PAM, OLA, and LNA are Differentially Taken Up and Trafficked Via Different Metabolic Pathways in Porcine Adipocytes

Yu, Caihua; Xi, Lingling; Chen, Jin; Jiang, Qin; Yi, Hongbo; Wang, Yizhen; Wang, Xinxia

doi:10.1007/s11745-017-4302-x

Cited by 6 publications

(5 citation statements)

References 34 publications

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“…In this study, we found that OA increased the protein expression of the adipogenesis-related gene PPARγ, which promotes FFA uptake into adipocytes. Yu et al have reported that OA and linoleic acid promote lipid accumulation in adipocytes, probably through regulating PPARγ phosphorylation [ 22 ], which is consistent with our results.…”

Section: Discussionsupporting

confidence: 93%

p62 is linked to mitophagy in oleic acid-induced adipogenesis in human adipose-derived stromal cells

et al. 2018

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BackgroundObesity is closely related to the abnormal differentiation of adipocytes, which are subjected to high plasma levels of free fatty acids (FFAs). As the most abundant FFA in the bloodstream, oleic acid (OA) has the ability to induce adipogenic differentiation in human adipose-derived stromal cells (hADSCs). Recently, p62, an autophagy mediator, has been shown to play a role in obesity and adipose tissue metabolism. Therefore, the aim of this study was to investigate the roles of autophagy and mitochondrial function at different stages of OA (in combination with insulin and dexamethasone)-induced adipogenesis in hADSCs.MethodsThe hADSCs were incubated with OA, insulin, and dexamethasone after pretreatment with autophagy inhibitors or knockdown of p62 with shRNA. The adiposeness level was then analyzed by oil red O staining in the cells. The related proteins or mRNA levels were detected by western blot analysis or quantitative real-time polymerase chain reaction (PCR).ResultsTreatment with 80 μM OA (substituted for isobutylmethylxantine; IBMX) for 10 days successfully induced hADSCs to adipocytes. During OA-induced adipogenesis, autophagy was induced, with an increased LC3II/I ratio on day 3 and a decreased protein level of p62 on and after day 3. Inhibition of autophagy with 3-methyladenine (3MA) at the early stage (day 0 to day 3) of differentiation, but not at the middle or late stage, significantly decreased OA-induced adipogenesis; while knockdown of p62 with shRNA significantly promoted adipogenesis in hADSCs. Moreover, the copy number of mtDNA (the ND1 gene) and the protein level of TOM20, a mitochondrial membrane protein, were increased following OA treatment, which was related to the stability of mitochondria. Interestingly, knockdown of p62 increased the mito-LC3II/I and cyto-LC3II/I ratios by 110.1% and 73.3%, respectively. The increase in the ratio of mito-LC3II/I was higher than that of cyto-LC3II/I. Furthermore, p62 knockdown-enhanced adipogenesis in hADSCs was abolished by inhibiting mitophagy with cyclosporine A.ConclusionsThese results suggested that p62 plays a protective role in adipogenesis of hADSCs through regulating mitophagy.

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Section: Discussionsupporting

confidence: 93%

p62 is linked to mitophagy in oleic acid-induced adipogenesis in human adipose-derived stromal cells

et al. 2018

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“…However, high LA diets were correlated with a decrease in p-IKKα/β (relative to other diets) and p-FADD (relative to euthermia), suggesting NFκB signaling is decreased in WAT. High LA diets can induce the expression of other transcription factors such as AP1 that are capable of increasing sod2 gene expression (Miao and St. Clair, 2009;Yu et al, 2017). Therefore, future studies should focus transcription factors other than NFκB to help explain the increase in sod2 expression in WAT from dormice fed a high LA diet.…”

Section: Resultsmentioning

confidence: 99%

The Ratio of Linoleic and Linolenic Acid in the Pre-hibernation Diet Influences NFκB Signaling in Garden Dormice During Torpor

Logan

Watts

Posautz

et al. 2020

Front. Mol. Biosci.

