Paleoproteomics explained to youngsters: how did the wedding of two-dimensional electrophoresis and protein sequencing spark proteomics on: Let there be light

Abstract

The significance of this manuscript is to recall and review the two decades that separated the first attempts of performing large scale analysis of proteins from the solid technical corpus that existed when the word "proteomics" was coined twenty years ago. This recollection is made within the scientific historical context of this decade, which also saw the blossoming of DNA cloning and sequencing. This article is part of a Special Issue entitled: 20 years of Proteomics in memory of Viatliano Pallini. Guest Editors: Luca Bini , Juan J. Calvete, Natacha Turck, Denis Hochstrasser and Jean-Charles Sanchez.

Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

“…At this point in time 1D-SDS-PAGE promised a glimpse at salivary gland protein complexity. However, our ability to identify these proteins with proteomics was not yet at a technological advanced level that would enable this (Rabilloud, 2020 ). The limitation of 1D-SDS-PAGE to reveal complexity was also limited by the resolution afforded and the fact that members of the same protein family would have very similar molecular weights, even if they have different iso-electric points resulting in underestimation of complexity.…”

A decade of Central and Eastern European Proteomic Conference (CEEPC): Credibility, cohesion and vision for the next decade

“…The commencement of an era of mass spectrometry approaches since the third milenium represented a quantum jump allowing quick and precise protein identification at peptide level, thus turning proteomics into an exciting biomedical research reality [2][3][4]. Currently, using modern instrumentation, we can explore up to five orders of magnitude in dynamic range of cell/tissue proteins, but some biological fluids including serum or cerebrospinal fluid encompass up to 10 -12 orders of magnitude [5] and yet calling for other novel or more powerful technologies and tools.…”

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

“…A decade after its introduction in the mid-1970s [ 1 , 2 ], two-dimensional electrophoresis was instrumental in the birth of proteomics [ 3 ] Since then, the exponential development of shotgun proteomics, pioneered at the turn of the century [ 4 ] and driven in great part by the gigantic increase in the sensitivity of mass spectrometers, has largely superseded two-dimensional (2D) gel-based proteomics. However, although often being referred to as an outdated method, 2D gel-based proteomics still has some merit, as recently reviewed [ 5 , 6 , 7 ].…”