Papaya Genetic Diversity Assessed With Microsatellite Markers in Germplasm From the Caribbean Region

Perez, John Albeiro Ocampo; D'Eeckenbrugge, Géo Coppens; Risterucci, Ange-Marie; Dambier, Dominique; Ollitrault, Patrick

doi:10.17660/actahortic.2007.740.9

Cited by 20 publications

(16 citation statements)

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“…Distance bootstrap values are given together with subgroups in parenthesis levels of multiallelism at all 59 SSR loci analyzed. Mean number of alleles per locus (4.02, for 59 loci) and expected heterozygosity (mean of 0.59) were higher than those reported by Ocampo Pérez et al (2006) who used 26 polymorphic markers, and observed 3.8 alleles per locus and H O and H E values of 0.42 and 0.57, respectively, but intermediate for samples collected in Guadeloupe, Venezuela, Colombia, Barbados and Costa Rica, that showed 6.6 alleles per locus for 15 loci, and H E values between 0.37 and 0.69 (Ocampo Pérez et al 2007). …”

Section: Discussioncontrasting

confidence: 64%

Polymorphism of Microsatellite Markers in Papaya (Carica papaya L.)

et al. 2010

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A set of 81 new microsatellite markers for Carica papaya L. previously identified by data mining using freely available sequence information from Genbank were tested for polymorphism using 30 germplasm accessions from the Papaya Germplasm Bank (PGM) at Embrapa Mandioca e Fruticultura Tropical (CNPMF) and 18 landraces. The data were used to estimate pairwise genetic distances between the genotypes. A neighbor-joining based dendrogram was used to define clusters and infer possible genetic structuring of the collection. Most microsatellites were polymorphic (73%), with an observed number of alleles per locus ranging from one to eleven. The levels of observed and expected heterozygosity for 51 polymorphic loci varied from 0.00 to 0.85 and from 0.08 to 0.82, averaging 0.19 and 0.59, respectively. Forty-four percent of microsatellites showed polymorphism information content (PIC) higher than 0.50. The compound microsatellites seem to be more informative than dinucleotide and trinucleotide repeats in average alleles per locus and PIC. Among dinucleotides, AG/TC or GA/CT repeat motifs exhibited more informativeness than TA/AT, GT/CA and TG/AC repeat motifs.The neighbor-joining analysis based on shared allele distance could differentiate all the papaya accessions and landraces as well as differences in their genetic structure. This set of markers will be useful for examining parentage, inbreeding and population structure in papaya.

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Section: Discussioncontrasting

confidence: 64%

Polymorphism of Microsatellite Markers in Papaya (Carica papaya L.)

et al. 2010

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“…The total number of alleles reported in diversity studies is usually proportional to the sample size, and some of the differences seen here may be attributed to sampling differences and plant material. Moreover, the observed heterozygosity values are remarkably low compared to those reported for SSRs in other genotypes, such as germplasm accessions (Ocampo Pérez et al 2007). The significant deficit in the frequency of heterozygotes observed was due to the origin of the material origin (inbred lines).…”

Section: Discussionmentioning

confidence: 60%

“…Microsatellites were first reported in C. papaya L. by Sharon et al (1992), Parasnis et al (1999), Santos et al (2003), Oliveira et al (2008), and Eustice et al (2008). Reliable and highly polymorphic SSR markers have been used mainly for genetic map construction (Chen et al 2007) and sexual differentiation (Parasnis et al 1999;Santos et al 2003) and to access genetic diversity (Ocampo Pérez et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Polymorphic microsatellite marker set for Carica papaya L. and its use in molecular-assisted selection

et al. 2010

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Conventional methods for the selection of papaya pure lines are time-consuming. Procedures involving the use of molecular markers and the indirect selection of homozygous plants can reduce this time considerably. The objective of this study was to evaluate the informativeness of a microsatellite marker set when used in marker-assisted selection (MAS) for the development of new papaya lines. Eighty-three lines originating from two segregating F 3 populations and from papaya germplasm were used for the molecular analysis of 27 microsatellite primers. Twenty polymorphic microsatellite primers were identified, allowing the identification of 86 alleles, with an average of 3.18 alleles per primer. The observed heterozygosity values were low for both the markers (0.00-0.29) and the individual lines (0.00-0.35). The inbreeding coefficient (f) ranged from 0.634 to 1.00. Eleven lines with f = 1.00 and 18 lines with f varying from 0.953 to 0.961 were identified. In addition, papaya lines showed high genetic diversity, which will certainly contribute to the development of new varieties. Our results show that the use of microsatellites in MAS is a quick and effective procedure for the development of papaya lines.

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“…Genetic diversity of cultivated and feral papayas has shown to be, in general, lower than wild populations: (1) Using AFLPs markers, a genetic similarity of 0.880 has been reported for 63 cultivated papaya accessions (Kim et al, 2002); (2) an expected heterozygosity (He) ranging from 0.54 to 0.69 in cultivars from the Caribbean region (Ocampo Pérez et al, 2005); (3) During domestication, gene flow between wild and domesticated conspecific plants plays an important role in addition to other evolutionary forces such as genetic drift and selection. In many cases, crop plants and their wild progenitors belong to the same biological species (Gepts, 2014;Rendón-Anaya et al, 2017), as in papaya, or cohabit with close wild relatives.…”

Section: Genetics and Genomicsmentioning

confidence: 99%

Domestication and Genetics of Papaya: A Review

Chávez‐Pesqueira

Núñez‐Farfán

2017

Front. Ecol. Evol.

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A wealth of plant species used by humans for different purposes, but mainly as food, originated and domesticated in the Mesoamerican region. Papaya (Carica papaya) is the third most cultivated tropical crop worldwide, and it has been hypothesized that Mesoamerica is the most likely center of its origin and domestication. In support of it, many wild populations of papaya occur throughout Mesoamerica and hence represent the gene pool of genetic variability for further evolution and future crop management. Despite its importance, a dearth of information exists regarding the status of wild populations of papaya, as compared to the extent of knowledge, and interest, on domesticated varieties. We review the evidence on the extant wild populations of papaya, as well as its origin and distribution. Also, we synthetize what is known on the domestication history of the species, including the domestication syndrome that distinguishes wild and domesticated papayas. Moreover, we make an account of the use of genetic markers to assess genetic diversity of wild and domesticated papaya, and discuss the importance of papaya as the first species with a transgenic cultivar to be released for human consumption, and one that has its complete genome sequenced. Evidence from different disciplines strongly suggest that papaya originated and was domesticated in Mesoamerica, and that wild populations in the region possess, still, high genetic diversity compared to the domesticated papaya. Finally, we outline papaya as an excellent model species for genomic studies that will help gain insight into the domestication process and improvement of papaya and other tropical crops.

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Papaya Genetic Diversity Assessed With Microsatellite Markers in Germplasm From the Caribbean Region

Cited by 20 publications

References 0 publications

Polymorphism of Microsatellite Markers in Papaya (Carica papaya L.)

Polymorphism of Microsatellite Markers in Papaya (Carica papaya L.)

Polymorphic microsatellite marker set for Carica papaya L. and its use in molecular-assisted selection

Domestication and Genetics of Papaya: A Review

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