PAN GU: a protein kinase that inhibits S phase and promotes mitosis in early <i>Drosophila</i> development

Fenger, Douglas D.; Carminati, Janet L.; Burney-Sigman, Deborah L.; Kashevsky, Helena; Dines, Jessica L.; Elfring, Lisa; Orr‐Weaver, Terry L.

doi:10.1242/dev.127.22.4763

Cited by 55 publications

(11 citation statements)

References 38 publications

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“…2017). Embryos with mutations in PNG subunits arrest in early development with failures in nuclear division resulting from the failure to translate cyclin B (Shamanski and Orr-Weaver 1991; Fenger et al . 2000; Lee et al .…”

Section: Resultsmentioning

confidence: 99%

“…2000), Wor-Gal4; tub-Gal80 ts (Komori et al . 2014), y png [50] w/FM6 (Fenger et al . 2000), plu [1] /CyO (Shamanski and Orr-Weaver 1991), plu [3] /CyO (Shamanski and Orr-Weaver 1991), brat 150 , FRT40A (Betschinger et al .…”

Section: Methodsmentioning

confidence: 99%

“…PNG is comprised of three subunits (Pangu (PNG), Giant nuclei (GNU) and Plutonium (PLU)) and is subject to precise control such that it is active in a short window following egg activation (Hara et al 2017). Embryos with mutations in PNG subunits arrest in early development with failures in nuclear division resulting from the failure to translate cyclin B (Shamanski and Orr-Weaver 1991;Fenger et al 2000;Lee et al 2003;Vardy and Orr-Weaver 2007). We performed immunoblots for ZLD in embryos lacking functional PNG subunits, PNG or PLU.…”

Section: Zld Levels Depend On the Pan Gu Kinasementioning

confidence: 99%

“…All stocks were grown on molasses food at 25°C. Fly strains used in this study: w 1118 , twe HB5 cn bw/CyO (Courtot et al 1992), Elav-Gal4 hs-flp, UAS-mCD8::GFP (BDSC#5146), tub-Gal80, FRT40A (BDSC#5192), FRT40A (Lee et al 2000), Wor-Gal4; tub-Gal80 ts (Komori et al 2014), y png [50] w/FM6 (Fenger et al 2000), plu [1] /CyO (Shamanski and Orr-Weaver 1991), plu [3] /CyO (Shamanski and Orr-Weaver 1991), brat 150 , FRT40A (Betschinger et al 2006), brat Df(2L)TE37C-7 /CyO (Stathakis et al 1995), brat fs1 /CyO (Schüpbach and Wieschaus 1991), and His2Av-RFP(III) (BDSC#23650).…”

Section: Drosophila Strains and Geneticsmentioning

confidence: 99%

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Premature translation of the zygotic genome activator Zelda is not sufficient to precociously activate gene expression

Larson

Komori

Fitzpatrick

et al. 2022

Preprint

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Following fertilization, the unified germ cells rapidly transition to a totipotent embryo. Maternally deposited mRNAs encode the proteins necessary for this reprogramming as the zygotic genome remains transcriptionally quiescent during the initial stages of development. The transcription factors required to activate the zygotic genome are among these maternally deposited mRNAs and are robustly translated following fertilization. In Drosophila, the mRNA encoding Zelda, the major activator of the zygotic genome, is not translated until one hour after fertilization. Here we demonstrate that zelda translation is repressed following fertilization by the TRIM-NHL protein Brain tumor (BRAT). BRAT also regulates Zelda levels in the larval neuroblast lineage. In the embryo, BRAT-mediated translational repression is regulated by the Pan Gu (PNG) kinase, which is triggered by egg activation. The PNG kinase phosphorylates translational regulators, suggesting that PNG kinase activity alleviates translational repression of zelda by BRAT and coupling translation of zelda with that of other regulators of early embryonic development. Using the premature translation of zelda in embryos lacking BRAT activity, we showed that early translation of a zygotic genome activator is not sufficient to drive precocious gene expression. Instead, Zelda-target genes showed increased expression at the time they are normally activated. We propose that transition through early development requires the integration of multiple processes, including the slowing of the nuclear division cycle and activation of the zygotic genome. These processes are coordinately controlled by PNG kinase-mediated regulation of translation.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Zld Levels Depend On the Pan Gu Kinasementioning

