Paper-based bioactive scaffolds for stem cell-mediated bone tissue engineering

Park, Hyun Ji; Yu, Seung Jung; Yang, Kisuk; Jin, Yoonhee; Cho, Ann Na; Kim, Jin; Lee, Bora; Yang, Hee Seok; Im, Sung Gap; Cho, Seung Woo

doi:10.1016/j.biomaterials.2014.09.002

Cited by 98 publications

(97 citation statements)

References 54 publications

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“…22 . The scaffolds were fixed with 3.8% (w/v) formaldehyde (Sigma aldrich, St. Louis, MO, USA) in phosphate-buffered saline (PBS) for 20 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues

Kim

Yun

Kim

2017

Sci Rep

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Cell-laden scaffolds are widely investigated in tissue engineering because they can provide homogenous cell distribution after long culture periods, and deposit multiple types of cells into a designed region. However, producing a bioceramic 3D cell-laden scaffold is difficult because of the low processability of cell-loaded bioceramics. Therefore, designing a 3D bioceramic cell-laden scaffold is important for ceramic-based tissue regeneration. Here, we propose a new strategy to fabricate an alpha-tricalcium-phosphate (α-TCP)/collagen cell-laden scaffold, using preosteoblasts (MC3T3-E1), in which the volume fraction of the ceramic exceeded 70% and was fabricated using a two-step printing process. To fabricate a multi-layered cell-laden scaffold, we manipulated processing parameters, such as the diameter of the printing nozzle, pneumatic pressure, and volume fraction of α-TCP, to attain a stable processing region. A cell-laden pure collagen scaffold and an α-TCP/collagen scaffold loaded with cells via a simple dipping method were used as controls. Their pore geometry was similar to that of the experimental scaffold. Physical properties and bioactivities showed that the designed scaffold demonstrated significantly higher cellular activities, including metabolic activity and mineralization, compared with those of the controls. Our results indicate that the proposed cell-laden ceramic scaffold can potentially be used for bone regeneration.

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“…22 . The scaffolds were fixed with 3.8% (w/v) formaldehyde (Sigma aldrich, St. Louis, MO, USA) in phosphate-buffered saline (PBS) for 20 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues

Kim

Yun

Kim

2017

Sci Rep

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“…43 Statistical analysis was performed using the unpaired Student's t-test using GraphPad Prism 6 software (Graphpad Software, San Diego, CA, USA). A p-value smaller than 0.05 was considered statistically significant.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Thermo-responsive polymeric nanoparticles for enhancing neuronal differentiation of human induced pluripotent stem cells

Seo

Cho

Jang

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

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“…[ 13 ] In general, the papersupported cell culture is easy to assemble, inexpensive for one-time use, compatible with high throughputs, and amenable to rapid analysis. [ 14 ] Despite these comparative perspectives, the extensive use of the paper-supported cell culture is still challenged. There exist two drawbacks from the paper-supported scaffolds: (1) the fi bers of the chromatography paper provide mechanical support, but not good enough for cell adhesion onto its surface; and (2) the fi bers of the chromatography paper scatter light, thus limiting the fl uorescence imaging capabilities.…”

mentioning

confidence: 99%

Engineering Cell‐Compatible Paper Chips for Cell Culturing, Drug Screening, and Mass Spectrometric Sensing

Chen

Liu

et al. 2015

Adv Healthcare Materials

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Paper-supported cell culture is an unprecedented development for advanced bioassays. This study reports a strategy for in vitro engineering of cell-compatible paper chips that allow for adherent cell culture, quantitative assessment of drug efficiency, and label-free sensing of intracellular molecules via paper spray mass spectrometry. The polycarbonate paper is employed as an excellent alternative bioscaffold for cell distribution, adhesion, and growth, as well as allowing for fluorescence imaging without light scattering. The cell-cultured paper chips are thus amenable to fabricate 3D tissue construction and cocultures by flexible deformation, stacks and assembly by layers of cells. As a result, the successful development of cell-compatible paper chips subsequently offers a uniquely flexible approach for in situ sensing of live cell components by paper spray mass spectrometry, allowing profiling the cellular lipids and quantitative measurement of drug metabolism with minimum sample pretreatment. Consequently, the developed paper chips for adherent cell culture are inexpensive for one-time use, compatible with high throughputs, and amenable to label-free and rapid analysis.

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Paper-based bioactive scaffolds for stem cell-mediated bone tissue engineering

Cited by 98 publications

References 54 publications

An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues

An innovative cell-laden α-TCP/collagen scaffold fabricated using a two-step printing process for potential application in regenerating hard tissues

Thermo-responsive polymeric nanoparticles for enhancing neuronal differentiation of human induced pluripotent stem cells

Engineering Cell‐Compatible Paper Chips for Cell Culturing, Drug Screening, and Mass Spectrometric Sensing

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