Pan-myeloid Differentiation of Human Cord Blood Derived CD34&lt;sup&gt;+&lt;/sup&gt; Hematopoietic Stem and Progenitor Cells

Bapat, Aditi; Keita, Nakia; Sk, Sharma

doi:10.3791/59836-v

Cited by 2 publications

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“…Different in vitro and ex vivo model systems using miscellaneous culture conditions have been developed over the past few decades to propagate proliferation of HSPCs and development of specific hematopoietic lineages. 1 , 2 , 3 However, relatively few studies exist that have investigated development of both human lymphoid and myeloid lineages concurrently under the same conditions. In this study, we report the characterization and use of an in vitro stromal cell–free model system that allows temporal study of early human HSPC differentiation into multiple lineage–specified immune progenitor cells.…”

Section: Introductionmentioning

confidence: 99%

An in vitro model of human hematopoiesis identifies a regulatory role for the aryl hydrocarbon receptor

et al. 2023

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In vitro models to study simultaneous development of different human immune cells and hematopoietic lineages are lacking. We identified and characterized, using single-cell methods, an in vitro stromal-cell free culture system of human hematopoietic stem and progenitor cell (HSPC) differentiation that allows concurrent development of multiple immune cell lineages. The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor influencing many biological processes in diverse cell types. Using this in vitro model, we found that AHR activation by the highly specific AHR ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), drives differentiation of human umbilical cord-blood derived CD34+ HSPCs towards monocytes and granulocytes with a significant decrease in lymphoid and megakaryocyte lineage specification that may lead to reduced immunocompetence. Through use of single-cell modalities, we also discovered for the first time, that AHR activation decreased in progenitor cells, at both transcriptomic and protein levels, the expression of BCL11A and IRF8 which are critical genes involved in hematopoietic lineage specification processes. Our in vitro model of hematopoiesis, coupled with single cell tools, therefore allows for a better understanding of the role played by AHR in modulating hematopoietic differentiation.

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Section: Introductionmentioning

confidence: 99%

An in vitro model of human hematopoiesis identifies a regulatory role for the aryl hydrocarbon receptor

et al. 2023

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In Vitro Human Haematopoietic Stem Cell Expansion and Differentiation

Bozhilov

Hsu

Brown

et al. 2023

Cells

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The haematopoietic system plays an essential role in our health and survival. It is comprised of a range of mature blood and immune cell types, including oxygen-carrying erythrocytes, platelet-producing megakaryocytes and infection-fighting myeloid and lymphoid cells. Self-renewing multipotent haematopoietic stem cells (HSCs) and a range of intermediate haematopoietic progenitor cell types differentiate into these mature cell types to continuously support haematopoietic system homeostasis throughout life. This process of haematopoiesis is tightly regulated in vivo and primarily takes place in the bone marrow. Over the years, a range of in vitro culture systems have been developed, either to expand haematopoietic stem and progenitor cells or to differentiate them into the various haematopoietic lineages, based on the use of recombinant cytokines, co-culture systems and/or small molecules. These approaches provide important tractable models to study human haematopoiesis in vitro. Additionally, haematopoietic cell culture systems are being developed and clinical tested as a source of cell products for transplantation and transfusion medicine. This review discusses the in vitro culture protocols for human HSC expansion and differentiation, and summarises the key factors involved in these biological processes.

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Pan-myeloid Differentiation of Human Cord Blood Derived CD34<sup>+</sup> Hematopoietic Stem and Progenitor Cells

Cited by 2 publications

References 0 publications

An in vitro model of human hematopoiesis identifies a regulatory role for the aryl hydrocarbon receptor

An in vitro model of human hematopoiesis identifies a regulatory role for the aryl hydrocarbon receptor

In Vitro Human Haematopoietic Stem Cell Expansion and Differentiation

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