Pan-p63 but not ΔNp63 (p40) expression in undifferentiated carcinoma of the pancreas

Abstract

Undifferentiated carcinoma of the pancreas (UC) is a carcinoma without a definitive direction of differentiation. Tumour protein p63 is a regulator of squamous phenotype, which may also be engaged in tumour development. N-terminal isoforms of p63 are TAp63 and ΔNp63. Pan-p63 antibodies are able to detect both isoforms, whereas p40 antibodies recognise the ΔNp63 isoform only. The aim of the study was to describe pan-p63/p40 immunohistochemical expression patterns in pancreatic neoplasms: UC, ductal adenocarcinomas, neuroendocrine tumours, neuroendocrine carcinomas, serous cystic neoplasms, and solid pseudopapillary neoplasms. DAK-p63 and BC28 antibodies were used for pan-p63 and p40 detection, respectively. Moderate-to-strong pan-p63 was found in anaplastic (pleomorphic giant cell) UC (n = 4), sarcomatoid UC (n = 2), UC with osteoclast-like giant cells (n = 3), and ductal carcinomas with partial squamous differentiation. Weak and focal pan-p63 expression was found in monomorphic UC (n = 3) and in the majority of neuroendocrine carcinomas (6/7 cases). Pan-p63 expression was infrequent in ductal carcinomas without squamous differentiation and in neuroendocrine tumours. Serous cystic and solid pseudopapillary neoplasms were pan-p63-negative. Ductal carcinomas with partial squamous differentiation were the only tumours with evident p40 expression. Pan-p63(+)/p40(-) immunohistochemical status may be supportive for UC diagnosis. The pan-p63 expression was not equivalent to squamous differentiation in pancreatic neoplasia.

The Pyroptosis Related Genes Signature Predicts Prognosis and Immune Infiltration of Tumor Microenvironment in Hepatocellular Carcinoma

“…Tianze Zhang found that transcription factor p53 suppresses tumor growth by prompting pyroptosis in nonsmall-cell lung cancer [40]. N-terminal isoforms of p63 are TAp63 and ΔNp63 [41]. The findings of the present study suggested that lncRNA RP1-85F18.6 may trigger colorectal cancer cell proliferation, invasion and cell cycle disruption, and suppress apoptosis and pyroptosis of colorectal cancer cells through regulating ΔNp63 expression [42].…”

The High Presence of HCMV pp71 Proteins, Correlate with P63 Expression in Pancreatic Cancer Tumor Tissue

“…Another research on undifferentiated pancreatic cancer (UC) discovered moderate-to-strong pan-p63 expression in anaplastic (pleomorphic giant cell) UC (n = 4), sarcomatoid UC (n = 2), UC with osteoclastlike giant cells (n = 3), and ductal carcinomas with partial squamous differentiation (n = 3). Pan-p63 expression was observed to be weak and localized in monomorphic UC (n = 3) and in the majority of neuroendocrine carcinomas (6/7 cases) (Liszka, 2020).…”

Pyroptosis related genes signature predicts prognosis and immune infiltration of tumor microenvironment in hepatocellular carcinoma

“…Zhang et al found that transcription factor p53 suppressed tumor growth by prompting pyroptosis in non-small-cell lung cancer [ 41 ]. N-terminal isoforms of p63 are TAp63 and ΔNp63 [ 42 ]. These findings suggested that lncRNA RP1-85F18.6 may trigger colorectal cancer cell proliferation, invasion and cell cycle disruption, and suppressed the apoptosis and pyroptosis of colorectal cancer cells through regulating ΔNp63 expression [ 43 ].…”