PAMAM-pullulan conjugates as targeted gene carriers for liver cell

Askarian, Saeedeh; Abnous, Khalil; Ayatollahi, Sara; Farzad, Sara Amel; Oskuee, Reza Kazemi; Ramezani, Mohammad

doi:10.1016/j.carbpol.2016.10.030

Cited by 36 publications

(7 citation statements)

References 45 publications

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“…By adding PEI to the pDNA/EtBr solution and condensation of pDNA by PEI, because of the release of some accommodated EtBrs molecules from pDNA and negligible interactions between them (because of the structural transformation of pDNA from a helix to a globular form), fluorescence intensity decreased. [90][91][92][93] Moreover, the addition of the aptamer did not have any effect on the fluorescence and shape of the curves. Particle size and zeta potential were additional measurable features in this study.…”

Section: Targeted Gene Delivery By Aptamer-pei Nanocomplexesmentioning

confidence: 86%

<p>Aptamer Hybrid Nanocomplexes as Targeting Components for Antibiotic/Gene Delivery Systems and Diagnostics: A Review</p>

Rabiee¹,

Ahmadi²,

Arab³

et al. 2020

IJN

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With the passage of time and more advanced societies, there is a greater emergence and incidence of disease and necessity for improved treatments. In this respect, nowadays, aptamers, with their better efficiency at diagnosing and treating diseases than antibodies, are at the center of attention. Here, in this review, we first investigate aptamer function in various fields (such as the detection and remedy of pathogens, modification of nanoparticles, antibiotic delivery and gene delivery). Then, we present aptamer-conjugated nanocomplexes as the main and efficient factor in gene delivery. Finally, we focus on the targeted co-delivery of genes and drugs by nanocomplexes, as a new exciting approach for cancer treatment in the decades ahead to meet our growing societal needs.

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Section: Targeted Gene Delivery By Aptamer-pei Nanocomplexesmentioning

confidence: 86%

<p>Aptamer Hybrid Nanocomplexes as Targeting Components for Antibiotic/Gene Delivery Systems and Diagnostics: A Review</p>

Rabiee¹,

Ahmadi²,

Arab³

et al. 2020

IJN

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“…This agent could condense DNA and deliver a dye-labeled pDNA in human hepatoma HepG2 cells. Pullulan conjugation also enhanced the gene delivery efficacy of PAMAM dendrimer in HepG2 cell line [173].…”

Section: Natural Carbohydrate Polymers For Gene Deliverymentioning

confidence: 99%

Biodegradable Polymers for Gene Delivery

2019

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The cellular transport process of DNA is hampered by cell membrane barriers, and hence, a delivery vehicle is essential for realizing the potential benefits of gene therapy to combat a variety of genetic diseases. Virus-based vehicles are effective, although immunogenicity, toxicity and cancer formation are among the major limitations of this approach. Cationic polymers, such as polyethyleneimine are capable of condensing DNA to nanoparticles and facilitate gene delivery. Lack of biodegradation of polymeric gene delivery vehicles poses significant toxicity because of the accumulation of polymers in the tissue. Many attempts have been made to develop biodegradable polymers for gene delivery by modifying existing polymers and/or using natural biodegradable polymers. This review summarizes mechanistic aspects of gene delivery and the development of biodegradable polymers for gene delivery.

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“…7 Poly-L-lysine (PLL), polyethyleneimine (PEI), pullulan, and polyallylamine are the most famous polymers that have been widely employed in this field. [8][9][10][11][12] PLL is a natural cationic polymer with positively charged side chain, due to the presence of amino acid lysine as the repeat unit, this polymer is biodegradable and this property considers it as a remarkable candidate for in vivo applications such as drug delivery and cell tracking. [13][14][15] PLL polymer can act as a vehicle for different cargoes such as drugs, peptides, and genes 16,17 and also as a promising coating agent for improving biocompatibility of different nanoparticles for cell tracking process.…”

Section: Introductionmentioning

confidence: 99%

Evaluation and comparison of cytotoxicity, genotoxicity, and apoptotic effects of poly-l-lysine/plasmid DNA micro- and nanoparticles

et al. 2019

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The principal impediment to gene therapy is the development of efficient, nontoxic gene carriers that can handle and deliver foreign genetic materials into various cell types, including healthy and cancerous cells. Poly-l-lysine (PLL) polymers are one of the most favorable gene carriers among nonviral vectors, and PLL had low transfection and safety issues. The purpose of this study was to measure cellular toxicity, DNA damage, and apoptotic effects of PLL nanoparticles. Neuro2A mammalian cells were cultured and exposed to PLL/DNA complexes at different polymer/DNA ratios ( C/ P ratio 2 and 6) for 24 h. To evaluate metabolic activity, genotoxicity, and apoptotic influences of PLL nanoparticle, the following experimental methods were employed, in order: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), DNA damage (COMET analysis) assay, and sub-G1 peak apoptosis assay. Our data indicate that toxicity is concentration dependent and a high concentration of polymer declined the metabolic activity. In addition, largest complexes ( C/ P 6 in HEPES buffered saline buffer) have slighter negative impact on metabolic activity. In agreement with our cytotoxicity data, apoptotic assay result represented that increase in size of PLL/DNA complexes decrease the number of apoptotic cells. Also, there was a remarkable increase in percent tail DNA of Neuro2A cells treated with higher concentration of PLL and its polyplexes. The present study demonstrated that PLL/DNA complexes caused cytotoxic, apoptotic, and genotoxic effects in a dose-dependent and weight ratio-dependent manner, which also affected the size of polyplexes.

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PAMAM-pullulan conjugates as targeted gene carriers for liver cell

Cited by 36 publications

References 45 publications

<p>Aptamer Hybrid Nanocomplexes as Targeting Components for Antibiotic/Gene Delivery Systems and Diagnostics: A Review</p>

<p>Aptamer Hybrid Nanocomplexes as Targeting Components for Antibiotic/Gene Delivery Systems and Diagnostics: A Review</p>

Biodegradable Polymers for Gene Delivery

Evaluation and comparison of cytotoxicity, genotoxicity, and apoptotic effects of poly-l-lysine/plasmid DNA micro- and nanoparticles

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