Pairing <i>Bacteroides vulgatus</i> LPS Structure with Its Immunomodulatory Effects on Human Cellular Models

Lorenzo, Flaviana Di; Pither, Molly Dorothy; Martufi, Michela; Scarinci, Ilaria; Guzmán-Caldentey, Joan; Łakomiec, Ewelina; Jachymek, Wojciech; Bruijns, Sven C. M.; Santamaría, Sonsoles Martín; Frick, Julia-Stefanie; Kooyk, Yvette van; Chiodo, Fabrizio; Silipo, Alba; Bernardini, Maria Lina; Molinaro, Antonio

doi:10.1021/acscentsci.0c00791

Cited by 66 publications

(78 citation statements)

References 57 publications

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“…ET-B showed high fatty acid metabolism and LPS production ( Figure 4 ), a common endotoxin in the human body. LPS produced by intestinal bacteria can trigger systemic and local pro-inflammatory and immunomodulatory responses [ 55 ]. In the research of the intestinal type of diabetic patients, it also has concluded that ET-B has more LPS and TNF-α content [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

A Korean-Style Balanced Diet Has a Potential Connection with Ruminococcaceae Enterotype and Reduction of Metabolic Syndrome Incidence in Korean Adults

Unno

Kang

et al. 2021

Nutrients

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Metabolic syndrome is associated with usual dietary patterns that may be involved in enterotypes. We aimed to understand the potential relationship of enterotypes and dietary patterns to influence metabolic syndrome in the Koreans. Using the Korea National Health and Nutrition Examination Survey (KNHANES)-VI in 2014, metabolic parameters were also analyzed among the dietary patterns classified by principal component analysis in Korean adults. The fecal microbiota data of 1199 Korean adults collected in 2014 were obtained from the Korea Centers for Disease Control and Prevention. Enterotypes were classified based on Dirichlet multinomial mixtures (DMM) by Mothur v.1.36. The functional abundance of fecal bacteria was analyzed using the PICRUSt2 pipeline. Korean adults were clustered into three dietary patterns including Korean-style balanced diets (KBD, 20.4%), rice-based diets (RBD, 17.2%), and Western-style diets (WSD, 62.4%) in KNHANES. The incidence of metabolic syndrome was lowered in the order of RBD, WSD, and KBD. The participants having a KBD had lower serum C-reactive protein and triglyceride concentrations than those with RBD and WSD (p < 0.05). Three types of fecal bacteria were classified as Ruminococcaceae type (ET-R, 28.7%), Prevotella type (ET-P, 52.2%), and Bacteroides type (ET-B, 42.1%; p < 0.05). ET-P had a higher abundance of Prevotella copri, while ET-R contained a higher abundance of Alistipes, Akkermansia muciniphila, Bifidobacterium adolescentis, and Faecalibacterium prausnitzii. ET-B had a higher abundance of the order Bilophila (p < 0.05). Metabolism of propanoate, starch, and sucrose in fecal microbiome was higher in ET-P and ET-R, whereas fatty acid metabolism was enhanced in ET-B. Fecal microbiota in ET-P and ET-B had higher lipopolysaccharide biosynthesis activity than that in ET-R. The metabolic results of KBD and RBD were consistent with ET-R and ET-P’s gut microbiota metabolism, respectively. In conclusion, Korean enterotypes of ET-P, ET-B, and ET-R were associated with RBD, WSD, and KBD, respectively. This study suggests a potential link between dietary patterns, metabolic syndrome, and enterotypes among Korean adults.

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Section: Discussionmentioning

confidence: 99%

A Korean-Style Balanced Diet Has a Potential Connection with Ruminococcaceae Enterotype and Reduction of Metabolic Syndrome Incidence in Korean Adults

Unno

Kang

et al. 2021

Nutrients

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“…Also, the administration of Bacteroides LPS re-established intestinal immune homeostasis in a mouse model for experimental colitis, thus correlating the health-promoting effects to the weak agoniztic properties of this LPS 24,25 . Further, in the human innate immune system, we demonstrated a relevant capability of the LPS to induce antiinflammatory cytokines 26 . The O-antigen of B. vulgatus mpk LPS consists of a disaccharide repeating unit of 27 (Fig.…”

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confidence: 84%

Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus

Zhu

Shen

Chiodo³

et al. 2020

Nat Commun

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Glycans are involved in various life processes and represent critical targets of biomedical developments. Nevertheless, the accessibility to long glycans with precise structures remains challenging. Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota. The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction has enabled us to synthesize up to a 128-mer glycan. The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.

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“…1 × 10 6 BMDCs/ml were stimulated with PBS (mock, Thermo Fisher Scientific), B. vulgatus or E. coli at a multiplicity of infection (MOI) of 1 at 37°C. 100 ng/ml isolated LPS of B. vulgatus [LPS BV , isolated as described in ( 26 , 27 ) and ( 28 )] or E. coli [LPS EC , isolated as described in ( 26 , 27 ) and ( 28 )] were used for stimulation. For stimulation with a complex microbiota, fecal samples were collected from SPF Rag1 -/- mice prior to administration of bacteria and induction of colitis, as well as at the end of the experiment (see T cell transfer in Rag1 -/- mice).…”

Section: Methodsmentioning

confidence: 99%

Gut Commensal-Induced IκBζ Expression in Dendritic Cells Influences the Th17 Response

et al. 2021

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Intestinal commensal bacteria can have a large impact on the state of health and disease of the host. Regulation of Th17 cell development by gut commensals is known to contribute to their dichotomous role in promoting gut homeostasis and host defense, or development of autoimmune diseases. Yet, the underlying mechanisms remain to be fully elucidated. One candidate factor contributing to Th17 differentiation, and the expression of which could be influenced by commensals is the atypical nuclear IκB protein IκBζ. IκBζ acts as a transcriptional regulator of the expression of Th17-related secondary response genes in many cell types including dendritic cells (DCs). Insights into the regulation of IκBζ in DCs could shed light on how these immune sentinel cells at the interface between commensals, innate and adaptive immune system drive an immune-tolerogenic or inflammatory Th17 cell response. In this study, the influence of two gut commensals of low (Bacteroides vulgatus) or high (Escherichia coli) immunogenicity on IκBζ expression in DCs and its downstream effects was analyzed. We observed that the amount of IκBζ expression and secretion of Th17-inducing cytokines correlated with the immunogenicity of these commensals. However, under immune-balanced conditions, E. coli also strongly induced an IκBζ-dependent secretion of anti-inflammatory IL-10, facilitating a counter-regulative Treg response as assessed in in vitro CD4+ T cell polarization assays. Yet, in an in vivo mouse model of T cell-induced colitis, prone to inflammatory and autoimmune conditions, administration of E. coli promoted an expansion of rather pro-inflammatory T helper cell subsets whereas administration of B. vulgatus resulted in the induction of protective T helper cell subsets. These findings might contribute to the development of new therapeutic strategies for the treatment of autoimmune diseases using commensals or commensal-derived components.

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Pairing Bacteroides vulgatus LPS Structure with Its Immunomodulatory Effects on Human Cellular Models

Cited by 66 publications

References 57 publications

A Korean-Style Balanced Diet Has a Potential Connection with Ruminococcaceae Enterotype and Reduction of Metabolic Syndrome Incidence in Korean Adults

A Korean-Style Balanced Diet Has a Potential Connection with Ruminococcaceae Enterotype and Reduction of Metabolic Syndrome Incidence in Korean Adults

Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus

Gut Commensal-Induced IκBζ Expression in Dendritic Cells Influences the Th17 Response

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