Paper-based analytical device with colorimetric detection for urease activity determination in soils and evaluation of potential inhibitors

Tavares, Maria Célia; Oliveira, Karoliny Almeida; Fátima, Ângelo de; Coltro, Wendell K. T.; Santos, Josué Carinhanha Caldas

doi:10.1016/j.talanta.2021.122301

Cited by 24 publications

(13 citation statements)

References 64 publications

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“…The soybean solution was centrifuged at a rate of 10,000 rpm/min for 30 min at 25 • C. Finally, the supernatant (crude urease) was collected and then lyophilized using FreeZone 6 Liter Benchtop Freeze Dry Systems with Stoppering Tray Dryers, Labconco. The activity of urease was tested using an acid/base indicator (phenol red) to monitor the enzymatic activities for colorimetric assay [32,33]. The 2.5 µg/µL urease and 2% w/v urea solution were added to 0.05 mM phenol red as shown in Figure S5.…”

Section: The Extraction Of Ureasementioning

confidence: 99%

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A Disposable Electrochemical Biosensor Based on Screen-Printed Carbon Electrodes Modified with Silver Nanowires/HPMC/Chitosan/Urease for the Detection of Mercury (II) in Water

et al. 2021

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This work describes the facile preparation of a disposable electrochemical biosensor for the detection of Hg(II) in water by modifying the surface of a screen-printed carbon electrode (SPCE). The surface modification consists of the immobilization of a composite layer of silver nanowires, hydroxymethyl propyl cellulose, chitosan, and urease (AgNWs/HPMC/CS/Urease). The presence of the composite was confirmed by scanning electron microscopy (SEM) and its excellent conductivity, due chiefly to the electrical properties of silver nanowires, enhanced the sensitivity of the biosensor. Under optimum conditions, the modified SPCE biosensor showed excellent performance for the detection of Hg(II) ions, with an incubation time of 10 min and a linear sensitivity range of 5–25 µM. The limit of detection (LOD) and limit of quantitation (LOQ) were observed to be 3.94 µM and 6.50 µM, respectively. In addition, the disposable and portable biosensor exhibited excellent recoveries for the detection of Hg(II) ions in commercial drinking water samples (101.62–105.26%). The results are correlated with those obtained from inductively coupled plasma optical emission spectrometry (ICP-OES), indicating that our developed sensor is a reliable method for detection of Hg(II) in real water samples. The developed sensor device is a simple, effective, portable, low cost, and user-friendly platform for real-time detection of heavy metal ions in field measurements with potential for other biomedical applications in the future.

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Section: The Extraction Of Ureasementioning

confidence: 99%

“…The 2.5 µg/µL urease and 2% w/v urea solution were added to 0.05 mM phenol red as shown in Figure S5. The color of the phenol red solution was changed from yellow to pink, indicating that ammonium species were obtained as the final product [33].…”

Section: The Extraction Of Ureasementioning

confidence: 99%

A Disposable Electrochemical Biosensor Based on Screen-Printed Carbon Electrodes Modified with Silver Nanowires/HPMC/Chitosan/Urease for the Detection of Mercury (II) in Water

et al. 2021

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“…Urease is a nickel-dependent enzyme found in many organisms, including fungi, algae, plants, and prokaryotes. It is very substrate-specific, and catalyzes the hydrolysis of urea into carbon dioxide and ammonia [ 54 ]. Because urease is a biologically relevant compound in natural organisms [ 55 ], we used it as the ligand to bind nickel with voltametric measurements.…”

Section: Resultsmentioning

confidence: 99%

Development, Optimization, Characterization, and Application of Electrochemical Biosensors for Detecting Nickel Ions in Food

et al. 2021

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Nickel is naturally present in drinking water and many dietary items, which expose the general population to nickel ingestion. This heavy metal can have a variety of harmful health effects, causing allergies and skin disorders (i.e., dermatitis), lung, cardiovascular, and kidney diseases, and even certain cancers; therefore, nickel detection is important for public health. Recent innovations in the development of biosensors have demonstrated they offer a powerful new approach over conventional analytical techniques for the identification and quantification of user-defined compounds, including heavy metals such as nickel. We optimized five candidate nickel-biosensing receptors, and tested each for efficiency of binding to immobilization elements on screen-printed electrodes (SPEs). We characterized the application of nickel-detecting biosensors with four different cultivated vegetables. We analyzed the efficiency of each nickel-detecting biosensor by potentiostat and atomic absorption spectrometry and compared the results from the sample analytes. We then analyzed the performance characteristics and responses of assembled biosensors, and show they are very effective at measuring nickel ions in food, especially with the urease-alginate biosensor affixed to silver SPEs, measured by cyclic voltammetry (sensitivity—2.1921 µA Mm−1 cm−2 and LOD—0.005 mg/L). Given the many advantages of biosensors, we describe an optimization pipeline approach to the application of different nickel-binding biosensors for public health, nutrition, and consumer safety, which are very promising.

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“…Hydrolysis of urease in urea generates ionic products, such as NH 4 + , HCO 3 À and OH À . The ions produced are monitored by several techniques, such as an optical transducer [5,6], potentiometry [7,8], colorimetry [9,10], amperometry [11,12], and conductometry [13,14].…”

Section: Introductionmentioning

confidence: 99%

Mathematical Model and Numerical Simulation of Conductometric Biosensor of Urea

et al. 2022

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In this article, a mathematical model was developed to describe and optimize the configuration of the urea biosensor. The biosensor is based on interdigitated gold microelectrodes modified with a urease enzyme membrane. The model presented here focuses on the enzymatic reaction and/or diffusion phenomena that occur in the enzyme membrane and in the diffusion layer. Numerical resolution of differential equations was performed using the finite difference technique. The mathematical model was validated using experimental biosensor data. The responses of the biosensor to various conditions were simulated to guide experiments, improve analytical performance, and reduce development costs.

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Paper-based analytical device with colorimetric detection for urease activity determination in soils and evaluation of potential inhibitors

Cited by 24 publications

References 64 publications

A Disposable Electrochemical Biosensor Based on Screen-Printed Carbon Electrodes Modified with Silver Nanowires/HPMC/Chitosan/Urease for the Detection of Mercury (II) in Water

A Disposable Electrochemical Biosensor Based on Screen-Printed Carbon Electrodes Modified with Silver Nanowires/HPMC/Chitosan/Urease for the Detection of Mercury (II) in Water

Development, Optimization, Characterization, and Application of Electrochemical Biosensors for Detecting Nickel Ions in Food

Mathematical Model and Numerical Simulation of Conductometric Biosensor of Urea

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