Panton-Valentine leucocidin gene carriage among Staphylococcus aureus strains recovered from skin and soft tissue infections in Turkey

Demir, Tülin; Çöplü, Nilay; Bayrak, Hasan; Turan, Meral; Buyukguclu, Tuncay; Aksu, Neriman; Ekşioğlu, Meral; Yalçın, Başak; Atakan, NilgÃ1⁄4n; Kılıç, Selçuk; Karahan, Zeynep Ceren; Esen, Berrin

doi:10.1093/jac/dkr568

Cited by 15 publications

(16 citation statements)

References 7 publications

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“…The prevalence of MRSA was higher in our study compared to similar studies from Turkey (31.81%) (16), Beijing (3%) (17), Egypt (47.37%) (18), and Sao Paulo (10.6%) (15). It was similar to the rate reported from Torrance, California (66.57%) (3), and lower than the rate found in a general report from the United States (79%) (19).…”

Section: Discussionsupporting

confidence: 69%

Epidemiology and molecular characteristics of methicillin-resistantStaphylococcus aureus from skin and soft tissue infections in Shiraz, Iran

Mehrshad¹,

Haghkhah²,

Aghaei³

2017

Turk J Med Sci

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IntroductionAmong staphylococcal species, Staphylococcus aureus has been shown as a clinically relevant human pathogen, and in many individuals it may lead to asymptomatic colonization (1). Various infections from skin and soft tissue infections (SSTIs) to life-threatening infections like endovascular infections, pneumonia, septic arthritis, endocarditis, osteomyelitis, and sepsis are caused by this bacterium (2). Carriage of S. aureus is a risk factor for subsequent infection in various settings. Recent investigations suggest that nares-only screening may underestimate the prevalence of S. aureus colonization, and accurate determination requires sampling from other body sites (3). The spread of antibiotic resistance among strains of S. aureus has been dramatically elevated, such that methicillin-resistant S. aureus (MRSA) is recognized as a nosocomial pathogen worldwide. MRSA strains were once exclusively limited to hospital care; however, after outbreaks of infection in hospitals and health care facilities they have increasingly been detected among patients in the community who lack conventional risk factors for MRSA infection (4). MRSA represents a significant cause of morbidity and mortality in both hospital and community settings, which raises serious concerns in the treatment of staphylococcal infections; hence, the accurate and early determination of methicillin resistance is a necessity for the prognosis of S. aureus infections (5). Most MRSA and some methicillin-susceptible S. aureus (MSSA) isolates produce Panton-Valentine leukocidin (PVL), a pore-forming toxin consisting of two subunits S (LukE) and F (LukD) (6,7). It has become increasingly important as a virulence factor in necrotizing SSTIs such as furuncle and pneumonia (6). Both MSSA and community-associated MRSA can express PVL (8). Contrary to European countries (9), PVL-associated infections are increasing in some regions of Iran (10). This study aimed to assess the microbiological, epidemiological, and clinical features of purulent skin infections caused by S. aureus in Shiraz, southern Iran.Background/aim: Panton-Valentine leukocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) infections are increasing in some regions of Iran. The aim of the current study was to assess the epidemiology and molecular characteristics of S. aureus isolated from patients with skin infections in Shiraz, Iran.Materials and methods: Swab samples were obtained from patients admitted to the skin and burn units of hospitals. The medical records of each patient were collected via questionnaire. All staphylococcal isolates were collected and examined by conventional methods for detecting S. aureus strains. PCR was used to detect S. aureus harboring the mecA and pvl genes.Results: Out of 243 staphylococcal isolates, 55 (22.6%) S. aureus and 91 (37.4%) S. epidermidis were detected. Of the 45 patients, 21 (46.7%) were S. aureus carriers. The mecA gene was identified in 60% of S. aureus isolates, and the rest were sensitive to methicillin. Of the S. aur...

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Section: Discussionsupporting

confidence: 69%

Epidemiology and molecular characteristics of methicillin-resistantStaphylococcus aureus from skin and soft tissue infections in Shiraz, Iran

Mehrshad¹,

Haghkhah²,

Aghaei³

2017

Turk J Med Sci

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“…The incidence of CA‐MRSA in European countries is considered to be lower than that in the United States . In Turkey, Demir et al and Gulmez et al determined that Panton‐Valentine leukocidin‐carrying CA‐MRSA does not seem to be important yet .…”

Section: Introductionmentioning

confidence: 99%

Risk Factors for Intensive Care Unit Acquired Nasal Colonization of MRSA and Its Impact on MRSA Infection

Altınbaş

Shorbagi

Aşçıoğlu

et al. 2013

Clinical Laboratory Analysis

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“…The genotyping of Brazilian MRSA isolates allowed a better understanding of the dissemination patterns of the epidemic clones, such as, typically HA-MRSA and CA-MRSA 4 . The known virulence factor the exoprotein Panton-Valentine leucocidin (PVL) is traditionally seen in CA-MRSA, 5 however it has already been identified in methicillin-susceptible S. aureus and some rare HA-MRSA isolates 1, 6. The increasing number of reported infections caused by CA-MRSA strains in Brazil justifies the use of surveillance studies to help in understanding how these strains arrive and circulate in healthcare settings 2 .…”

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confidence: 99%

“…CA-MRSA isolates are recognized as presenting a higher spectrum of susceptible to non-β-lactam antibiotics when compared with HA-MRSA. However, vancomycin remains first-choice antibiotic therapy for serious infections 6 . The increase use of this late antimicrobial drug is directly related to the emergence of vancomycin-intermediate (VISA), vancomycin-resistant (VRSA) and varied multidrug-resistant MRSA isolates around the world 15, 16.…”

mentioning

confidence: 99%

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from blood in Rio de Janeiro displaying susceptibility profiles to non-β-lactam antibiotics

Zuma

Lima

Carvalho-Assef

et al. 2017

Brazilian Journal of Microbiology

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The distinction between healthcare-associated MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA) infections has become increasingly blurred. We assessed the molecular characterization and antimicrobial resistance profile for MRSA isolates from blood. Most of all (81.9%) isolates are related to known HA-MRSA and CA-MRSA epidemic lineages, such as, USA300, USA400, USA600, USA800 and USA1100. This is the first multicenter study in Rio de Janeiro.

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Panton-Valentine leucocidin gene carriage among Staphylococcus aureus strains recovered from skin and soft tissue infections in Turkey

Abstract: Based on the findings of this study, infection related to PVL-carrying CA-MRSA is not at an alarmingly high level, but population-based surveillance studies should be done to determine the real status.

Cited by 15 publications

References 7 publications

Epidemiology and molecular characteristics of methicillin-resistantStaphylococcus aureus from skin and soft tissue infections in Shiraz, Iran

Epidemiology and molecular characteristics of methicillin-resistantStaphylococcus aureus from skin and soft tissue infections in Shiraz, Iran

Risk Factors for Intensive Care Unit Acquired Nasal Colonization of MRSA and Its Impact on MRSA Infection

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from blood in Rio de Janeiro displaying susceptibility profiles to non-β-lactam antibiotics

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