Paeoniflorin inhibits IL‑1β‑induced expression of inflammatory mediators in human osteoarthritic chondrocyte

Zhao, Lin; Chang, Qi; Huang, Tao; Huang, Chang-lin

doi:10.3892/mmr.2017.8222

Cited by 11 publications

(16 citation statements)

References 28 publications

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“…In human pancreatic cancer cells, paeoniflorin has potential anti-tumor functions, enhancing apoptosis via the inhibition of MMP-9 and extracellular-related kinase signaling (26). A recent study by Zhao et al (27) reported that paeoniflorin is able to reduce the IL-1β-induced upregulation of inflammatory mediators and MMPs in human chondrocytes, which is similar to the results of the present study.…”

Section: Discussionsupporting

confidence: 91%

Paeoniflorin inhibits IL‑1β‑induced MMP secretion via the NF‑κB pathway in chondrocytes

Sun

Jiang

et al. 2018

Exp Ther Med

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Paeoniflorin serves important cellular roles, exerting anti-cancer, anti-inflammatory and anti-pulmonary fibrosis effects and possesses immune-modulatory properties. However, the exact role of paeoniflorin in the pathogenesis of osteoarthritis (OA) remains unclear. The aim of the present study was to investigate the effects of paeoniflorin on articular surfaces . Rat chondrocytes were cultured and an MTT assay was performed to assess chondrocyte survival. Following treatment with interleukin (IL)-1β and paeoniflorin, the production of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases-1 (TIMP-1) was examined using reverse transcription-quantitative polymerase chain reaction and western blotting. The interleukin (IL)-1β-induced nuclear factor (NF)-κB pathway activation was also investigated. The results demonstrated that paeoniflorin was able to downregulate the expression of MMP and increase the expression of TIMP-1ntmRNA and protein in IL-1β-induced rat chondrocytes. Furthermore, treating chondrocytes with paeoniflorin blocked the activation of NF-κB. These results suggest that paeoniflorin may serve am anti-catabolic role in the progression of OA and may be an effective preventative treatment for OA.

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Section: Discussionsupporting

confidence: 91%

Paeoniflorin inhibits IL‑1β‑induced MMP secretion via the NF‑κB pathway in chondrocytes

Sun

Jiang

et al. 2018

Exp Ther Med

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“…Pae is a monoterpene glycoside compounds with immunosuppressive, anti-inflammatory and antiproliferation activities. It is the main active ingredient of traditional Chinese medicine peony (16)(17)(18). Recent studies have found that Pae can inhibit the secretion of HIF-1α through mTOR signaling pathway, reduce the expression of α-SMA and collagen protein and relieve liver fibrosis (19).…”

Section: Discussionmentioning

confidence: 99%

Effect of paeoniflorin on cardiac remodeling in chronic heart failure rats through the transforming growth factor β1/Smad signaling pathway

Liu¹,

Ai²,

Feng³

et al. 2019

Cardiovasc. Diagn. Ther

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Background: Cardiac remodeling is an important mechanism for the occurrence and development of chronic heart failure (CHF). Paeoniflorin (Pae) is the main active ingredient of Chinese herbaceous peony and has novel anti-inflammatory effect. This study was conducted to assess the effects and mechanisms of Pae on cardiac remodeling in CHF rats. Methods: A cardiac remodeling rat model was induced by isoprenaline (Iso). Pae (20 μg/kg/d) was administrated to CHF rats for six weeks. Cardiac ultrasound was used to assess the structure and function of CHF rats. Collagen volume fraction (CVF) and perivascular collagen volume area of myocardial tissues were calculated. With real-time polymerase chain reaction and Western blot, the protein and mRNA levels of transforming growth factor β1 (TGF-β1) and Smad3 were detected. Results: Compared to Iso group, Pae can alleviate cardiac remodeling and improve cardiac function in CHF rats. The levels of CVF and perivascular collagen volume area reduced in Pae group (P<0.05). The expression of TGF-β1 and Smad3 protein decreased in Pae and Cap group (P<0.05). Further, the expression of TGF-β1 and Smad3 mRNA also decreased markedly in the Pae group (P<0.05). Conclusions: Pae could attenuate cardiac remodeling and improve cardiac function in CHF rats. The potential mechanism for the cardioprotective effect of Pae may be highly associated with the downregulating of TGF-β1/Smad signaling pathway.

