Production of unusual fatty acids in rapeseed

Roscoe, Thomas; Maisonneuve, Sylvie; Delseny, Michel

doi:10.1051/ocl.2002.0024

Cited by 6 publications

(5 citation statements)

References 40 publications

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“…Rapeseed is an important potential source of edible unsaturated oils. It is one of the most oleaginous plants cultivated in the world and it offers several advantages in the application of biotechnical approaches to improve oil composition [9,10]. Sebei et al [11] reported that two Tunisian rapeseed varieties (Hybridol and Pactol) contained more than 60% of oleic acid, 18% of linoleic acid, and 8% of linolenic acid.…”

Section: Introductionmentioning

confidence: 99%

Evolution of phosphoenolpyruvate carboxylase activity and lipid content during seed maturation of two spring rapeseed cultivars (Brassica napus L.)

Sebei

Ouerghi

Kallel

et al. 2006

Comptes Rendus. Biologies

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Phosphoenolpyruvate carboxylase (PEPc: EC 4.1.1.31) activity was monitored during seed maturation of two varieties (Hybridol and Pactol) of rapeseed (Brassica napus L.), widely cultivated in Tunisia. In the Hybridol variety, PEPc activity did not exceed 5 µmol h −1 per gram of fresh weight (FW) during the first stages of maturation. It then highly increased to reach more than 30 µmol h −1 g −1 /FW. On the contrary, in the Pactol variety, the evolution of PEPc activity showed a classical curve, i.e. an increase during the most active phase of lipid accumulation in maturating seeds, followed by a rapid decrease until the end of seed maturation. In both varieties, the seed oil was characterised by a high content of oleic acid (C 18:1 ), linoleic (C 18:2 ) and linolenic acids (C 18:3 ). Saturated fatty acids were also present, although decreasing with maturation course. The analysis of the triacylglycerols (TAG) showed that trioleoylglycerol (OOO) and dioleoyllinoleoylglycerol (OOL) were the major species (ca. 35% and ca. 25% of the total respectively). The evolution pattern of fatty acids and TAG contents was similar to that of PEPc activity. Taken together, our findings suggest that PEPc may be involved in fatty acid and triacylglycerol biosynthesis during seed maturation of both rapeseed varieties.

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Section: Introductionmentioning

confidence: 99%

Evolution of phosphoenolpyruvate carboxylase activity and lipid content during seed maturation of two spring rapeseed cultivars (Brassica napus L.)

Sebei

Ouerghi

Kallel

et al. 2006

Comptes Rendus. Biologies

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show abstract

“…389). The development of transgenic plants to produce PUFA-and hydroxy-rich FFA and structured lipids has been reviewed (390)(391)(392)(393)(394)(395)(396)(397)(398)(399). As stated in a review by Jaworski and Cahoon (398) "the production of transgenic crop plants that efficiently synthesize and accumulate unusual FA is the next challenge" (p. 183).…”

Section: Other Biocatalytic Means Of Lipid Modificationmentioning

confidence: 97%

Enzyme‐Catalyzed modification of oilseed materials to produce eco‐friendly products

Hayes

2004

J Americ Oil Chem Soc

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Novel products produced from seed oil materials (TAG, phospholipids, and minor components such as tocopherols, sterols, stanols, and fatty acyl esters of the latter two) by enzyme-mediated purification or chemical modification are reviewed. The primary focus is on "value-added products" of current and potential use (particularly in the food, cosmetics, and pharmaceutical industries) that require the selectivity of enzymes and mild operating conditions, the latter being beneficial for polyunsaturated and oxygenated acyl groups. The paper briefly reviews the biochemistry of enzymes in lipid modification (lipases, phospholipases, and lipoxygenases) and discusses and assesses the current and future applications, current state of the art, and areas for future research for the following enzyme-mediated processes: isolation of polyunsaturated and oxygenated FFA; formation of structured TAG as nutraceuticals; formation of MAG, saccharide-FA esters, and other polyhydric alcohol ester as emulsifiers and surfactants; isolation and/or modification of tocopherols and sterols as antioxidants; formation of hydroperoxides as chemical intermediates; and modification of phospholipids for use in liposomes.Agriculturally derived feedstocks will play an increasingly important role in North America and worldwide as the cost of petroleum continues to increase and its availability decreases. Lipids (TAG, phospholipids, sterols, etc.) are an example of such a feedstock. Although lipids are readily modified by chemical means (reviewed in Refs. 1-5) and can be synthesized from petroleum-based feedstocks (6), there is an increased interest in modifying lipids via biocatalysis, particularly to manufacture lipid-based products in the food, cosmetics, and pharmaceutical industries, and for applications focused on environmental friendliness, due to the mild reaction conditions and narrow product distributions. In addition, biocatalysis has also played a role in isolating natural products, for instance, by temporarily modifying the molecule of interest to facilitate downstream separations. Reviews on biocatalytic modification of lipids have appeared during the last 15 yr (7-11) and more recently in two monographs (12,13). In addition, a recent conference entitled Enzymes in Lipid Modification was held in 2003 in Greifswald, Germany (14). The organization and emphasis of the previous reviews focused mostly on the types of biocatalysts; the emphasis here is on the products formed. Preceding the discussion of products is a brief overview of the important biocatalytic tools to be used. BIOCATALYSTS USED FOR LIPID MODIFICATIONLipases (EC 3.1.1.3). Lipases are specifically designed by nature for the hydrolysis and synthesis of ester bonds involving FA, and can form and/or hydrolyze amide, carbonate, and thioester bonds as well. Reactions catalyzed by lipases that involve the ester bond are diagrammed in Figure 1. Lipases are perhaps the most frequently used enzymes for biotransformations because of their ability to function in nonaqueous and aqueous ...

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“…These seed-specific isoforms optimise channelling of unusual fatty acids into TAG and a segregation away from membrane lipids [13] (see Fig. 1).…”

Section: The Role Of Acyltransferases In the Biosynthesis Of Seed Lipidsmentioning

confidence: 99%

Identification of acyltransferases controlling triacylglycerol biosynthesis in oilseeds using a genomics‐based approach

Roscoe

2005

Eur. J. Lipid Sci. Technol.

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Production of unusual fatty acids in rapeseed

Cited by 6 publications

References 40 publications

Evolution of phosphoenolpyruvate carboxylase activity and lipid content during seed maturation of two spring rapeseed cultivars (Brassica napus L.)

Evolution of phosphoenolpyruvate carboxylase activity and lipid content during seed maturation of two spring rapeseed cultivars (Brassica napus L.)

Enzyme‐Catalyzed modification of oilseed materials to produce eco‐friendly products

Identification of acyltransferases controlling triacylglycerol biosynthesis in oilseeds using a genomics‐based approach

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