Paeoniflorin inhibits proliferation and migration of psoriatic keratinocytes via the lncRNA NEAT1/miR-3194-5p/Galectin-7 axis

Abstract

To investigate the mechanism underlying the effect of paeoniflorin (PF) on the proliferation and migration of psoriatic keratinocytes. The expressions of long noncoding RNA NEAT1, miR-3194-5p and Galectin-7 in skin tissues from psoriatic patients and healthy controls were detected. Psoriatic HaCat cells were used to investigate the function of NEAT1 and Galectin-7 as well as the effect and mechanism of PF in psoriasis. MTT, colony formation and scratch assays were used to assess the proliferation and migration of psoriatic HaCat cells. Dual-luciferase reporter assay was used to validate the interactions among NEAT1, miR-3194-5p and Galectin-7. NEAT1 and Galectin-7 were lowly expressed and miR-3194-5p was highly expressed in psoriatic patients. PF suppressed the proliferation and migration of psoriatic HaCat cells by elevating the expressions of NEAT1 and Galectin-7. NEAT1 positively mediated the expression of Galectin-7 by targeting miR-3194-5p. PF controls the proliferation and migration of psoriatic HaCat cells via the NEAT1/miR-3194-5p/Galectin-7 axis. Anti-Cancer Drugs 33: e423-e433

Paeoniflorin inhibits colorectal cancer cell stemness through the miR‐3194‐5p/catenin beta‐interacting protein 1 axis

“…To reflect the heterogeneity of miR‐3194‐5p in CRC cell lines, it is necessary to further detect the effects of miR‐3194‐5p in more types of CRC cells in the future. A previous study demonstrated that PF suppressed psoriatic keratinocyte proliferation and migration by reducing miR‐3194‐5p expression 14 . However, the mechanism and role of miR‐3194‐5p in PF‐mediated anticancer activity for CRC have not yet been elucidated.…”

“…Collectively, PF inhibited CRC cell stemness by repressing the miR‐3194‐5p/CTNNBIP1 axis to inhibit Wnt/β‐catenin signaling activation. Wang et al showed that PF could inhibit the proliferation and migration of psoriatic keratinocytes through the lncRNA NEAT1/miR‐3194‐5p/galectin‐7 axis 14 . Notably, galectin‐7 plays an anticancer role in CRC 35 .…”

“…A previous study demonstrated that PF suppressed psoriatic keratinocyte proliferation and migration by reducing miR-3194-5p expression. 14 However, the mechanism and role of miR-3194-5p in PF-mediated anticancer activity for CRC have not yet been elucidated. Our results demonstrated that miR-3194-5p overexpression partly abolished the repression of CRC cell malignant behaviors by PF.…”

“…Wang et al showed that PF could inhibit the proliferation and migration of psoriatic keratinocytes through the lncRNA NEAT1/miR-3194-5p/galectin-7 axis. 14 Notably, galectin-7 plays an anticancer role in CRC. 35 However, whether miR-3194-5p promotes CRC progression by targeting galectin-7 is worth further study.…”

“…Notably, previous research has reported that PF inhibited the proliferation and migration of psoriasis cells by downregulating miR-3194-5p. 14 Thus, considering the anti-CRC effect of PF and the inhibitory effect of PF on miR-3194-5p, we reasonably hypothesized that PF inhibits CRC progression by acting on miR-3194-5p.…”