Pannexin-1 channels do not regulate α1-adrenoceptor-mediated vasoconstriction in resistance arteries

Angus, James A.; Betrie, Ashenafi H; Wright, Christine E.

doi:10.1016/j.ejphar.2015.01.024

Cited by 12 publications

(9 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present 1 study showed mefloquine to inhibit responses to both 5-HT and U46619 (a thromboxane mimetic), indicating that mefloquine is not a selective pannexin inhibitor at the concentrations used in this study. Such argument was supported by a recent study by Angus et al (2015), who recently showed mefloquine (10μM to 20 μM) to demonstrate a wide range of inhibitory actions (Angus et al, 2015). For example, mefloquine (3μM to 10μM) inhibited the contractions mediated by vasopressin, phenylephrine, endothelin-1, U46619, sympathetic nerve stimulation and K (40 mM) in rat + and mouse small mesenteric arteries.…”

Section: Discussionmentioning

confidence: 90%

“…For example, Sridharan et al (2010) showed carbenoxolone (100µM) to inhibit the hypnotically induced ATP release from human erythrocytes (Sridharan et al, 2010). Similarly, Angus et al (2015) showed that carbenoxolone had no effect on agonist or nerve-induced activation of alpha-adrenoceptors in rat small mesenteric resistance arteries (Angus et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

An examination into the function of pannexin in noradrenaline induced vasoconstriction in porcine splenic artery

Habib¹

2020

Asian J Pharm Pharmacol

View full text Add to dashboard Cite

Background: Pannexins are newly discovered proteins that were first discovered by Panchin et al. in 2000. This aim of this work was to examine the presence and function of pannexins in the porcine splenic artery (PSA) in which α 1A-adrenoceptors is present. The involvement of pannexin channels was studied using Materials and Methods: several pannexin inhibitors, i.e. mefloquine (a non-selective pannexin inhibitor), probenecid (a selective pannexin-1 inhibitor at low concentrations) and carbenoxolone (a selective pannexin-1 inhibitor). Additionally, the involvement of ATP (via activation of P2 purinoceptors) in NA-induced contractile responses as well as sympathetic nerve activation was examined using P2 purinoceptor antagonists (PPADS and suramin). Our data showed that both pannexin-Results: 1 and pannexin-2 are present in the PSA. Mefloquine and probenecid reduced the responses to both noradrenalineinduced contractions and the frequency-dependent response curves generated to sympathetic nerve stimulation, whereas carbenoxolone, suramin and PPADS had no effect on responses to neither exogenous NA nor those caused by activating the sympathetic nerves, arguing against the role of ATP in mediating noradrenaline-induced responses in the PSA. This is because mefloquine demonstrated non-selective inhibitory actions on contractile responses since it was also shown to inhibit responses to 5-HT and U46619 (the thromboxane mimetic). The present work Conclusion: therefore suggests evidence for the involvement of pannexin channels in conducting responses to NA-induced-α 1 adrenoceptor-mediated vasoconstriction in blood vessels in PSA, although great care must be taken in interpreting this data on the basis of a lack of selectivity of the pharmacological agents currently available as pannexin inhibitors.

show abstract

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

An examination into the function of pannexin in noradrenaline induced vasoconstriction in porcine splenic artery

Habib¹

2020

Asian J Pharm Pharmacol

View full text Add to dashboard Cite

show abstract

“…The present study showed mefloquine to inhibit responses to both 5-HT and U46619 (a thromboxane mimetic), indicating that mefloquine is not a selective pannexin inhibitor at the concentrations used in this study. Such argument was supported by a recent study by Angus et al (2015), who recently showed mefloquine (10 μM to 20 μM) to demonstrate a wide range of inhibitory actions [27]. For example, mefloquine (3 μM to 10 μM) inhibited the contractions mediated by vasopressin, phenylephrine, endothelin-1, U46619, sympathetic nerve stimulation and K + (40 mM) in rat and mouse small mesenteric arteries.…”

