Pan-myeloid Differentiation of Human Cord Blood Derived CD34&lt;sup&gt;+&lt;/sup&gt; Hematopoietic Stem and Progenitor Cells

Bapat, Aditi; Keita, Nakia; Sharma, Shalini

doi:10.3791/59836

Cited by 3 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…32,33 Cluster 10 was annotated as hematopoietic stem cell (HSC) with high expression of CD34, NT5E, VCAM1, MCAM, and SELE. [34][35][36] As a further validation, the expected gene ontology (GO) terms were also found in gene-specific expression cell clusters (Figure 1E).…”

Section: Scrna-seq Identified Multiple Cell Types In Control Subjects and Ais Patientsmentioning

confidence: 68%

See 1 more Smart Citation

Single‐cell RNA Seq reveals cellular landscape‐specific characteristics and potential etiologies for adolescent idiopathic scoliosis

Yang

Shi

et al. 2021

JOR Spine

View full text Add to dashboard Cite

Backgrounds: Abnormal vertebral growth and development have been found in adolescent idiopathic scoliosis (AIS) patients, and the proliferation and differentiation of bone development-related cells play important roles in its pathogenesis. However, a comprehensive single-cell-level differentiation roadmap in AIS has not been achieved. Methods:The present study compared the single-cell level cellular landscapes of spinal cancellous bone tissues between AIS patients and healthy subjects using high throughput single-cell RNA sequencing (scRNA-seq), which covers multiple cellular lineages including osteoblast, chondrocyte, osteoclast and related immunocytes. We constructed the differentiation trajectories of bone development-related cell lineages through pseudotime analysis, and the intercellular-communication networks between bone development-related cells and immunocytes were further developed.Results: A total of 11 distinct cell clusters were identified according to the genome-wide transcriptome profiles. t-Distributed stochastic neighbor embedding (t-SNE) analysis showed that mesenchymal stem cells (MSC) were classified into three subtypes: MSC-LOXL2, MSC-IGFBP5, and MSC-GJA1. Gene ontology (GO) analysis showed that MSC-GJA1 might possess greater osteoblast differentiation potential than the others. MSC-IGFBP5 was the specific MSC subtype observed only in AIS. There were two distinct gene expression clusters: OB-DPT and OB-OLFML2B, and the counts of osteoblasts derived from AIS was significantly less than that of non-AIS subjects. In AIS patients, MSC-IGFBP5 failed to differentiate into osteoblasts and exhibited negative regulation of cell proliferation and enhanced cell death. CPC-PCNA was found to be the specific chondrocyte progenitor cell (CPC) subtype observed only in AIS patients. The cell counts of OC-BIRC3 in AIS were less than those in controls. Pseudotime analysis suggested two possible distinct osteoclast differentiation patterns in AIS and control subjects. Monocytes in AIS mainly differentiated into OC-CRISP3.

show abstract

Section: Scrna-seq Identified Multiple Cell Types In Control Subjects and Ais Patientsmentioning

confidence: 68%

“… 32 , 33 Cluster 10 was annotated as hematopoietic stem cell (HSC) with high expression of CD34, NT5E, VCAM1, MCAM, and SELE. 34 , 35 , 36 …”

Section: Resultsmentioning

confidence: 99%

Single‐cell RNA Seq reveals cellular landscape‐specific characteristics and potential etiologies for adolescent idiopathic scoliosis

Yang

Shi

et al. 2021

JOR Spine

View full text Add to dashboard Cite

show abstract

“…IL-3 and GM-CSF: IL-3 and GM-CSF promote the survival, differentiation and proliferation of a range of myeloid progenitor cells [ 236 , 240 , 241 ]. Indeed, the expression of IL-3 receptor (IL-3R, or CD123) is itself a marker for the common myeloid progenitor and granulocytic/monocytic progenitor cell identity [ 242 ].…”

Section: In Vitro Differentiation Into Myeloid Cellsmentioning

confidence: 99%

In Vitro Human Haematopoietic Stem Cell Expansion and Differentiation

Bozhilov

Hsu

Brown

et al. 2023

Cells

View full text Add to dashboard Cite

The haematopoietic system plays an essential role in our health and survival. It is comprised of a range of mature blood and immune cell types, including oxygen-carrying erythrocytes, platelet-producing megakaryocytes and infection-fighting myeloid and lymphoid cells. Self-renewing multipotent haematopoietic stem cells (HSCs) and a range of intermediate haematopoietic progenitor cell types differentiate into these mature cell types to continuously support haematopoietic system homeostasis throughout life. This process of haematopoiesis is tightly regulated in vivo and primarily takes place in the bone marrow. Over the years, a range of in vitro culture systems have been developed, either to expand haematopoietic stem and progenitor cells or to differentiate them into the various haematopoietic lineages, based on the use of recombinant cytokines, co-culture systems and/or small molecules. These approaches provide important tractable models to study human haematopoiesis in vitro. Additionally, haematopoietic cell culture systems are being developed and clinical tested as a source of cell products for transplantation and transfusion medicine. This review discusses the in vitro culture protocols for human HSC expansion and differentiation, and summarises the key factors involved in these biological processes.

show abstract

The effect of Acellularized Wharton's Jelly-derived exosomes on myeloid differentiation of umbilical cord blood-derived CD34+ hematopoietic stem cells

et al. 2021

View full text Add to dashboard Cite

Pan-myeloid Differentiation of Human Cord Blood Derived CD34<sup>+</sup> Hematopoietic Stem and Progenitor Cells

Cited by 3 publications

References 28 publications

Single‐cell RNA Seq reveals cellular landscape‐specific characteristics and potential etiologies for adolescent idiopathic scoliosis

Single‐cell RNA Seq reveals cellular landscape‐specific characteristics and potential etiologies for adolescent idiopathic scoliosis

In Vitro Human Haematopoietic Stem Cell Expansion and Differentiation

The effect of Acellularized Wharton's Jelly-derived exosomes on myeloid differentiation of umbilical cord blood-derived CD34+ hematopoietic stem cells

Contact Info

Product

Resources

About