Paper-based 3D culture device integrated with electrochemical sensor for the on-line cell viability evaluation of amyloid-beta peptide induced damage in PC12 cells

Liu, Mengmeng; Liu, Hui; Li, Shanhong; Chen, Yao; Zhong, Yu; Lei, Yun; Lin, Xinhua; Liu, Ailin

doi:10.1016/j.bios.2019.111686

Cited by 17 publications

(14 citation statements)

References 23 publications

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“…Paper was also used as a medium for cell culture. In this case, the SPE platform allowed the on-line evaluation of cell viability by monitoring dopamine release from cell damage models [109]. Alternatively, the paper was also combined with a SPE card for sampling, as in the tear sampling system developed by Honikel et al using Whatman No 41 ashless filter paper [90].…”

Section: Spe Cards Combined With Paper Elementsmentioning

confidence: 99%

Paper-Based Screen-Printed Electrodes: A New Generation of Low-Cost Electroanalytical Platforms

Costa‐Rama

Fernández‐Abedul

2021

Biosensors

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Screen-printed technology has helped considerably to the development of portable electrochemical sensors since it provides miniaturized but robust and user-friendly electrodes. Moreover, this technology allows to obtain very versatile transducers, not only regarding their design, but also their ease of modification. Therefore, in the last decades, the use of screen-printed electrodes (SPEs) has exponentially increased, with ceramic as the main substrate. However, with the growing interest in the use of cheap and widely available materials as the basis of analytical devices, paper or other low-cost flat materials have become common substrates for SPEs. Thus, in this revision, a comprehensive overview on paper-based SPEs used for analytical proposes is provided. A great variety of designs is reported, together with several examples to illustrate the main applications.

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Section: Spe Cards Combined With Paper Elementsmentioning

confidence: 99%

Paper-Based Screen-Printed Electrodes: A New Generation of Low-Cost Electroanalytical Platforms

Costa‐Rama

Fernández‐Abedul

2021

Biosensors

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“…[25][26][27] The core peptide residues of Ab hydrophilic peptides are 17 to 21 and 30 to 42 which have been connected to the aggregation methods, so that 17 to 21 residues inhibit similar degree of the hydrophobic environments yet have a very less ratio to adopt the b-sheet confirmations. [28][29][30] From the recent reports, Shyam et al described the linear polyethylene glycol polymer-based micelles nanocomposition frame of BACE1 in the brain via infusing the nanocomposition in mouse lateral ventricle. 31 However, the nanocomposition meets various clinical strategies for the translation, for example, invasive administration protocols or risk of the immune or inflammatory actions.…”

Section: Introductionmentioning

confidence: 99%

“… 25 - 27 The core peptide residues of Aβ hydrophilic peptides are 17 to 21 and 30 to 42 which have been connected to the aggregation methods, so that 17 to 21 residues inhibit similar degree of the hydrophobic environments yet have a very less ratio to adopt the β-sheet confirmations. 28 - 30 …”

Section: Introductionmentioning

confidence: 99%

Lactoferrin-Loaded PEG/PLA Block Copolymer Targeted With Anti-Transferrin Receptor Antibodies for Alzheimer Disease

Xiang-hong

Wan

et al. 2020

Dose-Response

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Last few years, struggles have been reported to develop the nanovesicles for drug delivery via the brain–blood barrier (BBB). Novel drugs, for instance, iAβ5, are efficient to inhibit the aggregates connected to the treatment of Alzheimer disease and are being evaluated, but most of the reports reflect some drawbacks of the drugs to reach the brain in preferred concentrations owing to the less BBB penetrability of the surface dimensions. In this report, we designed and developed a new approach to enhance the transport of drug via BBB, constructed with lactoferrin (Lf)-coated polyethylene glycol-polylactide nanoparticles (Lf-PPN) with superficial monoclonal antibody-functionalized antitransferrin receptor and anti-Aβ to deliver the iAβ5 hooked on the brain. The porcine brain capillary endothelial cells were utilized as BBB typically to examine the framework efficacy and toxicity. The cellular uptake of the immuno-nanoparticles with measured conveyance of the iAβ5 peptide was significantly enhanced and associated with Lf-PPN without monoclonal antibody functionalizations.

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“…A particular challenge exists for pH measurements of airway epithelial cells grown under air-liquid interface culture conditions on Transwell inserts as there is a small growth surface area contributing to a low volume and depth of ASL. Since the measurement of pH in a cell culture system has been recognized as a useful tool to study different aspects of the cells' biology, various pH sensors have been developed [27][28][29][30][31][32] . Several techniques, such as optical sensing methods [33][34][35] , ion-sensitive field-effect transistor-based sensors, or electrochemical pH sensing materials 36 , have been investigated to monitor pH in a biological application.…”

mentioning

confidence: 99%

PHAIR: a biosensor for pH measurement in air–liquid interface cell culture

Dabaghi

Saraei

et al. 2021

Sci Rep

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In many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available pH sensors are difficult to integrate into a small-scale cell culture system due to their size and are not cost-effective for disposable use. We made PHAIR—a new pH sensor that uses a micro-wire format to measure pH in vitro human airway cell culture. Tungsten micro-wires were used as the working electrodes, and silver micro-wires with a silver/silver chloride coating were used as a pseudo reference electrode. pH sensitivity, in a wide and narrow range, and stability of these sensors were tested in common standard buffer solutions as well as in culture media of human airway epithelial cells grown at the air–liquid interface in a 24 well cell culture plate. When measuring the pH of cells grown under basal and challenge conditions using PHAIR, cell viability and cytokine responses were not affected. Our results confirm that micro-wire-based sensors have the capacity for miniaturization and detection of diverse ions while maintaining sensitivity. This suggests the broad application of PHAIR in various biological experimental settings.

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Paper-based 3D culture device integrated with electrochemical sensor for the on-line cell viability evaluation of amyloid-beta peptide induced damage in PC12 cells

Cited by 17 publications

References 23 publications

Paper-Based Screen-Printed Electrodes: A New Generation of Low-Cost Electroanalytical Platforms

Paper-Based Screen-Printed Electrodes: A New Generation of Low-Cost Electroanalytical Platforms

Lactoferrin-Loaded PEG/PLA Block Copolymer Targeted With Anti-Transferrin Receptor Antibodies for Alzheimer Disease

PHAIR: a biosensor for pH measurement in air–liquid interface cell culture

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Paper-based 3D culture device integrated with electrochemical sensor for the on-line cell viability evaluation of amyloid-beta peptide induced damage in PC12 cells

Cited by 17 publications

References 23 publications

Paper-Based Screen-Printed Electrodes: A New Generation of Low-Cost Electroanalytical Platforms

Paper-Based Screen-Printed Electrodes: A New Generation of Low-Cost Electroanalytical Platforms

Lactoferrin-Loaded PEG/PLA Block Copolymer Targeted With Anti-Transferrin Receptor Antibodies for Alzheimer Disease

PHAIR: a biosensor for pH measurement in air–liquid interface cell culture

Contact Info

Product

Resources

About

Paper-based 3D culture device integrated with electrochemical sensor for the on-line cell viability evaluation of amyloid-beta peptide induced damage in PC12 cells