Palytoxin Acts on Na+,K+-ATPase but not Nongastric H+,K+-ATPase

Guennoun-Lehmann, Saida; Fonseca, James; Horisberger, Jean‐Daniel; Rakowski, Robert F.

doi:10.1007/s00232-007-9040-1

Cited by 9 publications

(5 citation statements)

References 40 publications

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“…It is a safe and reliable alternative to radioactive tracer flux assays, and allows addressing a large scope of experimental questions within considerably short time. In accordance, only very limited data have been acquired with radiotracers [24][25][26][27] . The AAS method is particularly useful for H + ,K + -ATPase, which -due to its electroneutral transport activity -cannot be analyzed by standard electrophysiology.…”

Section: Temperature Dependence Of Rb + Fluxesmentioning

confidence: 99%

Measuring Cation Transport by Na,K- and H,K-ATPase in <em>Xenopus</em> Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Dürr

Tavraz

Spiller

et al. 2013

JoVE

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Section: Temperature Dependence Of Rb + Fluxesmentioning

confidence: 99%

Measuring Cation Transport by Na,K- and H,K-ATPase in <em>Xenopus</em> Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Dürr

Tavraz

Spiller

et al. 2013

JoVE

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“…Vanadate is an effective inhibitor in ionic conditions promoting the E2 conformation, while the inhibitory effect of PCALC36 is equal in ionic conditions favoring either the E1 or E2 conformation (51). One interesting finding is that palytoxin, which binds to the Na + K + ‐APTase from the extracellular face, transforms the pump into a cation channel by allowing the conformation in which both gates are opened that is not present in the native state of pump (52).…”

Section: Inhibitors and Their Mechanism Of Actionmentioning

confidence: 99%

“…SK&F97574 [3‐Butryl‐4‐(2‐methylamino)‐8‐(2‐hydroxyethoxy) quinoline] is also a potent, reversible inhibitor of the gastric H + K + ‐ATPase. Duration of action of SK&F 97574 is longer than that of the histamine H2 receptor (H2RAs) antagonists such as cimetidine but shorter than that of covalent H + K + ‐inhibitors such as omeprazole (52,59). Consequent covalent inhibition of H + K + ‐ATPase blocks the final step of acid secretion; hence, the PPIs omeprazole, lansoprazole, and pantoprazole are more effective than H2RAs antagonists in controlling acid secretion.…”

Section: Inhibitors and Their Mechanism Of Actionmentioning

confidence: 99%

The Nucleotide, Inhibitor, and Cation Binding Sites of P‐type II ATPases

Chourasia

Sastry

2012

Chem Biol Drug Des

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P-type ATPases constitute a ubiquitous superfamily of cation transport enzymes, responsible for carrying out actions of paramount importance in biology such as ion transport and expulsion of toxic ions from cells. The harmonized toggling of gates in the extra-and intracellular domains explain the phenomenon of specific cation binding in selective physiological states. A quantitative understanding of the fundamental aspects of ion transport mechanism and regulation of P-type ATPases requires detailed knowledge of thermodynamical, structural, and functional properties. Computational studies have made significant contributions to our understanding of biological ion pumps. Various 3D structures of Ca 2+ -ATPase between E1 and E2 transition states have given a impetus to the theorists to work on the Na + K + -and H + K + -ATPase to address important questions about their function. The current review delineates the importance of cation, nucleotide, and inhibitor binding domains, with a focus on the therapeutic potential and biological relevance of the three P-type II ATPases. This will give an insight into the ion selectivity and their conduction across the transmembrane helices of P-type II ATPases, which may pave the way to a range of fundamental questions about the mechanism and aid in the efforts of structure-and analog-based drug design.

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“…In the system evaluated in Rohlfshagen and Bryson (2008) (see Figure 7) the agents had four goals. Two high-priority goals have to do with the immediate health of the agents: eating and drinking; while two have to do with the adaptive well-being of the agents: grooming (which correlates to safety from predation, see Lehmann & Bryson, 2007) and exploring. For the grooming goal, for example, consummation is the act of grooming, while preparatory actions include selecting and approaching a partner.…”

Section: Flexible Latchingmentioning

confidence: 99%

Simplifying the Design of Human-Like Behaviour

Bryson

Tanguy

2010

International Journal of Synthetic Emotions

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Human intelligence requires decades of full-time training before it can be reliably utilized in modern economies. In contrast, AI agents must be made reliable but interesting in relatively short order. Realistic emotion representations are one way to ensure that even relatively simple specifications of agent behavior will be expressed with engaging variation, and those social and temporal contexts can be tracked and responded to appropriately. We describe a representation system for maintaining an interacting set of durative states to replicate emotional control. Our model, the Dynamic Emotion Representation (DER), integrates emotional responses and keeps track of emotion intensities changing over time. The developer can specify an interacting network of emotional states with appropriate onsets, sustains, and decays. The levels of these states can be used as input for action selection, including emotional expression. We present both a general representational framework and a specific instance of a DER network constructed for a virtual character. The character’s DER uses three types of emotional state as classified by duration timescales, keeping with current emotional theory. We demonstrate the system with a virtual actor. We also demonstrate how even a simplified version of this representation can improve goal arbitration in autonomous agents.

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Palytoxin Acts on Na+,K+-ATPase but not Nongastric H+,K+-ATPase

Abstract: Palytoxin (PTX) opens a pathway for ions to pass through Na,K-ATPase. We investigate here whether PTX also acts on non

Cited by 9 publications

References 40 publications

Measuring Cation Transport by Na,K- and H,K-ATPase in <em>Xenopus</em> Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Measuring Cation Transport by Na,K- and H,K-ATPase in <em>Xenopus</em> Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

The Nucleotide, Inhibitor, and Cation Binding Sites of P‐type II ATPases

Simplifying the Design of Human-Like Behaviour

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