Pan-European monitoring of susceptibility to human-use antimicrobial agents in enteric bacteria isolated from healthy food-producing animals

Jong, Anno de; Thomas, Valérie; Simjee, Shabbir; Godinho, Kevin; Schiessl, B.; Klein, U.; Butty, Pascal; Vallé, Michel; Marion, Hervé; Shryock, Thomas R.

doi:10.1093/jac/dkr539

Cited by 67 publications

(67 citation statements)

References 22 publications

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“…In this study, 8 different antibiotics available in the market were used to study antibiogram profiles of the E. coli and Salmonella spp. isolated from diarrhoeic cattle by disc diffusion method and the results were supported by Akond et al, 2009;Wouafo et al, 2010;Hyeon et al, 2011;De et al, 2012;Jeyasanta et al, 2012;Jahan et al, 2013. Due to financial crisis and limited time following further studies like collection of more samples covering many areas and farms of Bangladesh to get more precise information, molecular detection of shiga toxin producing E. coli, molecular characterization of the isolated bacterial species by pulsed field gel electrophoresis (PFGE), 16S rRNA gene sequencing of the isolated E. coli and Salmonella spp., serotyping, multiplex PCR of the isolated Salmonella spp., pathogenicity study would not be possible.…”

Section: Discussionsupporting

confidence: 52%

Isolation, molecular identification and antibiogram profiles of Escherichia coli and Salmonella spp. from diarrhoeic cattle reared in selected areas of Bangladesh

Sohidullah

Khan

Islam

et al. 2017

Asian J. Med. Biol. Res

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The present research work was undertaken to find out the passive causes of occurrences of diarrhoea in terms of age, sex, season and location differences through isolation and identification of the E. coli and Salmonella spp. using cultural, biochemical and molecular from the field samples of the diarrhoeic cattle and to study the antibiogram profiles of the isolated bacterial species. Considering above purposes, a total of 57 rectal swab samples were collected from the diarrhoeic cattle of Mymensingh sadar, Trishal, Valuka, Natore sadar and Gomostapur, Chapai Nawabganj. Different types of cultural media like Nutrient agar, MacConkey`s (MC) agar, Eosin Methylene Blue (EMB) agar, Salmonella-Shigella (SS) agar, Xylose-Lysine-Deoxycholate (XLD) agar and Blood agar were used to isolate and to study the cultural properties of the E. coli and Salmonella spp. Finally Gram's staining and different biochemical tests were performed to identify those two bacterial species. Out of 57 samples, 27 were positive for E. coli and 8 were positive for Salmonella spp. On the basis of information from cattle owners and clinical signs the prevalence of diarrhoea was recorded as 30.99% and the pvalue was calculated as 0.001 (p<0.01) which was noted as highly significant. The prevalence percentages of the E. coli and Salmonella spp. were differed depending on different epidemiological parameters like age, sex, season and location. Moreover, the molecular identifications were further confirmed by means of PCR assay using specific primers for E. coli and Salmonella spp. This was done targeting 16S rRNA gene where they were found to be positive showing amplification of 585 bp for E. coli and 574 bp for Salmonella spp. From the study of the antibiogram profiles, it was revealed that E. coli were susceptible to ciprofloxacin, gentamicin and norfloxacin but resistant to tetracycline, erythromycin, amoxicillin and streptomycin whereas Salmonella spp. were susceptible to ciprofloxacin, gentamicin, amoxicillin and streptomycin but resistant to azithromycin, tetracycline and erythromycin. The findings of this research work would certainly help to select the proper antibiotics against diarrhoea in cattle of Bangladesh and to overcome the multi-drug resistant problem of the bacteria.

