2023

Pharmacognosy Magazine

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“…The whole detection process could be completed within 20 min. Interestingly, the colorimetric LFA platforms have been successfully integrated with DNA amplification approaches such as LAMP [143][144][145][146] and CRISPR. [147][148][149][150] Another remarkable design that has been successfully developed is an origami paper-based analytical device (o-PAD).…”

Section: Colorimetric Detectionmentioning

confidence: 99%

Section: Detection Techniquesmentioning

confidence: 99%

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

Mahardika,

Naorungroj,

Khamcharoen

et al. 2023

Advanced NanoBiomed Research

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DNA chips play a crucial role in point‐of‐care diagnostics by enabling rapid and accurate detection of genetic information. These chips offer high sensitivity and selectivity, allowing for the identification of specific DNA sequences associated with diseases and pathogens. Integration into lab‐on‐chip platforms streamlines and miniaturizes diagnostic workflows, paving the way for cost‐effective, portable, and user‐friendly testing devices that can revolutionize healthcare delivery. In this review, a comprehensive description of the platforms utilized in DNA analysis, including microfluidic devices and integrated DNA chips, is provided. It explores the selection and immobilization of DNA probes for improved selectivity. Additionally, it covers diverse detection techniques such as optical detection (colorimetry and fluorescence) and electrochemical techniques. In these discussions, it is aimed to provide a thorough understanding of the current state of the art in DNA biosensor‐integrated lab‐on‐chip technology for point‐of‐care testing. The continued advancements in DNA chip technology hold immense promise for the development of next‐generation point‐of‐care diagnostics, where integrated sample preparation and rapid results generation can further enhance patient outcomes and contribute to the effective management of diseases.

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“…This is one of the significant features of our device because most reported onchip LAMP methods did not present quantitative analysis. 41,[66][67][68] We evaluated the calibration curve, detection sensitivity, and LOD of our device to detect N. meningitidis by testing a series of 10-fold diluted initial template DNA samples (i.e., 6 × 10 6 , 6 × 10 5 , 6 × 10 4 , 6 × 10 3 , 6 × 10 2 , 6 × 10 1 , 6 × 10 0 , and 6 × 10 −1 DNA copies per LAMP zone before LAMP amplification) on this microfluidic device after 1 h LAMP amplification reaction. The recovered fluorescence intensities corresponding to different copy numbers of the initial template DNA were achieved from various nanobiosensor detection zones to obtain the calibration curve.…”

Section: Analytical Performance Of the μFpad For Quantitative Detectionmentioning

confidence: 99%

A microfluidic fully paper-based analytical device integrated with loop-mediated isothermal amplification and nano-biosensors for rapid, sensitive, and specific quantitative detection of infectious diseases

et al. 2022

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Paper-based loop-mediated isothermal amplification and lateral flow (LAMP-LF) assay for identification of tissues of cattle origin

Cited by 31 publications

References 32 publications

Development and Application of qPCR Assay for Species-specific Identification of Two Animal-derived Tonics: Vinegar-quenched Testudinis Carapax et Plastrum and Vinegar-quenched Trionycis Carapax

Development and Application of qPCR Assay for Species-specific Identification of Two Animal-derived Tonics: Vinegar-quenched Testudinis Carapax et Plastrum and Vinegar-quenched Trionycis Carapax

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

A microfluidic fully paper-based analytical device integrated with loop-mediated isothermal amplification and nano-biosensors for rapid, sensitive, and specific quantitative detection of infectious diseases

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