Paper-based electroanalytical sensing platforms

Metters, Jonathan P.; Houssein, Said M.; Kampouris, Dimitrios K.; Banks, Craig E.

doi:10.1039/c2ay26396c

Cited by 86 publications

(44 citation statements)

References 34 publications

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“…On the contrary, no anodic process was found for NH 3 , whose oxidation was clearly shifted beyond the solvent discharge. This was not surprising, since a marked overpotential increase is frequently encountered for several electrochemical processes when glassy carbon surfaces are replaced by carbon‐paste‐type materials as carbon inks 45, 46. In particular, such an anodic shift was observed previously just for ammonia 47.…”

Section: Resultsmentioning

confidence: 72%

Structural and Kinetic Dissection of the endo‐α‐1,2‐Mannanase Activity of Bacterial GH99 Glycoside Hydrolases from Bacteroides spp.

Hakki

Thompson

Bellmaine

et al. 2014

Chemistry A European J

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Glycoside hydrolase family 99 (GH99) was created to categorize sequence-related glycosidases possessing endo-α-mannosidase activity: the cleavage of mannosidic linkages within eukaryotic N-glycan precursors (Glc1-3 Man9 GlcNAc2 ), releasing mono-, di- and triglucosylated-mannose (Glc1-3 -1,3-Man). GH99 family members have recently been implicated in the ability of Bacteroides spp., present within the gut microbiota, to metabolize fungal cell wall α-mannans, releasing α-1,3-mannobiose by hydrolysing αMan-1,3-αMan→1,2-αMan-1,2-αMan sequences within branches off the main α-1,6-mannan backbone. We report the development of a series of substrates and inhibitors, which we use to kinetically and structurally characterise this novel endo-α-1,2-mannanase activity of bacterial GH99 enzymes from Bacteroides thetaiotaomicron and xylanisolvens. These data reveal an approximate 5 kJ mol(-1) preference for mannose-configured substrates in the -2 subsite (relative to glucose), which inspired the development of a new inhibitor, α-mannopyranosyl-1,3-isofagomine (ManIFG), the most potent (bacterial) GH99 inhibitor reported to date. X-ray structures of ManIFG or a substrate in complex with wild-type or inactive mutants, respectively, of B. xylanisolvens GH99 reveal the structural basis for binding to D-mannose- rather than D-glucose-configured substrates.

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Section: Resultsmentioning

confidence: 72%

Structural and Kinetic Dissection of the endo‐α‐1,2‐Mannanase Activity of Bacterial GH99 Glycoside Hydrolases from Bacteroides spp.

Hakki

Thompson

Bellmaine

et al. 2014

Chemistry A European J

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“…This electrochemical probe receives considerable attention owing to its very important role as a cofactor in many naturally occurring enzymatic reactions, and mainly because of the potential application in over 300 NAD + /NADH À dependent dehydrogenase-based biosensors [21][22][23][24][25][26][27] and consequently its electrochemical characteristics are hugely important. The kinetic parameter, 4 is tabulated as a function of peak-to-peak separation (DE P ) at a set temperature (298 K) for a one-step, one electron process.…”

Section: Resultsmentioning

confidence: 99%

Exploring the electrical wiring of screen-printed configurations utilised in electroanalysis

et al. 2015

Self Cite

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In this technical note the often overlooked issue of electrically connecting screen-printed electrode sensors is considered. The electrical connection of screen-printed electrodes to the potentiostat/ electronics can be a main issue when a true electrochemical response is trying to be obtained and if one does not have a stable electrical connection, the results that are obtained may lack reproducibility and therefore maybe discredited as "another failed electrochemical probe/analyte". This paper considers the case of electrically connecting screen-printed with crocodile clips and compares that to the use of edge-connectors demonstrating the precise connection of an electrode setup when utilising crocodile clips can result in extremely reproducible electrochemical outputs when applied correctly.

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“…Fig. 3A shows a scanning electron micrograph of the electrode surface, revealing typical graphite ink morphology with a rough surface over the polyester sheet [17,18]. Vertical inspection by optical microscopy (Fig.…”

Section: Dcell Fabrication and Electrochemical Characterizationmentioning

confidence: 99%

Simple and rapid fabrication of disposable carbon-based electrochemical cells using an electronic craft cutter for sensor and biosensor applications

Afonso

Uliana

Martucci

et al. 2016

Talanta

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Paper-based electroanalytical sensing platforms

Cited by 86 publications

References 34 publications

Structural and Kinetic Dissection of the endo‐α‐1,2‐Mannanase Activity of Bacterial GH99 Glycoside Hydrolases from Bacteroides spp.

Structural and Kinetic Dissection of the endo‐α‐1,2‐Mannanase Activity of Bacterial GH99 Glycoside Hydrolases from Bacteroides spp.

Exploring the electrical wiring of screen-printed configurations utilised in electroanalysis

Simple and rapid fabrication of disposable carbon-based electrochemical cells using an electronic craft cutter for sensor and biosensor applications

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