confidence: 99%

Section: Drosophila Strains and Geneticsmentioning

confidence: 99%

See 2 more Smart Citations

Premature translation of the zygotic genome activator Zelda is not sufficient to precociously activate gene expression

Larson

Komori

Fitzpatrick

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…All stocks were grown on molasses food at 25°C. Fly strains used in this study: w 1118 , twe HB5 cn bw/CyO ( Courtot et al 1992 ) , Elav-Gal4 hs-flp, UAS-mCD8::GFP (BDSC#5146), tub-Gal80, FRT40A (BDSC#5192), FRT40A ( Lee et al 2000 ), Wor-Gal4; tub-Gal80 ts ( Komori et al 2014 ), y png [50] w/FM6 ( Fenger et al 2000 ), plu [1] /CyO ( Shamanski and Orr-Weaver 1991 ), plu [3] /CyO ( Shamanski and Orr-Weaver 1991 ), brat 150 , FRT40A ( Betschinger et al 2006 ) , brat Df(2L)TE37C-7 /CyO ( Stathakis et al 1995 ), brat fs1 /CyO ( Schüpbach and Wieschaus 1991 ), and His2Av-RFP(III) (BDSC#23650). brat 150 clones were generated using larvae that were cultured at 25°C after larval hatching.…”

Section: Methodsmentioning

confidence: 99%

Premature translation of the Drosophila zygotic genome activator Zelda is not sufficient to precociously activate gene expression

Larson

Komori

Fitzpatrick

et al. 2022

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

Following fertilization, the unified germ cells rapidly transition to a totipotent embryo. Maternally deposited mRNAs encode the proteins necessary for this reprogramming as the zygotic genome remains transcriptionally quiescent during the initial stages of development. The transcription factors required to activate the zygotic genome are among these maternally deposited mRNAs and are robustly translated following fertilization. In Drosophila, the mRNA encoding Zelda, the major activator of the zygotic genome, is not translated until one hour after fertilization. Here we demonstrate that zelda translation is repressed in the early embryo by the TRIM-NHL protein Brain tumor (BRAT). BRAT also regulates Zelda levels in the larval neuroblast lineage. In the embryo, BRAT-mediated translational repression is regulated by the Pan Gu (PNG) kinase, which is triggered by egg activation. The PNG kinase phosphorylates translational regulators, suggesting that PNG kinase activity alleviates translational repression of zelda by BRAT and coupling translation of zelda with that of other regulators of early embryonic development. Using the premature translation of zelda in embryos lacking BRAT activity, we showed that early translation of a zygotic genome activator is not sufficient to drive precocious gene expression. Instead, Zelda-target genes showed increased expression at the time they are normally activated. We propose that transition through early development requires the integration of multiple processes, including the slowing of the nuclear division cycle and activation of the zygotic genome. These processes are coordinately controlled by PNG kinase-mediated regulation of translation.

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Cytostatic Arrest: Post‐Ovulation Arrest until Fertilization in Metazoan Oocytes

2010

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PAN GU: a protein kinase that inhibits S phase and promotes mitosis in early Drosophila development

Cited by 55 publications

References 38 publications

Premature translation of the zygotic genome activator Zelda is not sufficient to precociously activate gene expression

Premature translation of the zygotic genome activator Zelda is not sufficient to precociously activate gene expression

Premature translation of the Drosophila zygotic genome activator Zelda is not sufficient to precociously activate gene expression

Cytostatic Arrest: Post‐Ovulation Arrest until Fertilization in Metazoan Oocytes

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