Papain-stabilized silver nanoclusters: dual emission and ratiometric fluorescent sensing of ferrous ions

Abstract

Herein, a dual emissive fluorescent probe for Fe2+ based on papain-stabilized Ag nanoclusters was developed.

Poly(methacrylic acid)‐Stabilized Silver Nanoclusters as Colorimetric Sensors for the Rapid and Sensitive Detection of Ascorbic Acid

“…Many methodologies have been developed for preparing AgNCs, which rely on the use of different organic or biological templates, such as proteins, peptides, and polyelectrolytes [25,26] . Remarkably, the use of scaffolds helps to prevent particle aggregation during nanocluster synthesis.…”

“…[21][22][23][24] Many methodologies have been developed for preparing AgNCs, which rely on the use of different organic or biological templates, such as proteins, peptides, and polyelectrolytes. [25,26] Remarkably, the use of scaffolds helps to prevent particle aggregation during nanocluster synthesis. Notably, most of these studies have reported the use of AgNCs as fluorescent probes for the detection of a wide range of analytes, such as metal ions [27] and organic compounds [28,29] with approaches relying on measuring fluorescence quenching.…”

Recent Progress in Fluorescent Probes for the Detection of Ferrous Ion

“…另一种 是只由一种组分构成的探针(图 2), 该探针中的识别单 元和荧光发色基团是同一结构单元, 该类探针的合成过 程相对简单 [25][26] . 对于 Fe 2＋ 的检测, 一方面由于 Fe 2＋ 和 Fe 3＋ 不同的氧 化态彼此互变, 导致很难建立高特异性的探针; 另一方 面, 由于顺磁性的 Fe 2＋ 和荧光团之间强的作用力通常会 诱导荧光猝灭, 造成所构建的探针一部分是"on-off"型 的, 这类探针受生物自发荧光的影响, 不便于观察 [27][28][29] . 基于"off-on"型的 Fe 2＋ 响应探针数量也很少, 而且大多 数只被应用到细胞中 Fe 2＋ 的检测, 而构建能够特异性检 测动物模型中某一器官 Fe 2＋ 的浓度变化情况的探针, 还 有待于深入发掘研究 [30][31][32] .…”