Paper‐Based ELISA

Cheng, Chao‐Min; Martinez, Andres W.; Gong, Jinlong; Mace, Charles R.; Phillips, Scott T.; Carrilho, Emanuel; Mirica, Katherine A.; Whitesides, George M.

doi:10.1002/anie.201001005

Cited by 633 publications

(461 citation statements)

References 23 publications

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Section: Microfluidic Devicesmentioning

confidence: 99%

“…Microfluidic paper-based analytical devices (μPADs) was pioneered by Dr. White sides group [18]. Application of paper-based microfluidic platforms includes, human health diagnosis, nucleic acid analysis, cellular analysis, and food safety analysis [18]. Different approaches have been used for the detection using paper-based devices including colorimetric detection, chemiluminescence detection, electrochemical detection, and fluorescence detection [19].…”

Section: Microfluidic Devicesmentioning

confidence: 99%

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Microfluidic Platforms for the Detection of Disease Biomarkers

Chalise

Timilsina

2017

IJBSBE

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Biomarkers, which can be obtained from different sources including blood, tissues, body fluids, and urine, can be evaluated to indicate normal, pathogenic, or therapeutic response to biological system. Early detection of biomarkers not only prevent the spread of infectious disease but also drastically decrease the death rate of people suffering from different diseases, especially in rural areas where financial resources are limited. Low cost microfluidic technologies have applications in human health diagnosis, food safety, and environmental analysis. This article briefly reviews different kinds of microfluidic platforms including paper, polymer, and hybrid platforms as a microfluidic biosensor for the detection of disease biomarkers. It also discusses the current limitations and future prospects. Keywords:

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Section: Microfluidic Devicesmentioning

confidence: 99%

Section: Microfluidic Devicesmentioning

confidence: 99%

Microfluidic Platforms for the Detection of Disease Biomarkers

Chalise

Timilsina

2017

IJBSBE

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“…[20][21][22][23][24] Such multiple reactions and washing need to be carried out independently of the respective reaction, and contamination needs to be avoided. Conventional immunoassays using 96-well microplates completely replace the solution with every reaction and wash, but complicated steps and know-how are required for paper substrates.…”

Section: Introductionmentioning

confidence: 99%

Practical High-Performance Lateral Flow Assay Based on Autonomous Microfluidic Replacement on a Film

2018

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Although paper-based microfluidic devices are an ideal platform for point-of-care (POC) diagnostics, it is difficult to achieve microfluidic control required for sensitive analyses such as ELISA on a paper substrate. Here, we present a novel lateral-flow test chip that can perform operations similar to a pump, such as flowing, stopping, and replacing a solution, just by adding the solution onto an inlet port. The chip was fabricated by laminating paper, film, and adhesive tape. For sensitive and accurate detection in an immunoassay, the transparency and flatness of the substrate is crucial for precise analysis of weak light generated by a specific antigen-antibody reaction; however, paper is not flat and is opaque. Therefore, transparent film was applied to the detection area of the chip in this study. The chip showed a good correlation at 0.1 -100 ng ml -1 concentrations of C-reactive protein, demonstrating high quantitative analysis of CRP in serum suitable for clinical trials. The signal intensity of the novel chip was higher than that of a chip made of nitrocellulose membrane, and the variation was smaller. The limit of detection of the chip was 0.1 ng ml -1 , whereas that of the nitrocellulose membrane was 100 ng ml -1 . This novel chip can be used for sensitive sandwich immunoassays just by adding solutions.

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“…(4) They demonstrated various biosensing techniques, for example, paper-based enzyme-linked immunosorbent assay (ELISA) and paper-based electrochemical glucose sensing, which can be accomplished with the commercialized glucose sensor. (5,6) μPADs have attracted attention not only for human diagnosis but also for use in agricultural fields and environmental measurement because of their very low cost measurement and easy fabrication methods. Therefore, μPADs have been studied by several groups in the last decade.…”

Section: Introductionmentioning

confidence: 99%

Effects of Electrode Placement and Measurement Time on Electrochemical Signal of Redox Species Flowing through Porous Material

2016

Sensors and Materials

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We have studied the construction of an electrochemical immunochromatography platform as one of the point-of-care testing devices from both experimental and theoretical aspects. In the electrochemical immunochromatography measurement, the layout of working, reference, and counter electrodes and the flow rate in a porous membrane are important in obtaining a highly sensitive and stable electrochemical signal. In this manuscript, we report the effects of the layout of the three electrodes and the elapsed time after sample injection on electrochemical signals. We found that the working electrode should be placed upstream of the reference electrode because an unfavorable electrochemical signal from a Ag + ion from the reference electrode was observed on the working electrode when it was located downstream of the reference electrode. Furthermore, we found that the electrochemical signal was inversely proportional to the sixth root of time. Experimental and theoretical results are consistent with each other, exhibiting a high correlation coefficient of 0.99.

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Paper‐Based ELISA

Abstract: Paper works: Paper‐based indirect ELISA (see picture) has been demonstrated through the detection of rabbit IgG and the HIV‐1 envelope antigen gp41. This technique combines the sensitivity and specificity of ELISA with the low cost and ease‐of‐use of paper‐based platforms.

Cited by 633 publications

References 23 publications

Microfluidic Platforms for the Detection of Disease Biomarkers

Microfluidic Platforms for the Detection of Disease Biomarkers

Practical High-Performance Lateral Flow Assay Based on Autonomous Microfluidic Replacement on a Film

Effects of Electrode Placement and Measurement Time on Electrochemical Signal of Redox Species Flowing through Porous Material

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