Pancreatic islet autotransplantation: Results in dogs with chronic duct ligation

Preparation of Viable islet Cells from Dogs by a New Method

“…Recently, chronic pancreatitis has been treated by pancreatectomy and autotransplantation of pancreatic fragments in dogs 15 and humans. 16 - 17 However, results have not been satisfactory.…”

Effect of islet cell autograft from the highly scarred pancreas on pancreatectomized dogs.

“…However, it has been estimated that the B-cell procurement by a similar method is only 6 to 18% of the whole pancreas (Kretschmer et al 1978). Poor procurement of islet tissue possibly responsible for the poor control of diabetes mellitus of the animals through autotransplantation from a scarred pancreas after total pancreatecotomy (Mehigan et al 1980). On the other hand, Horaguchi and Merrell (1981) reported satisfactory procurement of 57% of islet tissue recovery with smaller contamination of the exocrine tissue by means of a new method.…”

“…However, autotransplantation in the experimental animals with chronic pancreatitis (Mehigan et al 1980) have generally brought about little beneficial effect mainly because of an inadequate procurement of islet tissue from the scarred pancreas, although presently the experimental autograft from the normal pancreas can control the diabetic state following total pancreatectomy (Mirkovitch and Campiche 1976;Kretschmer et al 1978;Mehigan et al 1980). Similarly in patients with chronic pancreatitis, it is difficult to make islet tissue preparations from the diseased pancreas, and islet autotranstplantation played only a limited role in controlling diabetes after total or near total pancreatectomy (Naj arian et al 1980; Traverso et al 1981).…”

Pancreas and islet transplantation

“…Mehigan et al [107] found that prior duct ligation gave a less satisfactory preparation. Elimination of the steps required for purification shortened the islet preparation time, increased the recovery, and reduced the need for multiple donors.…”

“…Mehigan et al [107] found that the success rate was lower with transplantation of tissue prepared from dogs with chronic pancreatitis induced by duct ligation even though the insulin reserve of the pancreas before dispersion was nearly normal.…”