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“…27,28 What's more, paeoniflorin has been reported as protective effects against OAwith anti-apoptosis and anti-inflammation effects on chondrocyte. 29,30 As for other key active ingredients, formononetin existing in HL and RG has been proved the anti-catabolic effects on rat chondrocytes and articular cartilage, 31 and it dose-dependently decreased IL-6 in OA subchondral osteoblasts. 32 Stigmasterol existing in HL and RAS, was demonstrated to block cartilage degradation in the rabbit model of OA, showing potential anti-osteoarthritic properties.…”

Section: Discussionmentioning

confidence: 99%

“…45,46 Encouragingly, anti-inflammatory effects have been demonstrated by many herbs of SHM in treating OA. For example, reducing serum levels of inflammatory mediators such as IL-6 in the rat OA model was the result of the action of HL, RPA and RD, 29,30,47,48 showing a therapeutic agent for the treatment of human OA. Especially, acute inflammation and chronic inflammatory conditions in the collagen-induced arthritis model would also benefit from RG therapy.…”

Section: The Anti-inflammatory Effect Was the Pharmacological Mechanimentioning

confidence: 99%

“…45 Interestingly, the IL-6 level reduced by HL, RPA and RD of SHM has been well examined in the rat OA model. 29,30,47,48 Of important, Miller also wondered whether TLR-4 could provide a direct link between tissue damage and pain sensitization. 52 Recently anti-acute and chronic inflammatory potential of RG in SHM was considered to involve in the TLR-4 pathway.…”

Section: Analgesic Effects Of Shm Against Oa May Be Related To Its Anmentioning

confidence: 99%

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<p>Validation of the Key Active Ingredients and Anti-Inflammatory and Analgesic Effects of Shenjin Huoxue Mixture Against Osteoarthritis by Integrating Network Pharmacology Approach and Thin-Layer Chromatography Analysis</p>

Yu¹,

Ma²,

Song³

et al. 2020

DDDT

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Background: Shenjin Huoxue Mixture (SHM), a classic traditional herb mixture has shown significant clinical efficacy against osteoarthritis (OA). Our previous experimental study has confirmed its anti-inflammatory and analgesic effect on acute soft tissue injury in rats, with the compound of glycyrrhizinate in SHM identified and the content of paeoniflorin in SHM determined by high-performance liquid chromatography (HPLC). However, the components and its pharmacological mechanisms of SHM against OA have not been systematically elucidated yet. Thus this study aimed to predict the key active ingredients and potential pharmacological mechanisms of SHM in the treatment of OA by network pharmacology approach and thin-layer chromatography (TLC) validation. Methods: The active ingredients of SHM and their targets, as well as OA-related targets, were identified from databases. The key active ingredients were defined and ranked by the number of articles retrieved in PubMed using the keyword "(the active ingredients [Title/Abstract]) AND Osteoarthritis[Title/Abstract] ", and validated partially by TLC. The pharmacological mechanisms of SHM against OA were displayed by GO term and Reactome pathway enrichment analysis with Discovery Studio 3.0 software docking to testing the reliability. Results: Finally, 16 key active ingredients were identified and ranked, including quercetin validated through TLC. Inflammatory response, IL-6 signaling pathway and toll-like receptor (TLR) cascades pathway were predicted as the main pharmacological mechanisms of SHM against OA. Especially, 12 out of 16 key active ingredients, including validated quercetin, were well docked to IL-6 proteins. Conclusion: Our results confirmed the anti-inflammatory and analgesic effect of SHM against OA through multiple components, multiple targets and multiple pathways, which revealed the theoretical basis of SHM against OA and may provide a new drug option for treating OA.

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Paeoniflorin inhibits IL‑1β‑induced expression of inflammatory mediators in human osteoarthritic chondrocyte

Cited by 11 publications

References 28 publications

Paeoniflorin inhibits IL‑1β‑induced MMP secretion via the NF‑κB pathway in chondrocytes

Paeoniflorin inhibits IL‑1β‑induced MMP secretion via the NF‑κB pathway in chondrocytes

Effect of paeoniflorin on cardiac remodeling in chronic heart failure rats through the transforming growth factor β1/Smad signaling pathway

<p>Validation of the Key Active Ingredients and Anti-Inflammatory and Analgesic Effects of Shenjin Huoxue Mixture Against Osteoarthritis by Integrating Network Pharmacology Approach and Thin-Layer Chromatography Analysis</p>

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