Measuring Cation Transport by Na,K- and H,K-ATPase in <em>Xenopus</em> Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Abstract

Whereas cation transport by the electrogenic membrane transporter Na + ,K +

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization

“…On heterologous expression of the retinal Na/K-ATPase, Rb + uptake by oocytes increased approximately sixfold compared with untransfected oocytes ( Figure 3 ). The average uptake was 7 ng Rb + per transfected oocyte in 3 min (corresponding to 7899 nC), which is in line with published results for Na/K-ATPase activity in transfected oocytes ( Dürr et al. , 2013 ).…”

“…, 1998 ; Koenderink et al. , 2000 ; Dürr et al. , 2013 ; Spiller and Friedrich, 2014 ), we used an ouabain-resistant ATP1A3 protein with an half-maximal inhibitory concentration (IC 50 ) in the millimolar range ( Price and Lingrel, 1988 ; Dürr et al.…”

“…, 1972 ; Beauge and Glynn, 1979 ). Intracellular Rb + was determined via atomic absorption spectrophotometry ( Dürr et al. , 2013 ).…”

Mechanisms of cell uptake and toxicity of the anticancer drug cisplatin

“…19 The cDNAs of human Na + ,K + -ATPase a2-and b1-subunit were subcloned into an appropriate expression vector (pcDNA3.1X). 20 To distinguish the activity of the overexpressed human Na + ,K + -ATPase from the endogenous Na + ,K + -ATPase of the oocytes, the mutations Q116R and N127D were introduced into the a2-subunit to obtain an ouabain-resistant protein with an IC50 in the millimolar range.…”

Disruption of ankyrin B and caveolin-1 interaction sites alters Na+,K+-ATPase lateral diffusion in HEK293 cell plasma membranes

“…From these homogenates, 20 µl samples were injected into the graphite furnace for analysis using the autosampler of the AAS instrument. More details can be found in (Dürr et al, 2013;Friedrich, 2015).…”