PAHs biodegradation potential of indigenous consortia from agricultural soil and contaminated soil in two-liquid-phase bioreactor (TLPB)

Wang, Congying; Wang, Fang; Wang, Tao; Bian, Yongrong; Yang, Xinglun; Jiang, Xin

doi:10.1016/j.jhazmat.2009.10.123

Cited by 64 publications

(23 citation statements)

References 55 publications

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“…This result is in accordance with LiPs isolated from Phanerochaete sordida YK-624, which was purified to homogeneity by anion-exchange and gel permeation chromatography (Wang et al, 2010).The molecular mass of LiP isoenzymes obtained in the current study was different from that of white-rot fungal LiP, according to Fakoussa & Hofrichter (1999); the subunit molecular mass range of WRF was 38-47 kDa and that of peroxidases was 38-50 kDa. These results agree to some extent with those reported by other researchers (Yadav et al, 2010).…”

Section: Characterization Of the Isoenzymes Estimation Of Molecular Msupporting

confidence: 87%

Purification and Characterization of Lignin Peroxidase Isozymes from Humicola grisea (Traaen) and Its Application in Bioremediation of Textile Dyes

Moubasher¹,

Mostafa²,

Wahsh³

et al. 2017

Egypt. J. Bot.

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PEROXIDASES have numerous important applications in industries and biodegradation of organopollutants , so there is a need to explore more and more sources of enzymes with different characteristics. In the present study, lignin peroxidases (LiPs) producing by fungus Humicola grisea were extracted and purified using gel filtration and ion exchange chromatography. Protein and heme protein were eluted in different peaks about 6 of these peaks had LiP activity and this is an indication that LiP in H. grisea was isoenzymes. Four of these isoenzymes were characterized regarding their molecular mass, optimum temperature and optimum pH for activity, substrate concentration and effect of some metal ions. The four purified isoenzymes have maximum activities at different temperatures, where optimum temperature for H2 and H4 isoenzyme were at 60 ºC, and the optimum temperatures for H1 and H3 were at 40 ºC and 30 ºC, respectively. The maximum activities of the four isoenzymes were within the acidic range. The different LiP isoenzymes showed different Km and Vmax values representing the varied affinity of the four isoenzymes using veratryl alcohol as a substrate. Km values for H1, H2, H3 and H4 were 5.26, 5.0, 1.3 and 1.4, respectively. While Vmax values for the same isoenzymes were 1.25, 0.8, 0.87 and 1.1, respectively.

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Section: Characterization Of the Isoenzymes Estimation Of Molecular Msupporting

confidence: 87%

Purification and Characterization of Lignin Peroxidase Isozymes from Humicola grisea (Traaen) and Its Application in Bioremediation of Textile Dyes

Moubasher¹,

Mostafa²,

Wahsh³

et al. 2017

Egypt. J. Bot.

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“…The 24 hour equilibrating time was confirmed by Birch 152 et al (2010), and the biological half-time for the chosen PAHs were in the range of 2 to 2200 days 153 (Wang et al, 2010) assuring that no biological degradation will occur during the experiments. For all 154 experiments an identical filtration and extraction procedure was used following the flow-diagram in 155 the filtrated sample (3.5 L), 1 L was taken out for LLE and 1 L aliquots for SPE C 18 -disc; to each of 160 these, pyrene-d 10 and perylene-d 12 were added as internal standards.…”

Section: Extraction Of Pahs 150mentioning

confidence: 56%

Particle phase distribution of polycyclic aromatic hydrocarbons in stormwater — Using humic acid and iron nano-sized colloids as test particles

Nielsen

Kalmykova

Strömvall

et al. 2015

Science of The Total Environment

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“…The simulated bioremediation of fluoranthene in soil-water system (Fig. 5) manifested the colonizing and degradation capacity of H. chlorophenolicum FA1 along with competition with indigenous flora, which was comparable to some other bacterial isolates (TrzesickaMlynarz and Ward 1996;Tam and Wong 2008;Wang et al 2010). Therefore, the isolated strain FA1 could be applied for further exploration.…”

Section: Discussionmentioning

confidence: 74%

Degradation of fluoranthene by a newly isolated strain of Herbaspirillum chlorophenolicum from activated sludge

Qiu

et al. 2010

Biodegradation

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A fluoranthene-degrading bacterial strain FA1 was isolated from activated sludge and identified as Herbaspirillum chlorophenolicum, a newfound bacterial species that can grow well on fluoranthene as sole carbon and energy source. The kinetic characteristic of strain FA1 was tested in the aqueous model system (AMS) and the effects of nonionic surfactants on fluoranthene biodegradation in the AMS were then investigated. Tween 80 exhibited the best solubilization capacity for fluoranthene among three surfactants and its bioavailability decreased with an increase in its concentration and its degradation kinetics fit well with the first-order of power index model. The biotransformation of fluoranthene was greatly improved by Tween 80, and 58.5% fluoranthene degradation was obtained as Tween 80 was 100 mg/l. However, the bioavailability of fluoranthene decreased gradually with the increase of Tween 80 concentration. Bioremediation tests for fluoranthene in soil-water system were designed further to examine the degrading ability of strain FA1 with the presence of indigenous flora or not. The measurements showed that in the presence of indigenous flora, the optimum 30-day fluoranthene degradation in soil-water system reached 77.4%. Evidently, strain FA1 seems both efficient and high-effective and deserves further exploration on the enhanced bioremediation technologies for the treatment of fluoranthene-polluted soil.

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PAHs biodegradation potential of indigenous consortia from agricultural soil and contaminated soil in two-liquid-phase bioreactor (TLPB)

Cited by 64 publications

References 55 publications

Purification and Characterization of Lignin Peroxidase Isozymes from Humicola grisea (Traaen) and Its Application in Bioremediation of Textile Dyes

Purification and Characterization of Lignin Peroxidase Isozymes from Humicola grisea (Traaen) and Its Application in Bioremediation of Textile Dyes

Particle phase distribution of polycyclic aromatic hydrocarbons in stormwater — Using humic acid and iron nano-sized colloids as test particles

Degradation of fluoranthene by a newly isolated strain of Herbaspirillum chlorophenolicum from activated sludge

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