Pan-drug-resistant Pseudomonas aeruginosa causing nosocomial infection at a university hospital in Taiwan

Abstract

This study evaluated the clinical and microbiological characteristics of 16 patients who were colonised or infected with 26 isolates of pan-drug-resistant Pseudomonas aeruginosa (PDRPA; intermediately-resistant or resistant to all cephalosporins, piperacillin-tazobactam, aztreonam, carbapenems, ciprofloxacin and aminoglycosides) in a university hospital during 1999-2002. All the isolates had colistin MICs < or = 4 mg/L, 19 (73%) isolates had bla(VIM-3), and 25 (96%) isolates had class I integrons (intI). Time-kill studies for two PDRPA blood isolates demonstrated synergism for cefepime-amikacin after 24 h. Pulsed-field gel electrophoresis analysis of the isolates revealed a polyclonal nature (12 pulsotypes), although clonal dissemination of PDRPA isolates among these patients was also present.

Toluidine blue O photodynamic inactivation on multidrug‐resistant pseudomonas aeruginosa

“…A total of 60 MDR P. aeruginosa strains (14 strains in the period from 1999 to 2002 [3], 46 from 2003 to 2004) collected from National Taiwan University Hospital, a 2,500-bed hospital located in Northern Taiwan were used in this study. The MDR strains were recovered from sputum (n ¼ 43), blood (n ¼ 3), wound pus (n ¼ 4), urine (n ¼ 6), and others (n ¼ 4).…”

“…Infections caused by multidrug-resistant (MDR) Pseudomonas aeruginosa are emerging problems, especially those caused by strains resistant to carbapenems [1][2][3][4]. Very few antimicrobial agents are available for the treatment of MDR P. aeruginosa infections.…”

Tn 6001 , a Transposon-Like Element Containing the bla VIM-3 -Harboring Integron In450

“…Pseudomonas aeruginosa has caused serious problems in many countries, including Taiwan (5,7,10). Resistance to carbapenems in MDR P. aeruginosa strains is a major concern.…”

“…Resistance to carbapenems in MDR P. aeruginosa strains is a major concern. Resistance to carbapenems in P. aeruginosa is usually associated with impermeability that arises via the loss of the oprD-encoded porin, active efflux pump, and/or the production of metallo-␤-lactamases (MBLs), such as the IMP-and VIM-type MBLs (5,6,19,20,22).…”

Epinecidin-1 Has Immunomodulatory Effects, Facilitating Its Therapeutic Use in a Mouse Model of Pseudomonas aeruginosa Sepsis

“…The opportunistic Gram-negative bacterium Pseudomonas aeruginosa is a frighteningly dangerous pathogen that can give rise to both localized and systemic infections, including burn wound infections, pneumonia, abdominal infections, chronic ulcers, cystic fibrosis, and sepsis (2)(3)(4)(5). There have been several outbreaks of MDR or even panresistant P. aeruginosa worldwide, and these have been associated with high morbidity and mortality (6). Strains of P. aeruginosa have developed resistance to many antibiotics, including imipenem, cephalosporins, piperacillin-tazobactam, aztreonam, carbapenems, ciprofloxacin, and aminoglycosides (7,8).…”