Paeoniflorin inhibits IL‑1β‑induced MMP secretion via the NF‑κB pathway in chondrocytes

Abstract

Paeoniflorin serves important cellular roles, exerting anti-cancer, anti-inflammatory and anti-pulmonary fibrosis effects and possesses immune-modulatory properties. However, the exact role of paeoniflorin in the pathogenesis of osteoarthritis (OA) remains unclear. The aim of the present study was to investigate the effects of paeoniflorin on articular surfaces . Rat chondrocytes were cultured and an MTT assay was performed to assess chondrocyte survival. Following treatment with interleukin (IL)-1β and paeoniflorin, the production of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases-1 (TIMP-1) was examined using reverse transcription-quantitative polymerase chain reaction and western blotting. The interleukin (IL)-1β-induced nuclear factor (NF)-κB pathway activation was also investigated. The results demonstrated that paeoniflorin was able to downregulate the expression of MMP and increase the expression of TIMP-1ntmRNA and protein in IL-1β-induced rat chondrocytes. Furthermore, treating chondrocytes with paeoniflorin blocked the activation of NF-κB. These results suggest that paeoniflorin may serve am anti-catabolic role in the progression of OA and may be an effective preventative treatment for OA.

Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-κB/NLRP3-mediated epithelial–mesenchymal transition and inflammation

“…It was well known that NF-κB used to serving as the upstream of NLRP3 53 . However, overexpression of NLRP3 can also lead to the increase of IL-1β and IL-18, and many literatures reported that IL-1β feedback loop could promote the activation of NF-κB signaling pathway [54][55][56][57][58][59] . Therefore, in our results, overexpression of NLRP3 caused the activation of NF-κB signaling pathway, although NF-κB served as the upstream of NLRP3.…”

Catalpol Attenuates IL-1β Induced Matrix Catabolism, Apoptosis and Inflammation in Rat Chondrocytes and Inhibits Cartilage Degeneration

“…IL-1β is widely applied in studies of the pathophysiology of OA, to trigger the release of inflammatory cytokines as well as chondrocyte apoptosis [20]. Regulation of the levels of several catabolic enzymes (MMPs or ADAMTS) is achieved by transcription factors, especially nuclear factor-kappa B (NF-κB) [21,22].…”

Therapeutic Single Compounds for Osteoarthritis Treatment

“…Particularly, some studies reported that paeoniflorin suppresses OA. IL-1β-induced MMP1, MMP3, and MMP13 expression was reduced and issue inhibitor of metalloproteinase-1 expression was increased in chondrocytes pretreated with paeoniflorin [ 84 , 85 ]. Furthermore, paeoniflorin suppressed NF-κB signaling, as indicated by increased inhibitor of NF-κB (IκB) and decreased p65 protein levels [ 84 , 85 ].…”

“…IL-1β-induced MMP1, MMP3, and MMP13 expression was reduced and issue inhibitor of metalloproteinase-1 expression was increased in chondrocytes pretreated with paeoniflorin [ 84 , 85 ]. Furthermore, paeoniflorin suppressed NF-κB signaling, as indicated by increased inhibitor of NF-κB (IκB) and decreased p65 protein levels [ 84 , 85 ]. Another study reported that paeoniflorin blocked chondrocyte apoptosis induced by IL-1β by downregulating Bcl2 and Bcl-2-associated X-protein levels as well as caspase 3 activity; paeoniflorin also regulated protein kinase B signaling by increasing phosphorylation of this protein [ 86 ].…”