Section: Effect Of Pannexin Inhibition On Responses To Na and Efs In mentioning

confidence: 90%

“…For example, Sridharan et al (2010) showed carbenoxolone (100 μM) to inhibit the hypnotically induced ATP release from human erythrocytes [26]. Similarly, Angus et al (2015) showed that carbenoxolone had no effect on agonist or nerve-induced activation of alpha-adrenoceptors in rat small mesenteric resistance arteries [27].…”

Section: Effect Of Pannexin Inhibition On Responses To Na and Efs In mentioning

confidence: 99%

An examination into the function of pannexin in noradrenaline induced vasoconstriction in porcine splenic artery

Habib

2020

Preprint

View full text Add to dashboard Cite

Background: Pannexins are newly discovered proteins that were first discovered by Panchin et al. in 2000. This aim of this work was to examine the presence and function of pannexins in the porcine splenic artery (PSA) in which α1A-adrenoceptors are present. Materials and Methods: The involvement of pannexin channels was studied using several pannexin inhibitors, i.e. mefloquine (a non-selective pannexin inhibitor), probenecid (a selective pannexin-1 inhibitor at low concentrations) and carbenoxolone (a selective pannexin-1 inhibitor). Additionally, the involvement of ATP (via activation of P2 purinoceptors) in NA-induced contractile responses as well as sympathetic nerve activation was examined using P2 purinoceptor antagonists (PPADS and suramin). Results: Our data showed that both pannexin-1 and pannexin-2 are present in the PSA. Mefloquine and probenecid reduced the responses to both noradrenaline-induced contractions and the frequency-dependent response curves generated to sympathetic nerve stimulation, whereas carbenoxolone, suramin and PPADS had no effect on responses to neither exogenous NA nor those caused by activating the sympathetic nerves, arguing against the role of ATP in mediating noradrenaline-induced responses in the PSA. This is because mefloquine demonstrated non-selective inhibitory actions on contractile responses since it was also shown to inhibit responses to 5-HT and U46619 (the thromboxane mimetic). Conclusion: The present work therefore provided evidence for the involvement of pannexin channels in conducting responses to NA-induced α1-adrenoceptor-mediated vasoconstriction in blood vessels in PSA, although great care must be taken in interpreting this data on the basis of a lack of selectivity of the pharmacological agents currently available as pannexin inhibitors

show abstract

“…First, a study using another muscular resistance artery of Panx1-deficient mice reported enhanced rather than reduced phenylephrine-induced contractions [118]. Moreover, there is also no evidence that Panx1 channels releasing ATP have any role in the constrictor actions of alpha1-adrenoceptor activation of small resistance arteries [151]. Clearly, more studies are needed before a consensus will be reached regarding involvement of Panx1 in alpha1-adrenergic receptor-mediated effects.…”

Section: Pannexins In Resistance Arteries and Arteriolesmentioning

confidence: 99%

Role of connexins and pannexins in cardiovascular physiology

Meens

Kwak

2015

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Connexins and pannexins form connexons, pannexons and membrane channels, which are critically involved in many aspects of cardiovascular physiology. For that reason, a vast number of studies have addressed the role of connexins and pannexins in the arterial and venous systems as well as in the heart. Moreover, a role for connexins in lymphatics has recently also been suggested. This review provides an overview of the current knowledge regarding the involvement of connexins and pannexins in cardiovascular physiology.

show abstract

Pannexin-1 channels do not regulate α1-adrenoceptor-mediated vasoconstriction in resistance arteries

Cited by 12 publications

References 26 publications

An examination into the function of pannexin in noradrenaline induced vasoconstriction in porcine splenic artery

An examination into the function of pannexin in noradrenaline induced vasoconstriction in porcine splenic artery

An examination into the function of pannexin in noradrenaline induced vasoconstriction in porcine splenic artery

Role of connexins and pannexins in cardiovascular physiology

Contact Info

Product

Resources

About