PALMERSTON NORTH, NEW ZEALAND The effects of IVF aspiration on the temperature, dissolved oxygen levels, and pH of follicular fluid

Redding, Gabe P.; Bronlund, John E.; Hart, A. L.

doi:10.1007/s10815-005-9011-3

Cited by 22 publications

(15 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In accordance with these changes, we found higher pH values in the first set of samples compared to the second set of samples. Similar changes in the first part of the collected follicular fluid have been described by Redding et al (2006). However, the volume of the aspiration tubes and the concentration of CO 2 were not described and follicular fluid was obtained from slaughtered cows in this study.…”

Section: Discussionsupporting

confidence: 52%

“…Acid-base indicators can also be changed by storage temperature or type of syringe (plastic or glass) (Wu et al 1997;Beaulieu et al 1999). Because similar errors should be expected during and after collection of follicular fluid (Redding et al 2006), the requirements for anaerobic follicular fluid collection have been reported (Berg et al 2005;Redding et al 2006). However, changes in the quality of follicular fluid can occur even before aspiration due to increasing exposure to intraperitoneal CO 2 during artificial pneumoperitoneum with laparoscopical procedures for follicular aspiration.…”

Section: Discussionmentioning

confidence: 91%

“…We have found only one study that performed sampling of bovine follicular fluid from ovaries after slaughter (Redding et al 2006) and one study employing TVFA for this reason (Berg et al 2005). A few articles in human medicine have described the concentration of carbon dioxide and pH in follicular fluid (Daya 1988;Imoedemhe et al 1993) and oxygen concentration has been described in bovine follicular fluid after TVFA of preovulatory follicles (de Castro e Paula et al 2008).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle

et al. 2011

View full text Add to dashboard Cite

The aim of this study was to evaluate the applicability of ovum pick-up equipment for follicular fluid collection from various follicular structures (experiment 1) and for recovery of follicular fluid for acid-base balance analysis (experiment 2). An ultrasound scanner equipped with a 5-MHz convex transducer was used for transvaginal ultrasound-guided follicular aspiration. A 17-gauge, 60-cm aspiration needle was connected with a shortened aspiration line. The fluid was aspirated manually into a 2 ml plastic syringe at a speed of approximately 0.2 ml/s. The success of aspiration was higher in ovarian cysts (100%) and single follicles larger than 13 mm (76.7%) compared to single follicles smaller than 12 mm (20%, p < 0.001). The success of aspiration of multiple follicles on day 4 (diameter of 7-9 mm) was higher (90.9%) compared to follicles on day 2 (diameter of 4-6 mm) (66.7%, p < 0.05) in experiment 1. The fluid from ovarian cysts > 25 mm in diameter was aspirated in a two-step procedure (samples 1 and 2) for the determination of pH, HCO 3 , BE, pCO 2 and pO 2 (experiment 2). The indicators were compared between samples 1 and 2. Higher pO 2 as well as pH and lower pCO 2 in sample 1 compared to sample 2 showed insufficient anaerobic conditions during the first phase of the puncture in experiment 2. Our study brings for the first time the finding that the ovum pick-up equipment used in our experiments is suitable for the collection of follicular fluid only from larger follicular structures. The sampling of follicular fluid for acid-base balance assays requires the development of a special new device to prevent samples from coming into contact with air during aspiration.

show abstract

Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle

et al. 2011

View full text Add to dashboard Cite

show abstract

“…This is in agreement with studies performed with animal blood in which it has been reported that CO 2 can escape from the serum to the partial vacuum above by underfilling of the syringe or blood collection tubes (Bandi 1981;James et al 1997;Constable 2008). Therefore, we conclude that the ABB set allows for anaerobic sampling of the fluid from a reservoir that is required for follicular fluid collection as well (Berg et al 2005;Redding et al 2006). This set allows for the study of changes in pH, pO 2 and pCO 2 during the final part of follicular development similarly as in human follicular fluid (Fischer et al 1992), where a relationship between the developmental potential of the human oocyte and the dissolved oxygen content of follicular fluid has been proven (Van Blerkom et al 1997).…”

Section: Discussionmentioning

confidence: 86%

“…Nevertheless, for this analysis, there are many potential pre-analytical errors in blood sampling-e.g., caused by the underfilling of the syringe (Bandi 1981;James et al 1997), the syringe material itself or conditions of sample storage (Szenci and Besser 1990;Wu et al 1997;Beaulieu et al 1999;Knowles et al 2006;Cingi et al 2009). Because similar errors should be expected during and after the collection of follicular fluid (Redding et al 2006), requirements for anaerobic follicular fluid collection have been reported (Berg et al 2005;Redding et al 2006). There is evidence that changes in the quality of human follicular fluid can occur even before aspiration, due to increasing exposure to intraperitoneal CO 2 during artificial pneumoperitoneum with laparoscopical procedures for follicular aspiration.…”

mentioning

confidence: 93%

A new device for the aspiration of follicular fluid for acid-base balance analysis in cattle