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Section: Discussionsupporting

confidence: 52%

Isolation, molecular identification and antibiogram profiles of Escherichia coli and Salmonella spp. from diarrhoeic cattle reared in selected areas of Bangladesh

Sohidullah

Khan

Islam

et al. 2017

Asian J. Med. Biol. Res

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show abstract

“…This result were similar to previous findings where Salmonella spp. sensitive to Chloramphenicol supported by [30,31,37,38,39]. The result of this test provided the guideline for the veterinarian to select appropriate antibiotics to reduce the economic loss through selecting the sensitive antibiotics for Salmonellosis which have public health significance.…”

Section: Resultsmentioning

confidence: 99%

Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh

2016

J App Biol Biotech

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The objectives of this study were to isolate the associated Salmonella spp. from cloacal swabs of broiler and their antibiogram studies. A total of 80 cloacal swabs comprising of 50 samples of apparently healthy broiler and 30 samples of diarrheic broiler were collected from different poultry farms at Sylhet, Bangladesh during Ja nuary to June 2013. The samples were subjected for isolation and identification of Salmonella spp. through a series of conventional bacteriological studies like study of morphology, staining properties, and biochemical characteristics. In results, 48% (n= 24/50) swab samples of healthy broiler and 66.7% (n= 20/30) diarrheic broiler were found to be associated with Salmonella spp. Among the 44 positive Salmonella isolates 47.73% (n=21) were Salmonella pullorum, 36.36% (n=16) isolates were Salmonella gallinarum and the rest 15.91% (n=7) isolates were Salmonella typhimurium. Sensitivity test was conducted against 10 commonly used antibiotics, of which Penicillin-G, Erythromycin, Ampicillin, and Bacitracin were found to be resistant, and Ceftriaxone, Gentamycin, and Choloramphenicol showed considerably better sensitivity as compared to others. It is concluded that Salmonella spp. are present in broiler, and the bacteria can be clinically controlled by using Ceftriaxone, Gentamycin, and Choloramphenicol.

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“…The distributions are significantly different between these four countries (Kruskal-Wallis P < 1.07 3 10 À5 ). (Goossens et al 2005) measured in defined daily doses (DDDs) per 1000 inhabitants, frequency of antibiotic resistance in bacterial isolates from slaughterhouses in a 2011 comparative study (de Jong et al 2012), and fraction of approximately 1000 respondents in each country that had taken antibiotics during the last 12 mo (Borg 2012). Resistance potential correlates significantly with outpatient antibiotic use (Pearson r = 0.97; t-test [N = 4, df = 2]; Bonferroni-corrected P < 0.08).…”

Section: Discussionmentioning

confidence: 99%

Country-specific antibiotic use practices impact the human gut resistome

et al. 2013

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Despite increasing concerns over inappropriate use of antibiotics in medicine and food production, population-level resistance transfer into the human gut microbiota has not been demonstrated beyond individual case studies. To determine the ''antibiotic resistance potential'' for entire microbial communities, we employ metagenomic data and quantify the totality of known resistance genes in each community (its resistome) for 68 classes and subclasses of antibiotics. In 252 fecal metagenomes from three countries, we show that the most abundant resistance determinants are those for antibiotics also used in animals and for antibiotics that have been available longer. Resistance genes are also more abundant in samples from Spain, Italy, and France than from Denmark, the United States, or Japan. Where comparable country-level data on antibiotic use in both humans and animals are available, differences in these statistics match the observed resistance potential differences. The results are robust over time as the antibiotic resistance determinants of individuals persist in the human gut flora for at least a year.

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Pan-European monitoring of susceptibility to human-use antimicrobial agents in enteric bacteria isolated from healthy food-producing animals

Cited by 67 publications

References 22 publications

Isolation, molecular identification and antibiogram profiles of Escherichia coli and Salmonella spp. from diarrhoeic cattle reared in selected areas of Bangladesh

Isolation, molecular identification and antibiogram profiles of Escherichia coli and Salmonella spp. from diarrhoeic cattle reared in selected areas of Bangladesh

Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh

Country-specific antibiotic use practices impact the human gut resistome

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