Palaeoepidemiology of Schistosoma infection in mummies.

Miller, R. L.; Armelagos, George J.; Ikram, Salma; Jonge, Niels de; Krijger, F. W.; Deelder, André M.

doi:10.1136/bmj.304.6826.555

Cited by 60 publications

(21 citation statements)

References 5 publications

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“…The supernatant was then mixed with trichloroacetic acid solution (5%7%) and centrifuged at 25,000 g for 20 min. After dialysis of the supernatant for 24 h against distilled water (4C), extracted protein was used for ELISA (40). Immunochromatography has been applied to the detection of the plague bacterium Y. pestis specific F1 antigen in several burial sites in Europe (24,34,37,46,48).…”

Section: Analyzing Ancient Microbial Proteinsmentioning

confidence: 99%

Beyond ancient microbial DNA: Nonnucleotidic Biomolecules For Paleomicrobiology

et al. 2011

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Identifying the causes of past epidemics depends on the specific detection of pathogens in buried individuals; this field of research is known as paleomicrobiology, an emerging field that has benefited from technological advances in microbiology. For almost 15 years, the detection, identification, and characterization of microbes in ancient environmental and human specimens emerged on the basis of ancient DNA (aDNA) analyses. aDNA limitations due to potential contamination by modern DNA and altered aDNA led to the development of alternative methods for the detection and characterization of nonnucleotidic biomolecules, including mycolic acids (of ancient mycobacteria) and proteins. Accordingly, immunohistochemistry, immunochromatography, and enzyme-linked immunosorbent assay techniques have been developed for the specific detection of microbes from ancient human and environmental specimens. Protein analysis by mass spectrometry, a standard for ancient animal identification, has also recently emerged as a technique for ancient mycobacteria detection, while immuno-PCR is yet another promising technique. As with aDNA, strict protocols must be enforced to ensure authenticity of the data. Here we review the analysis of nonnucleotidic biomolecules from ancient microbes and the ability of these analyses to complement aDNA analyses, which opens new opportunities for identification of ancient microbes as well as new avenues to potentially resolve controversies regarding the cause of some historical pandemics and study the coevolution of microbes and hosts.

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Section: Analyzing Ancient Microbial Proteinsmentioning

confidence: 99%

Beyond ancient microbial DNA: Nonnucleotidic Biomolecules For Paleomicrobiology

et al. 2011

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“…Sambon proposed the new species as Schistosoma mansoni in 1907, and it was fully described by Pirajá-da-Silva a year later, in Salvador, Brazil. Interestingly, in 1910, Rufer found calcified eggs in the kidneys of Egyptian mummies of the 20th Dynasty (1250 to 1000 BCE), but there are even more ancient (3000 BCE) molecular indications of human infection (188).…”

Section: Historymentioning

confidence: 99%

Update on Eosinophilic Meningoencephalitis and Its Clinical Relevance

2009

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SUMMARY Eosinophilic meningoencephalitis is caused by a variety of helminthic infections. These worm-specific infections are named after the causative worm genera, the most common being angiostrongyliasis, gnathostomiasis, toxocariasis, cysticercosis, schistosomiasis, baylisascariasis, and paragonimiasis. Worm parasites enter an organism through ingestion of contaminated water or an intermediate host and can eventually affect the central nervous system (CNS). These infections are potentially serious events leading to sequelae or death, and diagnosis depends on currently limited molecular methods. Identification of parasites in fluids and tissues is rarely possible, while images and clinical examinations do not lead to a definitive diagnosis. Treatment usually requires the concomitant administration of corticoids and anthelminthic drugs, yet new compounds and their extensive and detailed clinical evaluation are much needed. Eosinophilia in fluids may be detected in other infectious and noninfectious conditions, such as neoplastic disease, drug use, and prosthesis reactions. Thus, distinctive identification of eosinophils in fluids is a necessary component in the etiologic diagnosis of CNS infections.

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“…Les résultats de cette étude, s'ajoutent à ceux de travaux antérieurs réalisés pour détecter des unicellulaires sanguins, Plasmodium falciparum (Miller et al, 1994 ;Cerruti et al, 1999), Trypanosoma cruzi (Fornaciari et al, 1992) et des helminthes, Schistosoma sp. (Deelder et al, 1990 ;Miller et al, 1992). Ils prouvent la possibilité d'utiliser l'outil immunologique sur le matériel archéologique, et mettent en exergue le caractère incontournable de ces techniques de pointe.…”

Section: Discussionunclassified

Paléoparasitologie et immunologie. L’exemple d’Entamoeba histolytica

Bailly¹,

Bouchet²

2006

archeosciences

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Palaeoepidemiology of Schistosoma infection in mummies.

Cited by 60 publications

References 5 publications

Beyond ancient microbial DNA: Nonnucleotidic Biomolecules For Paleomicrobiology

Beyond ancient microbial DNA: Nonnucleotidic Biomolecules For Paleomicrobiology

Update on Eosinophilic Meningoencephalitis and Its Clinical Relevance

Paléoparasitologie et immunologie. L’exemple d’Entamoeba histolytica

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