Pancreatic lipase and α-amylase inhibitory activity of extracts from selected plant materials after gastrointestinal digestion in vitro

Siegień, Justyna; Buchholz, Tina; Popowski, Dominik; Granica, Sebastian; Osińska, Ewa; Melzig, Matthias F.; Czerwińska, Monika E.

doi:10.1016/j.foodchem.2021.129414

Cited by 30 publications

(17 citation statements)

References 66 publications

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“…Recent studies have demonstrated that pancreatic lipase is a key enzyme in the digestion and absorption of fat in the intestinal tract, which can hydrolyze 50%–70% dietary fat and convert it into glycerol monoesters and fatty acids in human body ( Siegien et al, 2021 , Yuan et al, 2018 ). The effect of inhibitor on pancreatic lipase activity intercepts the production of glycerol monoesters and fatty acids thereby enhancing the hypolipidemic activity.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the exploration of natural and no side effects of lipid-lowering drugs has become the focus of current research. Studies have found that polysaccharide, flavonoid, polyphenol and other active substances from algae and plants have significant inhibitory effects on pancreatic lipase and cholesterol esterase, showing an excellent effect of lowering blood lipid ( Huang et al, 2020 , Li et al, 2021 , Siegien et al, 2021 ). It has been proved that Ulva prolifera polysaccharide weakened the activity of pancreatic lipase, presenting the anti-hyperlipidemia activity ( Yuan et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Structural characterization and hypolipidemic activity of Gracilaria lemaneiformis polysaccharide and its degradation products

Long

Xiang

et al. 2022

Food Chemistry: X

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structural characterization and hypolipidemic activity of Gracilaria lemaneiformis polysaccharide and its degradation products

Long

Xiang

et al. 2022

Food Chemistry: X

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“…α‐Amylase is a key enzyme in the digestion of starch in the human body. The rate of carbohydrate conversion to glucose can be reduced by inhibiting the activity of α‐amylase (Cheng et al., 2017; Siegień et al., 2021). The α‐amylase inhibitory activity of WBIDFs is shown in Figure 6d.…”

Section: Resultsmentioning

confidence: 99%

Effects of acid treatment on the physicochemical and functional properties of wheat bran insoluble dietary fiber

et al. 2021

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Background and Objectives: Wheat bran is a by-product of the wheat milling industry but most are used as animal fodder or discarded. To extend the applications of wheat bran, the structural, physicochemical, and functional properties of sulfuric acid-modified wheat bran insoluble fibers (WBIDF) produced using 1%, 2%, 4%, and 8% H 2 SO 4 and 1.5% potassium hydroxide were investigated.Findings: Due to cell wall disintegration, 1.0% and 2.0% H 2 SO 4 treatments increased the concentration of soluble dietary fiber. Scanning electron microscopy indicated that acid treatment disrupted WBIDF structure, removed starch granules from the surface, and significantly reduced starch content. Higher H 2 SO 4 concentrations increased WBIDF crystallinity due to hydrolysis of hemicellulose and amorphous cellulose. Acid treatment below 2% not only enhanced the water holding, water swelling, oil binding, and cation exchange capacities of WBIDF, but also increased cholesterol, sodium cholate, and glucose adsorption capacities and α-amylase inhibition activity. Conclusions:Acid-modified WBIDF has potential as a functional food ingredient.Significance and novelty: Acid treatment can be used to disrupt the microstructure of wheat bran to improve its functional properties. These findings can be utilized to improve the technological functionality of wheat bran as a high fiber ingredient for food applications.

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“…To mimic in vitro digestion conditions, four categories were created—salivary, gastric, intestinal, and colon compartments, as it was previously described [ 22 ]. The assay was carried out in the presence (+E) or absence (−E) of standard gastrointestinal enzymes ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…The UV chromatograms were registered at 240, 280, 325 nm, or 520 nm. The conditions of ESI parameters were described previously [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Potentially Bio-Accessible Metabolites from an Extract of Cornus mas Fruit after Gastrointestinal Digestion In Vitro and Gut Microbiota Ex Vivo Treatment

et al. 2022

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Targeting pancreatic lipase and α-amylase by digestion-derived fractions of ethanolic-aqueous (60%, v/v) extract from Cornus mas fruit (CM) in relation to the control and prevention of metabolic disorders, including diabetes, was the first purpose of the present study. Taking into consideration the significance of bio-accessibility of compounds, we attempted to identify metabolites of CM after gastrointestinal digestion in vitro, as well as their kinetic changes upon gut microbiota treatment. The digestion of extract was simulated with digestive enzymes in vitro and human gut microbiota ex vivo (1 h, 3 h, 6 h, 24 h), followed by chromatographic analysis using the UHPLC-DAD-MSn method. The effect of fractions from gastrointestinal digestion in vitro on the activity of pancreatic lipase and α-amylase was studied with fluorescence-based assays. The gastric and intestinal fractions obtained after in vitro digestion of CM inhibited pancreatic lipase and α-amylase. Loganic acid as the main constituent of the extract was digested in the experimental conditions in contrast to cornuside. It was found in most analytes such as salivary, gastric, intestinal, and even colon (fecal slurry, FS) fractions. In all fractions, kaempferol hexoside and reduced forms of kaempferol, such as aromadendrin, and benzoic acid were assigned. The signals of tannins were detected in all fractions. Cornusiin A was tentatively assigned in the gastric fraction. The metabolites originating from kinetic analytes have been classified mainly as phenolic acids, hydrolyzable tannins, and flavonoids. Phenolic acids (protocatechuic acid, gallic acid), tannins (digalloylglucose, tri-O-galloyl-β-D-glucose), and flavonoids (aromadendrin, dihydroquercetin) were detected in the late phases of digestion in fecal slurry suspension. Cornuside was found in FS analyte after 3 h incubation. It was not detected in the samples after 6 and 24 h incubation with FS. In conclusion, cornuside, aromadendrin, and phenolic acids may be potentially bio-accessible compounds of CM. The presence of plants’ secondary metabolites in the intestinal fractions allows us to indicate them as responsible for decreasing glucose and lipid absorption.

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Pancreatic lipase and α-amylase inhibitory activity of extracts from selected plant materials after gastrointestinal digestion in vitro

Cited by 30 publications

References 66 publications

Structural characterization and hypolipidemic activity of Gracilaria lemaneiformis polysaccharide and its degradation products

Structural characterization and hypolipidemic activity of Gracilaria lemaneiformis polysaccharide and its degradation products

Effects of acid treatment on the physicochemical and functional properties of wheat bran insoluble dietary fiber

Potentially Bio-Accessible Metabolites from an Extract of Cornus mas Fruit after Gastrointestinal Digestion In Vitro and Gut Microbiota Ex Vivo Treatment

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