Pancreatic Islet Derived Stem Cells Can Express Co-stimulatory Molecules of Antigen-Presenting Cells

Karaöz, Erdal; Okçu, Alparslan; Sağlam, Özlem; Genc, Z.; Ayhan, Selda; Kasap, Murat

doi:10.1016/j.transproceed.2010.07.093

Cited by 7 publications

(7 citation statements)

References 37 publications

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“…Although initially found in the bone marrow, adult stem cells capable of self-renewal and differentiation into various mesodermal cell lineages have been identified in many other organs and tissues including adipose tissue, umbilical cord, blood, skin, teeth, testes, gut, liver and ovarian epithelium345. Unlike other stem cells such as Keratinocyte Stem cells678, or pancreatic islet-derived stem cells9, BMSCs derived from the bone marrow produce low levels or none of class I and II Major Histocompatibility Complex (MHC) antigens and lack CD40, CD80 and CD86, co-stimulatory molecules required for activation of T cells. Furthermore, BMSCs are able to migrate to the site of inflammation and suppress the function of lymphocytes (T and B)1011, natural killer cells12, dendritic cells13 and neutrophils14.…”

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confidence: 99%

Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study

Cizkova

Devaux

Marrec-Croq

et al. 2014

Sci Rep

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In the present paper we develop a new non-cell based (cell-free) therapeutic approach applied to BV2 microglial cells and spinal cord derived primary microglia (PM) using conditioned media from rat bone marrow stromal cells (BMSCs-CM). First we collected conditioned media (CM) from either naive or injured rat spinal cord tissue (SCI-CM, inflammatory stimulation agent) and from rat bone marrow stromal cells (BMSCs-CM, therapeutic immunomodulation agent). They were both subsequently checked for the presence of chemokines and growth, neurotrophic and neural migration factors using proteomics analysis. The data clearly showed that rat BMSCs-CM contain in vitro growth factors, neural migration factors, osteogenic factors, differentiating factors and immunomodulators, whereas SCI-CM contain chemokines, chemoattractant factors and neurotrophic factors. Afterwards we determined whether the BMSCs-CM affect chemotactic activity, NO production, morphological and pro-apoptotic changes of either BV2 or PM cells once activated with SCI-CM. Our results confirm the anti-migratory and NO-inhibitory effects of BMSCs-CM on SCI-CM-activated microglia with higher impact on primary microglia. The cytotoxic effect of BMSCs-CM occurred only on SCI-CM-stimulated BV2 cells and PM, not on naive BV2 cells, nor on PM. Taken together, the molecular cocktail found in BMSCs-CM is favorable for immunomodulatory properties.

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mentioning

confidence: 99%

Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study

Cizkova

Devaux

Marrec-Croq

et al. 2014

Sci Rep

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“…We showed the expression of all three genes in rPI-SCs (Karaoz et al 2010c ) (Table 10.2 ), implying a role played by rPI-SCs in the regulation of immunity and tissue homeostasis in islets and highlighting the antiapoptotic infl uence mechanisms that might have a protective role on pancreatic islet cells.…”

Section: Type 1 Diabetes and Stem Cells: A New Approachmentioning

confidence: 93%

“…Lei et al ( 2005 ) observed a similar case, in which murine-derived keratinocyte stem cells (KSCs) expressed CD80 but not CD86, indicating that KSCs could act as APCs (Lei et al 2005 ) . The absence of major histocompatibility complex (MHC) class II expression in rPI-SCs, whose constitutive expression was observed only in professional APCs of the immune system (Karaoz et al 2010c ) , supports that these cells are nonprofessional APCs.…”

Section: Presence Of Transcripts Coding For Antigen-presenting Surfacmentioning

confidence: 94%

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Mesenchymal Stem Cells: Possibilities of New Treatment Options

Tokcaer-Keskin

Koçak

Gürsel

et al. 2012

Adult and Embryonic Stem Cells

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“…The anti-inflammatory and antiapoptotic effect of PISC is probably due to the induction of negative T-regulatory cells and a subset of antiapoptotic molecular markers relevant to the putative cell replacement therapy for T1D ( Karaoz et al. , 2010a , b , c ).…”

Section: Identification Of New Therapeutic Strategies and Drug Targetmentioning

confidence: 99%

The double trouble of metabolic diseases: the diabetes–cancer link

Tudzarova

Osman

2015

MBoC

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The recent recognition of the clinical association between type 2 diabetes (T2D) and several types of human cancer has been further highlighted by reports of antidiabetic drugs treating or promoting cancer. At the cellular level, a plethora of molecules operating within distinct signaling pathways suggests cross-talk between the multiple pathways at the interface of the diabetes–cancer link. Additionally, a growing body of emerging evidence implicates homeostatic pathways that may become imbalanced during the pathogenesis of T2D or cancer or that become chronically deregulated by prolonged drug administration, leading to the development of cancer in diabetes and vice versa. This notion underscores the importance of combining clinical and basic mechanistic studies not only to unravel mechanisms of disease development but also to understand mechanisms of drug action. In turn, this may help the development of personalized strategies in which drug doses and administration durations are tailored to individual cases at different stages of the disease progression to achieve more efficacious treatments that undermine the diabetes–cancer association.

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Pancreatic Islet Derived Stem Cells Can Express Co-stimulatory Molecules of Antigen-Presenting Cells

Cited by 7 publications

References 37 publications

Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study

Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study

Mesenchymal Stem Cells: Possibilities of New Treatment Options

The double trouble of metabolic diseases: the diabetes–cancer link

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