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The fatty acid composition of a pre-hibernation diet can influence the depth and duration of metabolic suppression achieved by hibernators. More specifically, a diet high in n−6 polyunsaturated fatty acids (PUFAs) relative to n−3 PUFAs is essential to maximize torpor expression. However, few studies have investigated how diets with different n−6/n−3 PUFA ratios change stress-inducible cell signaling. Garden dormice (Eliomys quercinus) were fed one of three diets designed with different ratios of n−6 PUFA linoleic acid (LA) and n−3 PUFA linolenic acid (ALA). Then, NFκB signaling was assessed in the white adipose, brown adipose, and liver tissues of euthermic and hibernating dormice via multiplex and RT-qPCR analyses of relative protein and transcript levels, respectively. Dormice fed a high LA diet regulated NFκB signaling in a protective manner in all tissues. NFκB signaling was generally decreased in the high LA group, with significant decreases in the protein levels of NFκB mediators IKKα/β, IκBα, and downstream pro-apoptotic protein FADD. Liver and white adipose from torpid dormice fed a high LA diet increased sod2 expression relative to the other diets or relative to euthermic controls, indicating protection against ROS generated from potentially increased β-oxidation of n−6 PUFAs. The low LA diet increased biomarkers for apoptosis relative to other diets and relative to euthermia, suggesting low LA diets may be detrimental to hibernator health. Overall, this study suggests that changes in the ratio of n−6/ n−3 PUFAs in the diet influences apoptotic and antioxidant responses in white adipose, brown adipose, and liver of hibernating garden dormice.

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“…Turner PA et al considered that linoleic acid supplementation resulted in enhanced adipogenesis (Turner et al, 2018). The study of Yu C et al also suggested Linoleic acid signi cantly promoted lipid accumulation, probably by regulating PPARγ phosphorylation in adipocytes (Yu et al, 2017). As a consequence, excessive accumulation of linoleic acid might exacerbates FHN through increasing adipogenesis.…”

Section: Biomarker Analysismentioning

confidence: 99%

Comparative metabolomic analysis of human cancellous bone reveals the critical role of linoleic acid metabolism in femur head necrosis based on GC-MS

Zhu

Wang

Luo

et al. 2023

Preprint

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Introduction Femur head necrosis (FHN) is a challengeable clinical disease with unclear underlying mechanism and a low rate of early diagnosis. Objectives To elucidate altered metabolism pathways in FHN and identify putative biomarkers for the detection of FHN. Methods we recruited 26 patients with femur head necrosis and 22 patients with femur neck fracture in this study. Cancellous bone tissues from the femoral heads were collected after the surgery and were analyzed using an untargeted metabolomics approach on the basis of gas chromatography-mass spectrometry (GC-MS). Results The resulting data were analyzed via uni- and multivariate statistical approaches, and we identified 38 metabolites distinctly changed in FHN group. Among them, 33 metabolites were upregulated and 5 were downregulated in FHN. The pathway analysis results showed that linoleic acid metabolism were the most relevant to FHN pathology. On the basis of metabolites network, L-lysine, L-glutamine and L-serine were deemed as the junctions of the whole metabolites. At last, we figured out that 9,12-octadecadienoic acid, inosine, L-proline and octadecanoic acid can be considered as the potential biomarkers of FHN. Conclusion Our study provided a new insight into the pathogenesis of FHN and identified 4 biomarkers in FHN. Linoleic acid metabolism could be considered as the core in FHN and 9,12-Octadecadienoic acid could be considered as the diagnostic marker.

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PAM, OLA, and LNA are Differentially Taken Up and Trafficked Via Different Metabolic Pathways in Porcine Adipocytes

Cited by 6 publications

References 34 publications

p62 is linked to mitophagy in oleic acid-induced adipogenesis in human adipose-derived stromal cells

p62 is linked to mitophagy in oleic acid-induced adipogenesis in human adipose-derived stromal cells

The Ratio of Linoleic and Linolenic Acid in the Pre-hibernation Diet Influences NFκB Signaling in Garden Dormice During Torpor

Comparative metabolomic analysis of human cancellous bone reveals the critical role of linoleic acid metabolism in femur head necrosis based on GC-MS

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