Čech¹,

Doležel²,

Holickova³

et al. 2013

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:The aim of this study was to evaluate a new device for the ultrasound-guided transvaginal aspiration of follicular fluid for acid-base balance analysis (ABB set) in comparison with the original modified commercial OPU set. In the ABB set, an aspiration syringe was placed in the front part of the new tool's handle, next to the transducer, so as to enable direct collection of the sample into the syringe. To obtain a sufficient amount of testable fluid, reservoirs of urine (rubber balloons) were used for later aspiration under laboratory conditions in Experiment 1. Fifteen triads of samples (each triad with two punctures) were collected. While the first sample of each triad was taken using the ABB set (ABB sample), two samples were taken by one puncture using the original modified commercial OPU set: aerobic phase of sampling (AE sample) with air present in the tubing at the start of sampling and the subsequent anaerobic phase of sampling (AN sample). Values determined in the second sample from the triad (AE) varied from the values in both ABB and AN samples (pH 7.685 vs. 7.704 vs. 7.692, pCO 2 11.13 vs. 10.3 vs. 10.85, pO 2 6.87 vs. 8.67 vs. 7.02). In Experiment 2, ultrasound-guided transvaginal aspirations were carried out in 13 cows bearing ovarian cysts with diameters of at least 3 cm, using plastic aspiration syringes (Experiment 2P) and in 12 cows using glass aspiration syringes (Experiment 2G). The sequence of samples was the same as in Experiment 1. We found a significantly higher pH in AE in comparison to AN (7.357 vs. 7.348), lower pCO 2 (6.85) and higher O 2 (14.12) in samples of AE in comparison to samples of ABB and AN (pCO 2 7.36, 7.30; O 2 9.95, 10.63 respectively) in cystic fluid in Experiment 2P. We found a significantly higher pH (7.4), lower pCO 2 (5.98) and a higher pO 2 (12.35) in AE samples in comparison to ABB and AN samples of cystic fluid (pH 7.386, 7.385; pCO 2 6.39, 6.35 and O 2 10.56, 10.65, respectively) from Experiment 2G. We conclude that the acid-base balance assay was affected by air, present in the tubing during aerobic sampling in comparison to anaerobic and ABB set sampling. These pre-analytical changes can be prevented by the use of the ABB set because the results obtained with the ABB set were not different from that of the AN samples. We also confirmed pre-analytical changes in acid-base balance parameters in the cystic fluid after it had been stored in plastic aspiration syringes. Our new ABB set equipped with a glass aspiration syringe is suitable for sampling follicular fluid for both acid-base balance and gas analysis.Keywords: cow; acid-base parameters; ovarian cysts; cystic fluid collection; ovum pick-up List of abbreviations ABB = acid base balance, AE = aerobic, AN = anaerobic, OPU = ovum pick-up, TVFA = transvaginal follicular aspiration

show abstract

Epigenetic profile of developmentally important genes in bovine oocytes

Heinzmann

Hansmann

Herrmann

et al. 2011

Molecular Reproduction Devel

View full text Add to dashboard Cite

Assisted reproductive technologies are associated with an increased incidence of epigenetic aberrations, specifically in imprinted genes. Here, we used the bovine oocyte as a model to determine putative epigenetic mutations at three imprinted gene loci caused by the type of maturation, either in vitro maturation (IVM) in Tissue Culture Medium 199 (TCM) or modified synthetic oviduct fluid (mSOF) medium, or in vivo maturation. We applied a limiting dilution approach and direct bisulfite sequencing to analyze the methylation profiles of individual alleles (DNA molecules) for H19/IGF2, PEG3, and SNRPN, which are each associated with imprinting defects in humans and/or the mouse model, and are known to be differentially methylated in bovine embryos. Altogether, we obtained the methylation patterns of 203 alleles containing 4,512 CpG sites from immature oocytes, 213 alleles with 4,779 CpG sites from TCM-matured oocytes, 215 alleles/4,725 CpGs in mSOF-matured oocytes, and 78 alleles/1,672 CpGs from in vivo-matured oocytes. The total rate of individual CpGs and entire allele methylation errors did not differ significantly between the two IVM and the in vivo group, indicating that current IVM protocols have no or only marginal effects on these critical epigenetic marks. Furthermore, the mRNA expression profiles of the three imprinted genes and a panel of eight other genes indicative of oocyte competence were determined by quantitative real-time PCR. We found different mRNA expression profiles between in vivo-matured oocytes versus their in vitro-matured counterparts, suggesting an influence on regulatory mechanisms other than DNA methylation.

show abstract

PALMERSTON NORTH, NEW ZEALAND The effects of IVF aspiration on the temperature, dissolved oxygen levels, and pH of follicular fluid

Cited by 22 publications

References 14 publications

Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle

Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle

A new device for the aspiration of follicular fluid for acid-base balance analysis in cattle

Epigenetic profile of developmentally important genes in bovine oocytes

Contact Info

Product

Resources

About