Paeonol and Paeoniflorin, the Main Active Principles of Paeonia albiflora, Protect the Heart from Myocardial Ischemia/Reperfusion Injury in Rats

Abstract

The aim of this study was to investigate the effects of paeoniflorin (PF) and paeonol (PN), the main active compounds of the Paeonia albiflora Pallas, on myocardial ischemia and reperfusion (I/R)-induced injury in Sprague-Dawley rats IN VIVO. Under anesthesia, the rats were subjected to 25 min of ischemia by ligation of the left anterior descending coronary artery (LAD) followed by 6 h (Western blot analysis) or 24 h (hemodynamics and infarct size) of reperfusion. When the infarct size was measured as the percentage of the area at risk, both PF (25.0 % +/- 7.0 %) and PN (24.1 % +/- 5.5 %) significantly (P < 0.05) reduced it compared to I/R control (54.8 % +/- 2.6 %). Administration of 10 mg/kg PF or PN 1 h prior to I/R injury also resulted in a significant improvement of the hemodynamic parameters. Furthermore, both PF and PN decreased the caspase-3 and Bax expressions but up-regulated Bcl-2 in the left ventricles. The results show that both PF and PN reduced myocardial damage in rat through protection from apoptosis, suggesting that Paeonia albiflora Pallas might be useful in treating myocardial infarction.

Neuroprotective Capabilities of Tanshinone IIA against Cerebral Ischemia/Reperfusion Injury via Anti-apoptotic Pathway in Rats

“…[27][28][29] Previous studies have demonstrated that TSA can protect the brain from I/R injury. 30,31) However, whether TSA has a neuroprotective effect against apoptosis remains unknown.…”

Cardioprotection by Guanxin II in rats with acute myocardial infarction is related to its three compounds

“…Tanshinol possess the protection for myocardial mitochondrial membranes and microcirculatory disturbance from ischemia-reperfusion injury (Zhao et al, 1996;Han et al, 2008). And Nizamutdinova et al (2008) had also reported that paeoniflorin could protect the heart from myocardial ischemia/reperfusion injury when high-dosage paeoniflorin was given by peritoneal injection in rats. In the present study, FAT had slight or moderate effect on infarct size and PE had no effect, when given alone by gavage to AMI in the dosage of GXII (Fig.…”

“…The content of these water-soluble FATPE components in GXII is relatively high. FATE are all effective against myocardial ischemia (Huang et al, 1996;Zhao et al, 1996;Liu et al, 2006;Han et al, 2008;Nizamutdinova et al, 2008). FATPE of GXII were used to test the ABC hypothesis via bioethnopharmaceutical analytical pharmacology (BAP) (Huang, 2002a).…”

Paeoniflorin attenuates hippocampal damage in a rat model of vascular dementia

“…1) is the principal bioactive component of Paeoniae radix which has been used for >1,000 years in traditional Chinese medicine (11). In modern pharmacological studies, PF exhibits numerous pharmacological effects such as anti-inf lammation, antioxidant, endothelium-dependent vasorelaxation, neuromuscular blocking and cognition enhancement properties (12,13).…”