Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases.

Jones, Aubrey; Davies, H. Maelor; Voelker, Toni A.

doi:10.1105/tpc.7.3.359

Cited by 283 publications

(267 citation statements)

References 38 publications

(69 reference statements)

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“…Compared with the results of function analysis of CnFatB1, CocoFatB1 is specific not only towards 14 : 0-ACP and 16 : 0-ACP, which have been demonstrated in E. coli by Jing et al (2011), but also showed specificity to 18 : 0-ACP in plant. These results are similar to those previously reported for FatB thioesterases from other plants, such as Elaeis guineensis (Othman et al 2000), Jatropha curcas L. (Wu et al 2009), Cuphea hookeriana (Jones et al 1995), Diploknema (Madhuca) butyracea (Jha et al 2006) and Indian mustard (Brassica juncea L. Czern.…”

Section: Discussionsupporting

confidence: 91%

“…Based on amino acid sequence alignments, these enzymes have been functionally characterised and classified into two general families, termed FatA and FatB (Jones et al 1995). All FatAs are orthologous in different species, with the highest activities towards oleoyl-ACP (18 : 1~9-ACP) (Hitr and Yadav 1992;Sánchez-García et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…All FatAs are orthologous in different species, with the highest activities towards oleoyl-ACP (18 : 1~9-ACP) (Hitr and Yadav 1992;Sánchez-García et al 2010). In contrast with the high level of conservation in the specificity of FatAs, FatBs primarily hydrolyse saturated acylACPs with chain lengths that contain between 8 and 18 carbons (Voelker and Davies 1994;Jones et al 1995;Jha et al 2010). The first FatB gene was isolated from the developing seeds of California Bay Tree (Umbellularia californica), and the strong preference of the enzyme for 12 : 0-ACP was verified in Arabidopsis thaliana (L.) Heynh.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and characterisation of an acyl carrier protein thioesterase gene (CocoFatB1) expressed in the endosperm of coconut (Cocos nucifera) and its heterologous expression in Nicotiana tabacum to engineer the accumulation of different fatty acids

Yang

Chen

Liang

et al. 2014

Functional Plant Biol.

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Abstract. Coconut (Cocos nucifera L.) contains large amounts of medium chain fatty acids, which mostly recognise acylacyl carrier protein (ACP) thioesterases that hydrolyse acyl-ACP into free fatty acids to terminate acyl chain elongation during fatty acid biosynthesis. A full-length cDNA of an acyl-ACP thioesterase, designated CocoFatB1, was isolated from cDNA libraries prepared from coconut endosperm during fruit development. The gene contained an open reading frame of 1254 bp, encoding a 417-amino acid protein. The amino acid sequence of the CocoFatB1 protein showed 100% and 95% sequence similarity to CnFatB1 and oil palm (Elaeis guineensis Jacq.) acyl-ACP thioesterases, respectively. Real-time fluorescent quantitative PCR analysis indicated that the CocoFatB1 transcript was most abundant in the endosperm from 8-month-old coconuts; the leaves and endosperm from 15-month-old coconuts had~80% and~10% of this level. The CocoFatB1 coding region was overexpressed in tobacco (Nicotiana tabacum L.) under the control of the seed-specific napin promoter following Agrobacterium tumefaciens-mediated transformation. CocoFatB1 transcript expression varied 20-fold between different transgenic plants, with 21 plants exhibiting detectable levels of CocoFatB1 expression. Analysis of the fatty acid composition of transgenic tobacco seeds showed that the levels of myristic acid (14 : 0), palmitic acid (16 : 0) and stearic acid (18 : 0) were increased by 25%, 34% and 17%, respectively, compared with untransformed plants. These results indicated that CocoFatB1 acts specifically on 14 : 0-ACP, 16 : 0-ACP and 18 : 0-ACP, and can increase medium chain saturated fatty acids. The gene may valuable for engineering fatty acid metabolism in crop improvement programmes.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and characterisation of an acyl carrier protein thioesterase gene (CocoFatB1) expressed in the endosperm of coconut (Cocos nucifera) and its heterologous expression in Nicotiana tabacum to engineer the accumulation of different fatty acids

Yang

Chen

Liang

et al. 2014

Functional Plant Biol.

