Simple and rapid fabrication of disposable carbon-based electrochemical cells using an electronic craft cutter for sensor and biosensor applications

Afonso, André S.; Uliana, Carolina Venturini; Martucci, Diego H.; Faria, Ronaldo C.

doi:10.1016/j.talanta.2015.09.002

Cited by 64 publications

(42 citation statements)

References 37 publications

(32 reference statements)

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“…Screen‐printing technology is employed by many research groups for the fabrication of electrochemical sensors due to their satisfactory sensitivities, possibility of miniaturization, portability and effective cost of the devices , . These sensors aim as main characteristics the ease of fabrication over low‐cost and good reproducibility , , .…”

Section: Introductionsupporting

confidence: 80%

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Simple and Inexpensive Electrochemical Platform Based on Novel Homemade Carbon Ink and its Analytical Application for Determination of Nitrite

2017

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In this work we present the development of a simple handmade approach for the easy fabrication of three‐electrode electrochemical devices based on newly in‐house developed carbon ink composed of graphite powder and polystyrene. Different proportions of graphite/polystyrene were investigated for the optimization of the ink. The counter and reference electrodes were produced using commercial carbon ink and silver glue. Scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry were used to investigate the morphology and the electrochemical properties of the sensor. The results showed that the electroactive area of the optimized working electrode was ca. 2.35 times larger than its geometric area. The RSD values obtained for repeatability and reproducibility were 0.20% and 2.78%, respectively, which suggest no significant variation on the electrodes fabricated. The analytical feasibility of the electrode was tested through its application for the determination of nitrite in drinking water. The quantifications were successfully performed at levels below the maximum contaminant level established for nitrite. A limit of detection of 1.42 × 10−6 mol L−1 and recoveries of ca. 103 % were achieved. The results were validated using ion‐chromatography technique with good agreement. The performance of the unmodified sensor proposed here on nitrite determination was better than some recently reported modified electrodes obtained through complex procedures.

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Section: Introductionsupporting

confidence: 80%

“…However the most of these materials require complicated chemical synthesis steps to be obtained. Among the substrates used to assemble the SPEs are polyester transparency film , , , polypropylene film , polyethylene terephthalate (PET) , polyimide and ceramic . Commercial carbon inks are frequently utilized to produce the electrodes.…”

Section: Introductionmentioning

confidence: 99%

Simple and Inexpensive Electrochemical Platform Based on Novel Homemade Carbon Ink and its Analytical Application for Determination of Nitrite

2017

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“…The sensor electrodes were fabricated by hand screen printing carbon graphitic ink using a patterned adhesive-backed vinyl mask template (Afonso et al, 2015 in press). First, a mask template was designed using AutoCAD and converted to compatible format for cutting, then a vinyl mask was cut using a portable precision desktop cutter (Cameo®, Silhouette America, Inc.).…”

Section: Methodsmentioning

confidence: 99%

3D-printed supercapacitor-powered electrochemiluminescent protein immunoarray

Kadimisetty

Mosa

Malla

et al. 2016

Biosensors and Bioelectronics

Self Cite

157

148

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Herein we report a low cost, sensitive, supercapacitor-powered electrochemiluminescent (ECL) protein immunoarray fabricated by an inexpensive 3-dimensional (3D) printer. The immunosensor detects three cancer biomarker proteins in serum within 35 min. The 3D-printed device employs hand screen printed carbon sensors with gravity flow for sample/reagent delivery and washing. Prostate cancer biomarker proteins, prostate specific antigen (PSA), prostate specific membrane antigen (PSMA) and platelet factor-4 (PF-4) in serum were captured on the antibody-coated carbon sensors followed by delivery of detection-antibody-coated Ru(bpy)32+ (RuBPY)-doped silica nanoparticles in a sandwich immunoassay. ECL light was initiated from RuBPY in the silica nanoparticles by electrochemical oxidation with tripropylamine (TPrA) co-reactant using supercapacitor power and ECL was captured with a CCD camera. The supercapacitor was rapidly photo-recharged between assays using an inexpensive solar cell. Detection limits were 300–500 fg mL−1 for the 3 proteins in undiluted calf serum. Assays of 6 prostate cancer patient serum samples gave good correlation with conventional single protein ELISAs. This technology could provide sensitive onsite cancer diagnostic tests in resource-limited settings with the need for only moderate-level training.

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“…Carbon-based electrodes (e.g., graphite, graphene, CNTs), such as those shown in Fig. 7a (Afonso et al 2016) and 7b , are now being examined as potential alternatives to relatively more expensive metallic or ceramic electrodes. Many of these carbon-based materials are also nanoscale in structure, and thus offer advantages regarding nanostructuring.…”

Section: Emerging Electrode Materials Fabrication Processes and Formentioning

confidence: 99%

“…Similarly, polymer-based electrodes have also been examined as low-cost alternatives to metal electrodes as described in Section 2.1.3. For example, Afonso et al used a home craft cutter printer as a highly accessible means of fabricating high quantities of disposable carbon-based sensors (Afonso et al 2016).…”

Section: Emerging Electrode Materials Fabrication Processes and Formentioning

confidence: 99%

Electrochemical biosensors for pathogen detection

Cesewski

Johnson

2020

Biosensors and Bioelectronics

608

358

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A B S T R A C TRecent advances in electrochemical biosensors for pathogen detection are reviewed. Electrochemical biosensors for pathogen detection are broadly reviewed in terms of transduction elements, biorecognition elements, electrochemical techniques, and biosensor performance. Transduction elements are discussed in terms of electrode material and form factor. Biorecognition elements for pathogen detection, including antibodies, aptamers, and imprinted polymers, are discussed in terms of availability, production, and immobilization approach. Emerging areas of electrochemical biosensor design are reviewed, including electrode modification and transducer integration. Measurement formats for pathogen detection are classified in terms of sample preparation and secondary binding steps. Applications of electrochemical biosensors for the detection of pathogens in food and water safety, medical diagnostics, environmental monitoring, and bio-threat applications are highlighted. Future directions and challenges of electrochemical biosensors for pathogen detection are discussed, including wearable and conformal biosensors, detection of plant pathogens, multiplexed detection, reusable biosensors for process monitoring applications, and low-cost, disposable biosensors.

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Simple and rapid fabrication of disposable carbon-based electrochemical cells using an electronic craft cutter for sensor and biosensor applications

Cited by 64 publications

References 37 publications

Simple and Inexpensive Electrochemical Platform Based on Novel Homemade Carbon Ink and its Analytical Application for Determination of Nitrite

Simple and Inexpensive Electrochemical Platform Based on Novel Homemade Carbon Ink and its Analytical Application for Determination of Nitrite

3D-printed supercapacitor-powered electrochemiluminescent protein immunoarray

Electrochemical biosensors for pathogen detection

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