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“…Total RNA from B. napus cotyledons and developing seeds was isolated as described by Jones et al (1995).…”

Section: Rna Isolationmentioning

confidence: 99%

ACX3, a Novel Medium-Chain Acyl-Coenzyme A Oxidase from Arabidopsis

Froman¹,

Edwards²,

Bursch³

et al. 2000

Plant Physiology

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In a database search for homologs of acyl-coenzyme A oxidases (ACX) in Arabidopsis, we identified a partial genomic sequence encoding an apparently novel member of this gene family. Using this sequence information we then isolated the corresponding full-length cDNA from etiolated Arabidopsis cotyledons and have characterized the encoded recombinant protein. The polypeptide contains 675 amino acids. The 34 residues at the amino terminus have sequence similarity to the peroxisomal targeting signal 2 of glyoxysomal proteins, including the R-[I/Q/L]-X5-HL-XL-X15-22-C consensus sequence, suggesting a possible microsomal localization. Affinity purification of the encoded recombinant protein expressed in Escherichia coli followed by enzymatic assay, showed that this enzyme is active on C8:0-to C14:0-coenzyme A with maximal activity on C12:0-coenzyme A, indicating that it has medium-chain-specific activity. These data indicate that the protein reported here is different from previously characterized classes of ACX1, ACX2, and short-chain ACX (SACX), both in sequence and substrate chain-length specificity profile. We therefore, designate this new gene AtACX3. The temporal and spatial expression patterns of AtACX3 during development and in various tissues were similar to those of the AtSACX and other genes expressed in glyoxysomes. Currently available database information indicates that AtACX3 is present as a single copy gene.

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“…It appears that the synthesis of a particular UFA may require only one additional enzymatic reaction to that of primary lipid synthesis and that all enzymes identified to date that are involved in UFA synthesis are structurally related to enzymes of primary metabolism. Therefore a process of duplication and specialisation of housekeeping genes has given rise to enzymes which have variant substrate preferences that catalyse closely related but modified reactions [9].…”

Section: Engineering Unusual Fatty Acid Production In Rapeseedmentioning

confidence: 99%

Production of unusual fatty acids in rapeseed

Roscoe

Maisonneuve

Delseny

2002

OCL

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Résumé : Les manipulations sur la qualité de l'huile chez les plantes cultivées sont d'un intérêt majeur et dépendent des modifications de la composition en huile des graines. Les acides gras habituels sont stockés sous forme de triacylglycérols. De nombreuses espèces accumulent des acides gras inhabituels (AGI) qui peuvent servir comme précurseurs à de nombreux produits chimiques ou encore conférer de nouvelles propriétés physiques ou chimiques aux huiles. L'isolement de gènes contrôlant la synthèse d'AGI a permis la biosynthèse d'AGI par génie métabolique dans des graines de plantes cultivées. Nous présentons, ici quelques exemples de modifications de la composition en TAG chez le colza et tentons de comprendre quels peuvent être les facteurs limitant l'accumulation des AGI. Nous concluons que pour produire une huile homogène à un taux élevé, avec une nouvelle composition en acide gras chez une plante cultivée, il sera nécessaire de fournir un environnement optimal au transgène, de s'assurer que la plante est capable d'incorporer des AGI au niveau des TAG et enfin, de contrôler le métabolisme des lipides de stockage dans les graines en cours de développement chez ces plantes transgéniques.Mots-clés : acides gras inhabituels, triacylglycérols, ségrégation, Brassica napus.Summary : Vegetable-derived oils are of interest for industrial applications partly because of the chemical similarity of plant oils to mineral oils but also because of the economic need to reduce overproduction of seed oils for nutritional use. Complex oils can be produced in seeds as a low cost agricultural product based on renewable solar energy that requires less refining and is biodegradable and thus produces less adverse effects on the environment. In addition, biotechnologies have accelerated selection programmes and increased the genetic diversity available for the development of new varieties of oilseeds with specific fatty acid compositions. In the developing oilseed, energy and carbon are stored as lipid under the form of triacylglycerol, that is, a glycerol molecule to which three fatty acids are esterified. Fatty acids comprise a linear chain of carbon atoms, the first of which carries an organic acid group. The chain length and the presence of double bonds determine the properties of the fatty acid which in turn determine the physical and chemical properties of the oil of storage lipids and hence their economic value. In addition to the common C16-and C18-saturated and unsaturated fatty acids of membrane lipids, the seed storage lipids of many plant species contain unusual fatty acids (UFAs) which can vary in chain length, in the degree of unsaturation, possess double bonds in unusual positions, or can contain additional functional groups such as hydroxy, Article disponible sur le site http://www.ocl-journal.org ou http://dx

show abstract

Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases.

Cited by 283 publications

References 38 publications

Molecular cloning and characterisation of an acyl carrier protein thioesterase gene (CocoFatB1) expressed in the endosperm of coconut (Cocos nucifera) and its heterologous expression in Nicotiana tabacum to engineer the accumulation of different fatty acids

Molecular cloning and characterisation of an acyl carrier protein thioesterase gene (CocoFatB1) expressed in the endosperm of coconut (Cocos nucifera) and its heterologous expression in Nicotiana tabacum to engineer the accumulation of different fatty acids

ACX3, a Novel Medium-Chain Acyl-Coenzyme A Oxidase from Arabidopsis

Production of unusual fatty acids in rapeseed

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